Molecular detection of Plasmodiophora brassicae, causal agent of clubroot of crucifers, in plant and soil

Plant Disease

Volumn 91, Issue 1, 2007, Pages 80-87

  Cao, Tiesen ^a   Tewari, Jalpa ^a   Strelkov, Stephen E ^a  

^a UNIVERSITY OF ALBERTA   (Canada)

Author keywords

Oilseed rape

Indexed keywords

DISEASES; DNA; MICROORGANISMS; MOLECULAR BIOLOGY;

OILSEED RAPE; P. BRASSICAE; RIBOSOMAL RNA (RRNA);

PLANTS (BOTANY);

DISEASES; MICROORGANISMS; NUCLEIC ACIDS; PLANTS;

BACTERIA (MICROORGANISMS); BRASSICA NAPUS; BRASSICA NAPUS VAR. NAPUS; FUNGI; PLASMODIOPHORA BRASSICAE;

EID: 33845870140     PISSN: 01912917     EISSN: None     Source Type: Journal    
DOI: 10.1094/PD-91-0080     Document Type: Article

References (36)

1. Altschul, S. F., Gish, W., Miller, W., Myers, E. W., and Lipman, D. J. 1990. Basic local alignment search tool. J. Mol. Biol. 215:403-410.
2. Bansal, V. K., Blenis, P., Stringam, G. R., Thiagarajah, M. R., and Tewari, J. P. 2002. Screening of Brassica napus against blackleg caused by Leptosphaeria maculans: effects of inoculum concentration, subculturing of the pathogen, and time of disease screening. Can. J. Plant Pathol. 24:323-326.
3. Buczacki, S. T. 1983. Plasmodiophora. An inter-relationship between biological and practical problems. Pages 161-191 in: Zoosporic Plant Pathogens. S. T. Buczacki, ed. Academic Press, London.
4. Castlebury, L. A., Maddox, J. V., and Glawe, D. A. 1994. A technique for the extraction and purification of viable Plasmodiophora brassicae resting spores from host root tissue. Mycologia 86:458-460.
5. Clayton, J. S., Erlich, W. A., Cann, D. B., Day, J. H., and Marshall, I. B. 1977. Soils of Canada, Vol. II. Agriculture Canada. Ottawa, ON, Canada.
6. Colhoun, J, 1957. A technique for examining soil for the presence of Plasmodiophora brassicae Woron. Ann. Appl. Biol. 45:559-565.
7. Donald, E. C., Porter, I. J., and Lancaster, R. A. 2001. Band incorporation of fluazinam (shirlan) into soil to control clubroot of vegetable Brassica crops. Aust. J. Exp. Agric. 41:1223-1226.
8. Faggian, R., Bulman, S. R., Lawrie, A. C., and Porter, I. J. 1999. Specific polymerase reaction primers for the detection of Plasmodiophora brassicae in soil and water. Phytopathology 89:392-397.
9. Hildebrand, P. D., and McRae K. B. 1998. Control of clubroot caused by Plasmodiophora brassicae with nonionic surfactants. Can. J. Plant Pathol. 20:1-11.
10. Ito, S., Maehara, T., Maruno, E., Tanaka, S., Kameya-Iwaki, M., and Kishi, F. 1999. Development of a PCR-based assay for the detection of Plasmodiophora brassicae in soil. J. Phytopathol. 147:83-88.
11. Ito, S., Maehara, T., Tanaka, S., Kameya-Iwaki, M., Yano, S., and Kishi, F. 1997. Cloning of a single-copy DNA sequence unique to Plasmodiophora brassicae. Physiol. Mol. Plant Pathol. 50:289-300.
12. Jones, D. R., and Ingram, D. S. 1982. Characterization of isolates derived from single resting spores of Plasmodiophora brassicae and studies of their interaction. Plant Pathol. 31:239-246.
13. Karling, J. S. 1968. The Plasmodiophorales. Hafner Publishing Company, Inc., New York.
14. Lange, L., Heide, M., Hobolth, L., and Olson, L. W. 1989. Serological detection of Plasmodiophora brassicae by dot immunobinding and visualization of the serological reaction by scanning electron microscopy. Phytopathology 79:1066-1071.
15. MacFarlane, I. 1952. Factors affecting the survival of Plasmodiophora brassicae Wor. in the soil and its assessment by a host test. Ann. Appl. Biol. 39:239-256.
16. Melville, I. E., and Hawken, R. W. 1967. Soil testing for club root in Devon and Cornwall. Plant Pathol. 16:145-147.
