Brief coincubation of gametes in porcine in vitro fertilization: Role of sperm:oocyte ratio and post-coincubation medium

Theriogenology

Volumn 67, Issue 3, 2007, Pages 620-626

  Gil, M A ^a   Alminana C ^a   Cuello, C ^a   Parrilla, I ^a   Roca, J ^a   Vazquez, J M ^a   Martinez, E A ^a  

^a UNIVERSITY OF MURCIA   (Spain)

Author keywords

Coincubation time; In vitro fertilization; Oocyte; Porcine

Indexed keywords

ANIMAL; ANIMAL DISEASE; ARTICLE; CULTURE MEDIUM; FEMALE; FERTILITY; FERTILIZATION IN VITRO; MALE; METABOLISM; METHODOLOGY; OOCYTE; PHYSIOLOGY; SPERM PRESERVATION; SPERMATOZOON; SPERMATOZOON COUNT; SWINE; TIME;

ANIMALS; CULTURE MEDIA; FEMALE; FERTILIZATION IN VITRO; MALE; OOCYTES; SEMEN PRESERVATION; SPERM COUNT; SPERM-OVUM INTERACTIONS; SPERMATOZOA; SWINE; TIME FACTORS;

SUIDAE;

EID: 33845903034     PISSN: 0093691X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.theriogenology.2006.09.022     Document Type: Article

References (28)

1. Niwa K. Effectiveness of in vitro maturation and in vitro fertilization techniques in pigs. J Reprod Fertil 48 (1993) 49-59
2. Funahashi H., and Day B.N. Advances in in vitro production of pig embryos. J Reprod Fertil 52 (1997) 271-283
3. Prathers R.S., and Day B.N. Practical considerations for the in vitro production of pig embryos. Theriogenology 49 (1998) 23-32
4. Day B.N. Reproductive biotechnologies: current status in porcine reproduction. Anim Reprod Sci 60-61 (2000) 161-172
5. Funahashi H. Polyspermic penetration in porcine IVM-IVF systems. Reprod Fertil Dev 15 (2003) 167-177
6. Abeydeera L.R. In vitro production of embryos in swine. Theriogenology 57 (2002) 256-273
7. Fraser L.R. Ionic control of sperm function. Reprod Fertil Dev 7 (1995) 905-925
8. Abeydeera L.R., and Day B.N. In vitro penetration of pig oocytes in a modified Tris-buffered medium: effect of BSA, caffeine and calcium. Theriogenology 48 (1997) 537-544
9. Martinez-Madrid B., Domínguez E., Alonso C., Diaz C., Garcia P., and Sanchez R. Effect of IVF medium and sperm concentration on fertilization parameters. Proceedings of the sixth international conference on pig reproduction. University of Missouri, Columbia, MO (2001) 75
10. Kidson A., Colebrander B., Verheijden J.H.M., and Bevers M.M. Polyspermy in the pig is dependent on both IVF medium and sperm dose during fertilization in vitro. Proceedings of the sixth international conference on pig reproduction. University of Missouri, Columbia, MO (2001) 74
11. Rath D. Experiments to improve in vitro fertilization techniques for in vivo matured porcine oocytes. Theriogenology 37 (1992) 885-886
12. Xu X., Seth P.C., Harbison D.S., Cheung A.P., and Foxcroft G.R. Semen dilution for assessment of boar ejaculate quality in pig IVM and IVF systems. Theriogenology 46 (1996) 1325-1337
13. Gil M.A., Ruiz M., Cuello C., Vazquez J.M., Roca J., and Martinez E.A. Influence of sperm:oocyte ratio during in vitro fertilization of in vitro matured cumulus-intact pig oocytes on fertilization parameters and embryo development. Theriogenology 61 (2004) 551-560
14. Wang W.H., Abeydeera L.R., Okuda K., and Niwa K. Penetration of porcine oocytes during maturation in vitro by cryopreserved ejaculated spermatozoa. Biol Reprod 50 (1994) 510-515
15. Abeydeera L.R., and Day B.N. Fertilization and subsequent development in vitro of pig oocytes inseminated in a modified tris-buffered medium with frozen-thawed ejaculated spermatozoa. Biol Reprod 57 (1997) 729-734
16. Gil M.A., Roca J., Cremades T., Hernandez M., Vazquez J.M., Rodriguez-Martinez H., et al. Does multivariate analysis of post-thaw sperm characteristics accurately estimate in vitro fertility of boar individual ejaculates?. Theriogenology 64 (2005) 305-316
17. Coy P., Martinez E., Ruiz S., Vazquez J.M., Roca J., and Matas C. In vitro fertilization of pig oocytes alter different coincubation intervals. Theriogenology 39 (1993) 1201-1208
18. Ocampo M.B., Ocampo L.C., Mori T., Ueda J., and Kanagawa H. Timing of sequential changes in chromosome configurations during the second meiotic division and cytoplasmic events of pig oocytes matured and fertilized in vitro. Anim Reprod Sci 34 (1994) 281-288
19. Marchal R., Pelaez J., Terque M., and Mermillod P. Effect of sperm survival and CTC staining pattern on in vitro fertilization of porcine oocytes. Theriogenology 57 (2002) 1917-1927
20. Gil M.A., Ruiz M., Vazquez J.M., Roca J., Day B.N., and Martinez E.A. Effect of short periods of sperm-oocyte coincubation during in vitro fertilization on embryo development in pigs. Theriogenology 62 (2004) 544-552
21. Grupen C.G., and Nottle M.B. A simple modification of the in vitro fertilization procedure. Theriogenology 53 (2000) 422
22. Almiñana C., Gil M.A., Cuello C., Roca J., Vazquez J.M., Rodriguez-Martinez H., et al. Adjustments in IVF system for individual boars: value of additives and time of sperm-oocyte co-incubation. Theriogenology 64 (2005) 1783-1796
23. Funahashi H., and Romar R. Reduction of the incidence of polyspermic penetration into porcine oocytes by pre-treatment of fresh spermatozoa with adenosine and a transient co-incubation of the gametes with caffeine. Reproduction 128 (2004) 789-800
24. Hunter R.H.F., and Dziuk P.J. Sperm penetration of pig eggs in relation to the timing of ovulation and insemination. J Reprod Fertil 15 (1968) 199-208
25. Petters R.M., and Wells K.D. Culture of pig embryos. J Reprod Fertil 48 (1993) 61-73
26. Roca J., Carvajal G., Lucas X., Vazquez J.M., and Martinez E.A. Fertility of weaned sows after deep intrauterine insemination with a reduced number of frozen-thawed spermatozoa. Theriogenology 60 (2003) 77-87
27. Nagy S., Jansen J., Topper E.K., and Gadella B.M. A triple-stain flow cytometric method to assess plasma and acrosome membrane integrity of cryopreserved bovine sperm immediately after thawing in presence of egg-yolk particles. Biol Reprod 68 (2003) 1828-1835
28. Funahashi H., Fujiwara T., and Nagai T. Modulation of the function of boar spermatozoa via adenosine and fertilization promoting peptide receptors reduces the incidence of polyspermic penetration into porcine oocytes. Biol Reprod 63 (2000) 1157-1163