Respirable quartz and kaolin alumino-silicate induction of in vitro cytotoxicity and apoptosis in the presence of surfactant or serum: Caveats to bioassay interpretation

Annals of Occupational Hygiene

Volumn 46, Issue , 2002, Pages 50-52

  Gao, N ^a   Keane, M J ^a   Ong, T ^a   Ye, J ^a   Martin, J ^a   Miller, W E ^a   Wallace, W E ^a  

^a NATIONAL INSTITUTE FOR OCCUPATIONAL SAFETY AND HEALTH   (United States)

Author keywords

apoptosis; DPPC surfactant; fetal bovine serum; kaolin; lactate dehydrogenase; NR 8383 cells; quartz; TUNEL

Indexed keywords

ASSAYS; BIOASSAY; BIOLOGICAL ORGANS; BODY FLUIDS; CELL CULTURE; CELL DEATH; CYTOTOXICITY; DUST; KAOLIN; MACROPHAGES; MAMMALS; MOBILE SECURITY; PHOSPHOLIPIDS; PHYSIOLOGY; RATS; SILICATES; SURFACE ACTIVE AGENTS;

ALVEOLAR MACROPHAGES; CELL CULTURE MEDIUMS; DIPALMITOYL PHOSPHATIDYLCHOLINE; FETAL BOVINE SERUM; LACTATE DEHYDROGENASE; PROPHYLACTIC COMPONENTS; PULMONARY SURFACTANTS; TUNEL;

QUARTZ;

EID: 33845943337     PISSN: 00034878     EISSN: 14753162     Source Type: Journal    
DOI: 10.1093/annhyg/46.suppl_1.50     Document Type: Conference Paper

References (11)

1. Barrett EG, Johnston C, Oberdorster G, Finkelstein JN. (1999) Silica binds serum proteins resulting in a shift of the dose-response for silica-induced chemokine expression in an alveolar type II cell line. Toxicol Appl Pharmacol; 161: 111-22.
2. Gao N, Keane MJ, Ong T, Wallace WE. (2000) Effects of simulated pulmonary surfactant on the cytotoxicity and DNA-damaging activity of respirable quartz and kaolin. J Toxicol Environ Health; 60A: 153-67.
3. Gao N, Keane MJ, Ong T, Ye J, Miller WE, Wallace WE. (2001) Effects of phospholipid surfactant on apoptosis induction by respirable quartz and kaolin in NR8383 rat pulmonary macrophages. Toxicol Appl Pharmacol; 175: 217-25.
4. Hill CA, Wallace WE, Keane MJ, Mike PS. (1995) The enzymatic removal of a surfactant coating from quartz and kaolin by P388D1 cells. Cell Biol Toxicol; 11: 119-28.
5. IARC. (1997) IARC monographs on the evaluation of carcinogenic risks to humans: no. 68: silica, some silicates, coal dust, and para-aramid fibrils. Lyon, France: IARC. pp. 41-242.
6. Leigh J, Wang H, Bonin A, Peters M, Rean X. (1997) Silicainduced apoptosis in alveolar and granulomatose cells in vivo. Environ Health Perspect; 105 (suppl. 5): 1241-5.
7. Liu XR, Keane MJ, Harrison JC, Cilento EV, Ong T, Wallace WE. (1998) Phospholipid surfactant adsorption by respirable quartz and in vitro expression of cytotoxicity and DNA damage. Toxicol Lett; 96/97: 77-84.
8. Schulz CO. (1993) Silicon and silicates including asbestos. In Clayton GD, Clayton FE, editors. Patty's industrial hygiene and toxicology 1993, Vol. II, Part A. New York, NY: John Wiley & Sons.
9. Vallyathan V, Schwegler D, Reasor M, Stettler L, Clere J, Green FHY. (1988) Comparative in vitro cytotoxicity and relative pathogenicity of mineral dusts. Ann Occup Hyg; 32: 279-89.
10. Wallace WE, Vallyathan V, Keane MJ, Robinson V. (1985) In vitro biologic toxicity of native and surface-modified silica and kaolin. J Toxicol Environ Health; 16: 415-24.
11. Wallace WE, Keane MJ, Hill CA, Vallyathan V, Regad ED. (1992) Contrasting respirable quartz and kaolin retention of lecithin surfactant and expression of membranolytic activity following phospholipase A2 digestion. J Toxicol Environ Health; 37: 391-409.