17. Murakami, H., Tsushima, S., Akimoto, T., Murakami, K., Goto, I., and Shishido, Y. 2000. Effects of growing leafy daikon (Raphanus sativus) on populations of Plasmodiophora brassicae (clubroot). Plant Pathol. 49:584-589.
18. Murakami, H., Tsushima, S., Kuroyanagi, Y., and Shishido, Y. 2002. Reduction of resting spore density of Plasmodiophora brassicae and clubroot disease severity by liming. Soil Sci. Plant Nutr. 48:685-691.
19. Narisawa, K., Shimura, M., Usuki, F, Fukuhara, S., and Hashiba, T. 2005. Effects of pathogen density, soil moisture, and soil pH on biological control of clubroot in Chinese cabbage by Heteroconium chaetospira. Plant Dis. 89:285-290.
20. Rogers, S. O., and Bendich, A. J. 1985. Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues. Plant Mol. Biol. 5:69-76.
21. Rogers, S. O., and Bendich, A. J. 1994. Extraction of total cellular DNA from plants, algae and fungi. Plant Mol. Biol. Manual D1:1-8.
22. Rozen, S., and Skaletsky, H. J. 2000. Primer3 on the WWW for general users and for biologist programmers. Pages 365-386 in: Bioinformatics Methods and Protocols: Methods in Molecular Biology. S. Krawetz and S. Misener, eds. Humana Press, Totowa, NJ.
23. Russel, P. J., Wagner, S., Rodland, K. D., Feinbaum, R. L., Russel, J. P., Bret-Harte, M. S., Free, S. J., and Metzenberg, R. L. 1984. Organization of the ribosomal ribonucleic acid genes in various wild-type strains and wild-collected strains of Neurospora. Mol. Gen. Genet. 196:275-282.
24. Shimotori, H., Yanagida, H., Enomoto, Y., Igarashi, K., Yoshinari, M., and Umemoto, M., 1996. Evaluation of benzenesulfonanilide derivatives for the control of crucifers clubroot. J. Pestic. Sci. 21:31-35.
25. Strelkov, S. E., Cao, T., Manolii, V. P., Lange, R. M., Smith-Degenhardt, E., Orchard, D., and Tewari, J. P. 2006. Incidence of clubroot on canola in Alberta in 2005. Can. Plant Dis. Surv. 86:91-93.
26. Strelkov, S. E., Tewari, J. P., Hartman, M., and Orchard, D. 2005. Clubroot on canola in Alberta in 2003 and 2004. Can. Plant Dis. Surv. 85:72-73.
27. Strelkov, S. E., Tewari, J. P., and Smith-Degenhardt, E. Characterization of Plasmodiophora brassicae populations from Alberta, Canada. Can. J. Plant Pathol. In press.
28. Takahashi, K., and Yamaguchi, T. 1988. A method for assessing the pathogenic activity of resting spores of Plasmodiophora brassicae by fluorescence microscopy. Ann. Phytopathol. Soc. Jpn. 54:466-475.
29. Takahashi, K., and Yamaguchi, T. 1989. Assessment of pathogenicity of resting spores of Plasmodiophora brassicae in soil by fluorescence microscopy. Ann. Phytopathol. Soc. Jpn. 55:621-628.
30. Tewari, J. P., Strelkov, S. E., Orchard, D., Hartman, M., Lange, R. M., and Turkington, T. K. 2005. Identification of clubroot of crucifers on canola (Brassica napus) in Alberta. Can. J. Plant Pathol. 27:143-144.
31. Voonips, R. E. 1995. Plasmodiophora brassicae: aspects of pathogenesis and resistance in Brassica oleracea. Euphytica 83:139-146.
32. Wakeham, A. J., and White, J. G. 1996. Serological detection in soil of Plasmodiophora brassicae resting spores. Physiol. Mol. Plant Pathol. 48:289-303.
33. Wallenhammar, A. C., and Arwidsson, O. 2001. Detection of Plasmodiophora brassicae by PCR in naturally infested soils. Eur. J. Plant Pathol. 107:313-321.
34. Ward, E., and Adams, M. J. 1998. Analysis of ribosomal DNA sequences of Polymyxa species and related fungi and the development of genus- and species-specific PCR primers. Mycol. Res. 102:965-974.
35. Williams, P. H. 1966. A system for the determination of races of Plasmodiophora brassicae that infect cabbage and Rutabaga. Phytopathology 56:624-626.
36. Yeates, C., Gillings, M. R., Davison, A. D., Altavilla, N., and Veal, D. A. 1998. Methods for microbial DNA extraction from soil for PCR amplification. Biol. Procedures Online 1:40-47.