Isoelectric focusing is superior to immunofixation electrophoresis in diagnosing CNS inflammation

Acta Neurologica Scandinavica

Volumn 115, Issue 2, 2007, Pages 122-125

  Mygland, A ^a,b,c   Trydal, T ^a   Vinje, B U ^a   Vedeler, C ^c,d  

^a Sørlandet Sykehus ^*  (Norway)

^b OSLO UNIVERSITY HOSPITAL   (Norway)

^c UNIVERSITY OF BERGEN   (Norway)

^d HAUKELAND UNIVERSITY HOSPITAL   (Norway)

Author keywords

Cerebrospinal fluid; Electrophoresis; Immunofixation; Isoelectric focusing; Multiple sclerosis

Indexed keywords

OLIGOCLONAL BAND;

ADULT; AGED; AUTOIMMUNE DISEASE; BLOOD SAMPLING; CENTRAL NERVOUS SYSTEM DISEASE; CENTRAL NERVOUS SYSTEM INFECTION; CEREBROSPINAL FLUID; CONTROLLED STUDY; ELECTROPHORESIS; HIGH RESOLUTION AGAROSE ELECTROPHORESIS; HUMAN; IMMUNOFIXATION PEROXIDASE ELECTROPHORESIS; INFLAMMATION; INTERMETHOD COMPARISON; ISOELECTRIC FOCUSING; MAJOR CLINICAL STUDY; MEDICAL RECORD; MULTIPLE SCLEROSIS; NEUROLOGY; REVIEW; SAMPLING; SENSITIVITY AND SPECIFICITY; STAINING;

ADOLESCENT; ADULT; AGED; AGED, 80 AND OVER; CENTRAL NERVOUS SYSTEM DISEASES; COHORT STUDIES; ELECTROPHORESIS; ENCEPHALITIS; FOLLOW-UP STUDIES; HUMANS; MIDDLE AGED; MYELITIS; OLIGOCLONAL BANDS; PREDICTIVE VALUE OF TESTS; RETROSPECTIVE STUDIES;

EID: 33845868772     PISSN: 00016314     EISSN: 16000404     Source Type: Journal    
DOI: 10.1111/j.1600-0404.2006.00763.x     Document Type: Review

References (17)

1. MCDONALD WI, COMPSTON A, EDAN G et al. Recommended diagnostic criteria for multiple sclerosis: guidelines from the International Panel on the diagnosis of multiple sclerosis. Ann Neurol 2001;50:121-7.
2. FREEDMAN MS, THOMPSON EJ, DEISENHAMMER F et al. Recommended standard of cerebrospinal fluid analysis in the diagnosis of multiple sclerosis. A consensus statement. Arch Neurol 2005;62:865-70.
3. CAVUOTI D, BASKIN L, JIALAL I. Detection of oligoclonal bands in cerebrospinal fluid by immunofixation electrophoresis. Am J Clin Pathol 1998;109:585-8.
4. RICHARD S, MIOSSEC V, MOREAU JF, TAUPIN JL. Detection of oligoclonal immunoglobulins in cerebrospinal fluid by an immunofixation-peroxidase method. Clin Chem 2002;48:167-73.
5. KEREN DF. Optimizing detection of oligoclonal bands in cerebrospinal fluid by use of isoelectric focusing with IgG immunoblotting. Am J Clin Pathol 2003;120:649-51.
6. FROMONT A, COUVREUR G, GUIGUET M, GIROUD M, CAUDIE C, MOREAU T. Immunofixation compared to isoelectric focusing in the detection of oligoclonal bands in cerebrospinla fluid of multiple sclerosis patients. Rev Neurol (Paris) 2005;161:1183- 90.
7. KEIR G, LUXTON RW, THOMPSON EJ. Isoelectric focusing of cerebrospinal fluid immunoglobulin G: an annotated update. Ann Clin Biochem 1990;27:436-43.
8. BOURAHOUI A, DE SEZE J, GUTTIEREZ R et al. CSF isoelectrofocusing in a large cohort of MS and other neurological diseases. Eur J Neurol 2004;11:525-9.
9. LUNDING J, MIDGARD R, VEDELER CA. Oligoclonal bands in cerebrospinal fluid: a comparative study of isoelectric focusing, agarose gel electrophoresis and IgG index. Acta Neurol Scand 2000;102:322-5.
10. THOMPSON AJ, HUTCHINSON M, MARTIN EA, MANSFIELD M, WHELAN A, FEIGHERY C. Suspected and clinically definite multiple sclerosis: the relationship between CSF immunoglobulins and clinical course. J Neurol Neurosurg Psychiatry 1985;48:989-94.
11. FORTINI AS, SANDERS EL, WEINSHENKER BG, KATZMANN JA. Cerebrospinal fluid oligoclonal bands in the diagnosis of multiple sclerosis. Isoelectric focusing with IgG immunoblotting compared with high-resolution agarose gel electrophoresis and cerebrospinal fluid IgG index. Am J Clin Pathol 2003;120:672-5.
12. COLE SR, BECK RW, MOKE PS, KAUFMAN DI, TOURTELLOTTE WW. The predictive value of CSF oligoclonal banding for MS 5 years after optic neuritis. Optic Neuritis Study Group. Neurology 1998;51:885-7.
13. MOULIN D, PATY DW, EBERS GC. The predictive value of cerebrospinal fluid electrophoresis in 'possible' multiple sclerosis. Brain 1983;106:809-16.
14. MCLEAN BN, LUXTON RW, THOMPSON EJ. A study of immunoglobulin G in the cerebrospinal fluid of 1007 patients with suspected neurological disease using isoelectric focusing and the Log IgG-Index. A comparison and diagnostic applications. Brain 1990;113:1269-89.
15. DAVIES G, KEIR G, THOMPSON EJ, GIOVANNONI G. The clinical significance of an intrathecal monoclonal immunoglobulin band: a follow-up study. Neurology 2003;60:1163-6.
16. NIEDERWIESER G, BONELLI RM, BRUNNER D et al. Diagnostic accuracy of an agarose gel electrophoretic method in multiple sclerosis. Clin Chem 2001;47:144.
17. VERBEEK MM, DE REUS HP, WEYKAMP CW. Comparison of methods for the detection of oligoclonal IgG bands in cerebrospinal fluid and serum: results of the Dutch Quality Control survey. Clin Chem 2002;48:1578-80.