Analysis of γ-rays induced chromosome aberrations: A fingerprint evaluation with a combination of pan-centromeric and pan-telomeric probes

International Journal of Radiation Biology

Volumn 82, Issue 12, 2006, Pages 869-875

  Benkhaled, L ^a   Barrios, L ^a   Mestres, M ^a   Caballin, M R ^a   Ribas, M ^b   Barquinero, J F ^a  

^a UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

^b HOSPITAL DE LA SANTA CREU I SANT PAU   (Spain)

Author keywords

Acentric fragments; Incomplete chromosome aberrations; Pan telomeric and pan centromeric DNA probes; Radiation quality fingerprints

Indexed keywords

PEPTIDE NUCLEIC ACID;

ADULT; ALPHA RADIATION; ARTICLE; BLOOD SAMPLING; CENTROMERE; CHROMOSOME ABERRATION; CHROMOSOME ANALYSIS; CONTROLLED STUDY; DNA PROBE; FLUORESCENCE IN SITU HYBRIDIZATION; GAMMA RADIATION; HUMAN; HUMAN CELL; LINEAR ENERGY TRANSFER; MALE; NORMAL HUMAN; PRIORITY JOURNAL; RADIATION DOSE; RADIATION EXPOSURE; TELOMERE;

ADULT; CENTROMERE; CHROMOSOME ABERRATIONS; CHROMOSOMES; DNA FINGERPRINTING; DNA PROBES; DOSE-RESPONSE RELATIONSHIP, RADIATION; GAMMA RAYS; HUMANS; IN SITU HYBRIDIZATION, FLUORESCENCE; MALE; MONOCYTES; RADIATION DOSAGE; TELOMERE;

EID: 33845787403     PISSN: 09553002     EISSN: 13623095     Source Type: Journal    
DOI: 10.1080/09553000600979092     Document Type: Article

References (41)

