Development of an improved species specific PCR test for detection of Haemophilus parasuis

Veterinary Microbiology

Volumn 119, Issue 2-4, 2007, Pages 266-276

  Angen, Øystein ^a   Oliveira, Simone ^b   Ahrens, Peter ^a   Svensmark, Birgitta ^c   Leser, Thomas D ^a  

^a TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

^b UNIVERSITY OF MINNESOTA   (United States)

^c Danish Meat Association   (Denmark)

Author keywords

Haemophilus parasuis; Identification; PCR detection

Indexed keywords

DNA 16S; PRIMER DNA;

ACTINOBACILLUS; ACTINOBACILLUS INDOLICUS; ACTINOBACILLUS MINOR; ACTINOBACILLUS PORCINUS; AMPLICON; ARTICLE; BACTERIAL GENETICS; BACTERIAL STRAIN; BACTERIUM CULTURE; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; DNA SEQUENCE; ESCHERICHIA COLI; GENETIC ANALYSIS; GEOGRAPHIC DISTRIBUTION; HAEMOPHILUS PARASUIS; INFLAMMATION; INTERMETHOD COMPARISON; NONHUMAN; PASTEURELLACEAE; POLYMERASE CHAIN REACTION; RELIABILITY; SENSITIVITY ANALYSIS; SENSITIVITY AND SPECIFICITY; SEPTICEMIA; SEROTYPE; SPECIES DIFFERENCE; STRAIN DIFFERENCE; SWINE; UPPER RESPIRATORY TRACT;

ANIMALS; COLONY COUNT, MICROBIAL; DNA PRIMERS; DNA, BACTERIAL; DNA, RIBOSOMAL; HAEMOPHILUS INFECTIONS; HAEMOPHILUS PARASUIS; NOSE; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; RNA, RIBOSOMAL, 16S; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS, DNA; SEROTYPING; SPECIES SPECIFICITY; SWINE; SWINE DISEASES;

ESCHERICHIA COLI; HAEMOPHILUS PARASUIS; PASTEURELLACEAE;

EID: 33845903837     PISSN: 03781135     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.vetmic.2006.10.008     Document Type: Article

References (20)

1. Aarestrup F.M., Seyfarth A.M., and Angen Ø. Antimicrobial susceptibility of Haemophilus parasuis and Histophilus somni from pigs and cattle in Denmark. Vet. Microbiol. 101 (2004) 143-146
2. Angen Ø., Ahrens P., and Bisgaard M. Phenotypic and genotypic characterization of Mannheimia (Pasteurella) haemolytica-like strains isolated from diseased animals in Denmark. Vet. Microbiol. 84 (2002) 103-114
3. Angen Ø., Ahrens P., and Tegtmeier C. Development of a PCR test for identification of Haemophilus somnus in pure and mixed cultures. Vet. Microbiol. 63 (1998) 39-48
4. Angen Ø., Svensmark B., and Mittal K.R. Serological characterization of Danish Haemophilus parasuis isolates. Vet. Microbiol. 103 (2004) 255-258
5. Blackall P., Trott D.J., Rapp-Gabrielson V., and Hampson D.J. Analysis of Haemophilus parasuis by multilocus enzyme electrophoresis. Vet. Microbiol. 56 (1997) 125-134
6. Del Río W.L., Gutiérrez C.B., and Ferri E.F.R. Value of indirect haemagglutination and coagglutination tests for serotyping Haemophilus parasuis. J. Clin. Microbiol. 41 (2003) 880-882
7. Kielstein P., and Rapp-Gabrielson V.J. Designation of 15 serovars of Haemophilus parasuis on the basis of immunodiffusion using heat-stable antigen extracts. J. Clin. Microbiol. 30 (1992) 862-865
8. Kielstein P., and Wuthe H.-H. Actinobacillus pleuropneumoniae, Haemophilus parasuis und verwandte Bakterien aus Organen von Schweinen in Schleswig-Holstein. Tierärtzl. Umsch. 53 (1998) 250-258
9. Kielstein P., Wuthe H.-H., Angen Ø., Mutters R., and Ahrens P. Phyenotypic and genetic characterization of NAD-dependent Pasteurellaceae from the respiratory tract of pigs and their possible pathogenetic importance. Vet. Microbiol. 81 (2001) 243-255
10. L'Abee-Lund T.M., Heiene R., Friis N.F., Ahrens P., and Sørum H. Mycoplasma canis and urogenital disease in dogs in Norway. Vet. Rec. 153 (2003) 231-235
11. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S., Jobb G., Forster W., Brettske I., Gerber S., Ginhart A.W., Gross O., Grumann S., Hermann S., Jost R., Konig A., Liss T., Lussmann R., May M., Nonhoff B., Reichel B., Strehlow R., Stamatakis N., Vilbig A., Lenke M., Ludwig T., Bode A., and Schleifer K.-H. ARB: a software environment for sequence data. Nucl. Acids Res. 32 (2004) 1363-1371
12. Møller K., Andersen L.V., Christensen G., and Kilian M. Optimalization of the detection of NAD dependent Pasteurellaceae from the respiratory tract of slaughterhouse pigs. Vet. Microbiol. 36 (1993) 261-271
13. Møller K., Fussing V., Grimont P.A.D., Paster B.J., Dewhirst F.E., and Kilian M. Actinobacillus minor sp. nov., Actinobacillus porcinus sp. nov., and Actinobacillus indolicus sp. nov., three new V factor-dependent species from the respiratory tract of pigs. Int. J. Syst. Bacteriol. 46 (1996) 951-956
14. Møller K., and Kilian M. V factor-dependent members of the family Pasteurellaceae in the porcine upper respiratory tract. J. Clin. Microbiol. 28 (1990) 2711-2716
15. Nielsen R. Serological and immunological studies of pleuropneumonia of swine caused by Haemophilus parasuis. Acta Vet. Scand. 15 (1974) 80-89
16. Oliveira S., Galina L., and Pijoan C. Development of a PCR test to diagnose Haemophilus parasuis infections. J. Vet. Diagn. Invest. 13 (2001) 495-501
17. Oliveira S., Blackall P., and Pijoan C. Characterization of the diversity of Haemophilus parasuis field isolates by use of serotyping and genotyping. Am. J. Vet. Res. 64 (2003) 435-442
18. Oliveira S., and Pijoan C. Haemophilus parasuis: new trends on diagnosis, epidemiology and control. Vet. Microbiol. 99 (2004) 1-12
19. Rapp-Gabrielson V.J., and Gabrielson D.A. Prevalence of Haemophilus parasuis serovars among isolates from swine. Am. J. Vet. Res. 53 (1992) 659-664
20. Vigre H., Angen Ø., Barfod K., Lavritsen D.T., and Sørensen V. Transmission of Actinobacillus pleuropneumoniae in pigs under field-like conditions: emphasis on tonsillar colonisation and passively acquired colostral antibodies. Vet. Microbiol. 89 (2002) 151-159