Expression, purification, characterization and crystallization of a recombinant human cytosolic β-glucosidase produced in insect cells

Protein Expression and Purification

Volumn 52, Issue 1, 2007, Pages 96-103

  Fleury, Damien ^a   Domaingue, Priscilla ^a   Gillard, Corinne ^a   Touitou, Robert ^a   Mollat, Patrick ^a  

^a Prostrakan   (France)

Author keywords

First crystals; Heterologous expression system; His tag; Human cytosolic glucosidase

Indexed keywords

BETA GLUCOSIDASE; RECOMBINANT PROTEIN;

ANIMAL; ARMYWORM; ARTICLE; CELL LINE; CHEMISTRY; GEL CHROMATOGRAPHY; GENETIC TRANSFECTION; GENETICS; HUMAN; INSECT; ION EXCHANGE CHROMATOGRAPHY; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; POLYMERASE CHAIN REACTION; SOLUBILITY; X RAY CRYSTALLOGRAPHY;

ANIMALS; BETA-GLUCOSIDASE; CELL LINE; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, ION EXCHANGE; CLONING, MOLECULAR; CRYSTALLOGRAPHY, X-RAY; HUMANS; INSECTS; POLYMERASE CHAIN REACTION; RECOMBINANT PROTEINS; SOLUBILITY; SPODOPTERA; TRANSFECTION;

INSECTA;

EID: 33845965737     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2006.08.014     Document Type: Article

References (18)

1. Henrissat B., and Davies G. Structural and sequence-based classification of glycoside hydrolases. Curr. Opin. Struct. Biol. 5 (1997) 637-644
2. Neufeld E.F. Lysosomal storage diseases. Annu. Rev. Biochem. 60 (1991) 257-280
3. Daniels L.B., Coyle P.J., Chiao Y., and Glew R. Purification and characterization of a cytosolic broad specificity β-glucosidase from human liver. J. Biol. Chem. 256 (1981) 13004-13013
4. Lambert N., Kroon P.A., Faulds C.B., Plumb G.W., McLauchlan W.R., Day A.J., and Williamson G. Purification of cytosolic beta-glucosidase from pig liver and its reactivity towards flavonoid glycosides. Biochim. Biophys. Acta 1435 1-2 (1999) 110-116
5. de Graaf M., van Veen I.C., van der Meulen-Muileman I.H., Gerritsen W.R., Pinedo H.M., and Haisma H.J. Cloning and characterization of human liver cytosolic beta-glycosidase. Biochem. J. 356 (2001) 907-910
6. Glew R.H., Gopalan V., Forsyth G., and Vanderjagt D.J. The mammalian cytosolic broad specificity β-glucosidase. In: Comstock J.M. (Ed). β-Glucosidases: Biochemistry and Molecular Biology (1993), American Chemical Society, Washington, DC 83-112
7. Gopalan V., Pastuszyn A., Galey Jr. W.R., and Glew R.H. Exolitic hydrolysis of toxic plant glucosides by guinea pig cytosolic β-glucosidase. J. Biol. Chem. 267 (1992) 14027-14032
8. LaMarco K., and Glew R.H. Hydrolysis of naturally occurring β-glucoside by a broad specificity beta-glucosidase from liver. Biochem. J. 237 (1986) 469-476
9. Berrin J.G., McLauchlan W.R., Needs P., Williamson G., Puigserver A., Kroon P.A., and Juge N. Functional expression of human liver cytosolic beta-glucosidase in Pichia pastoris: insights into its role in the metabolism of dietary glucosides. Eur. J. Biochem. 269 1 (2002) 249-258
10. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriohage T4. Nature 227 (1970) 680-685
11. Aguilar C.F., Sanderson I., Moracci M., Ciaramella M., Nucci R., Rossi M., and Pearl L.H. Crystal structure of the beta-glycosidase from the hyperthermophilic archeon Sulfolobus solfataricus: resilience as a key factor in thermostability. J. Mol. Biol. 271 (1997) 789-802
12. Barrett T., Suresh C.G., Tolley S.P., Dodson E.J., and Hughes M.A. The crystal structure of a cyanogenic beta-glucosidase from white clover, a family 1glycosyl-hydrolase. Structure 3 (1995) 951-960
13. Burmeister W.P., Cottaz S., Driguez H., Iori R., Palmieri S., and Henrissat B. The crystal structures of Sinapis alba myrosinase and a covalent glycosyl-enzyme intermediate provide insights into the substrate recognition and active-site machinery of an S-glycosidases. Structure 5 (1997) 663-675
14. Chi Y.-I., Martinez-Cruz L.A., Jancarik L., Swanson R.V., Robertson D.E., and Kim S.H. Crystal structure of the beta-glycosidase from the hyperthermophile Thermosphaera aggregans: insights into its activity and thermostability. FEBS Lett. 445 (1999) 375-383
15. Sanz-Aparicio J., Hermoso J.A., Martinez-Ripoll M., Lequerica J.L., and Polaina J. Crystal structure of beta-glucosidase A from Bacillus polymyxa: insight into the catalytic activity in family 1 glycosyl hydrolases. J. Mol. Biol. 275 (1998) 491-502
16. Wiesmann C., Beste G., Hengstenberg W., and Schulz G.E. The 3-dimensional structure of 6-phospho-beta-galactosidase from Lactococcus lactis. Structure 3 (1995) 961-968
17. Czjzek M., Cicek M., Zamboni V., Burmeister W.P., Bevan D.R., and Henrissat B. A. Esen Crystal structure of a monocotyledon (maize ZMGlu1) beta-glucosidase and a model of its complex with p-nitrophenyl beta-d-thioglucoside. Biochem. J. 354 (2001) 37-46
18. Berrin J.G., Czjek M., Kroon P.A., MCLauchlan W.R., Puigserver A., Williamson G., and Juge N. Substrate (aglycone) specificity of human cytosolic β-glucosidase. Biochem. J. 373 (2003) 41-48