1. Anderson RM, Marsden SJ, Paice SJ, Bristow AE, Kadhim MA, Griffin CS, Goodhead DT. 2003. Transmissible and nontransmissible complex chromosome aberrations characterized by three-color and mFISH define a biomarker of exposure to high-LET alpha particles. Radiation Research 159:40-48.
2. Bauchinger M, Schmid E. 1997. Is there reliable experimental evidence for a chromosomal "fingerprint" of exposure to densely ionizing radiation? Radiation Research 147:506-510.
3. Bauchinger M, Schmid E. 1998. LET dependence of yield ratios of radiation-induced intra- and interchromosomal aberrations in human lymphocytes. International Journal of Radiation Biology 74:17-25.
4. Boei JJ, Vermeulen S, Fomina J, Natarajan AT. 1998. Detection of incomplete exchanges and interstitial fragments in X-irradiated human lymphocytes using a telomeric PNA probe. International Journal of Radiation Biology 73:599-603.
5. Boei JJ, Vermeulen S, Natarajan AT. 2000. Analysis of radiation-induced chromosomal aberrations using telomeric and centromeric PNA probes. International Journal of Radiation Biology 76:163-167.
6. Boei JJ, Vermeulen S, Mullenders LH, Natarajan AT. 2001. Impact of radiation quality on the spectrum of induced chromosome exchange aberrations. International Journal of Radiation Biology 77:847-857.
7. Brenner DJ, Sachs RK. 1994. Chromosomal "fingerprints" of prior exposure to densely ionizing radiation. Radiation Research 140:134-142.
8. Brenner DJ, Okladnikova N, Hande P, Burak L, Geard CR, Azizova T. 2001. Biomarkers specific to densely-ionising (high LET) radiations. Radiation Protection Dosimetry 97:69-73.
9. Chudoba I, Plesch A, Lorch T, Lemke J, Claussen U, Senger G. 1999. High resolution multicolor-banding: A new technique for refined FISH analysis of human chromosomes. Cytogenetics Cell Genetics 84:156-160.
10. Deng W, Morrison DP, Gale KL, Lucas JN. 2000. A comparative study on potential cytogenetic fingerprints for radiation LET in human lymphocytes. International Journal of Radiation Biology 76:1589-1598.
11. Edwards AA, Lindholm C, Darroudi F, Stephan G, Romm H, Barquinero JF, Barrios L, Caballín MR, Roy L, Whitehouse CA, Tawn EJ, Moquet J, Lloyd DC, Voisin P. 2005. Review of translocations detected by FISH for retrospective biological dosimetry applications. Radiation Protection Dosimetry 113:396-402.
12. Fomina J, Darroudi F, Boei JJ, Natarajan AT. 2000. Discrimination between complete and incomplete chromosome exchanges in X-irradiated human lymphocytes using FISH with pancentromeric and chromosome specific DNA probes in combination with telomeric PNA probe. International Journal of Radiation Biology 76:807-813.
13. Fomina J, Darroudi F, Boei JJ, Natarajan AT. 2001. Accurate detection of true incomplete exchanges in human lymphocytes exposed to neutron radiation using chromosome painting in combination with a telomeric PNA probe. International Journal of Radiation Biology 77:1175-1183.
14. Goodhead DT, Thacker J, Cox R. 1993. Effects of radiations of different qualities on cells: Molecular mechanisms of damage and repair. International Journal of Radiation Biology 63:543-556.
15. Hande MP, Azizova TV, Geard CR, Burak LE, Mitchell CR, Khokhryakov VF, Vasilenko EK, Brenner DJ. 2003. Past exposure to densely ionizing radiation leaves a unique permanent signature in the genome. American Journal of Human Genetics 72:1162-1170.
16. Hande MP, Azizova TV, Burak LE, Khokhryakov VF, Geard CR, Brenner DJ. 2005. Complex chromosome aberrations persist in individuals many years after occupational exposure to densely ionizing radiation: An mFISH study. Genes Chromosomes Cancer 44:1-9.
17. Hlatky LR, Sachs RK, Hahnfeldt P. 1992. The ratio of dicentrics to centric rings produced in human lymphocytes by acute low-LET radiation. Radiation Research 129:304-308.
18. International Atomic Energy Agency (IAEA). 1986. Chromosomal aberrations analysis for dose-assessment, Technical reports series no. 260 (Vienna: IAEA).
19. Jenner TJ, Delara CM, O'Neill P, Stevens DL. 1993. Induction and rejoining of DNA double-strand breaks in V79-4 mammalian cells following gamma- and alpha-irradiation. International Journal of Radiation Biology 64:265-273.
20. Kodama Y, Nakano M, Ohtaki K, Dongchamp R, Awa AA, Nakamura N. 1997. Estimation of minimal size of translocated chromosome segments detectable by fluorescence in situ hybridization. International Journal of Radiation Biology 71:35-39.
21. Kodama Y, Ohtaki K, Awa AA, Nakano M, Itoh M, Nakamura N. 1999. The F value for chromosome aberrations in atomic bomb survivors does not provide evidence for a primary contribution of neutrons to the dose in Hiroshima. Radiation Research 152:558-562.
22. Lansdorp PM, Verwoerd NP, van de Rijke FM, Dragowska V, Little MT, Dirks RW, Raap AK, Tanke HJ. 1996. Heterogeneity in telomere length of human chromosomes. Human Molecular Genetics 5:685-691.
23. Lindholm C, Romm H, Stephan G, Schmid E, Moquet J, Edwards AA. 2002. Intercomparison of translocation and dicentric frequencies between laboratories in a follow-up of the radiological accident in Estonia. International Journal of Radiation Biology 78:883-890.
24. Lucas JN, Awa A, Straume T, Poggensee M, Kodama Y, Nakano M, Ohtaki K, Weier HU, Pinkel D, Gray J, Littlefield G. 1992. Rapid translocation frequency analysis in man decades after exposure to ionizing radiation. International Journal of Radiation Biology 62:53-63.
25. Lucas JN. 1998. Cytogenetic signature for ionizing radiation. International Journal of Radiation Biology 73:15-20.
26. Lucas JN, Deng W, Oram SW, Hill FS, Durante M, George K, Wu H, Owens CL, Yang T. 1999. Theoretical and experimental tests of a chromosomal fingerprint for densely ionizing radiation based on F ratios calculated from stable and unstable chromosome aberrations. Radiation Research 151:85-91.
27. Lloyd DC, Edwards AA, Prosser JS. 1986. Chromosome aberrations induced in human lymphocytes by in vitro acute X and gamma radiation. Radiation Protection Dosimetry 15:83-88.
28. Mestres M, Caballin MR, Schmid E, Stephan G, Sachs R, Barrios L, Barquinero JF. 2004. Analysis of alpha-particle induced chromosome aberrations in human lymphocytes, using pancentromeric and pan-telomeric probes. International Journal of Radiation Biology 80:737-744.
29. Mitchell CR, Azizova TV, Hande MP, Burak LE, Tsakok JM, Khokhryakov VF, Geard CR, Brenner DJ. 2004. Stable intrachromosomal biomarkers of past exposure to densely ionizing radiation in several chromosomes of exposed individuals. Radiation Research 162:257-263.
30. Natarajan AT, Balajee AS, Boei JJ, Darroudi F, Dominguez I, Hande MP, Meijers M, Slijepcevic P, Vermeulen S, Xiao Y. 1996. Mechanisms of induction of chromosomal aberrations and their detection by fluorescence in situ hybridization. Mutation Research 372:247-258.
31. Rao CR, Chakravarti IM. 1956. Some small sample tests of significance for a Poisson distribution. Biometrics 12:264-282.
32. Sasaki MS, Takatsuji T, Ejima Y. 1998. The F value cannot be ruled out as a chromosomal fingerprint of radiation quality. Radiation Research 150:253-258.
33. Sachs RK, Brenner DJ, Chen AM, Hahnfeldt P, Hlatky LR. 1997. Intra-arm and interarm chromosome intrachanges: Tools for probing the geometry and dynamics of chromatin. Radiation Research 148:330-340.
34. Savage JR, Simpson PJ. 1994. FISH "painting" patterns resulting from complex exchanges. Mutation Research 312:51-60.
35. Speicher MR, Gwyn Ballard S, Ward DC. 1996. Karyotyping human chromosomes by combinatorial multi-fluor FISH. Nature Genetics 12:368-375.
36. Stephens JC, Cavanaugh ML, Gradie MI, Mador ML, Kidd KK. 1990. Mapping the human genome: Current status. Science 250:237-244.
37. Wu H, Durante M, Sachs RK, Yang TC. 1997. Centric rings, acentric rings and excess acentric fragments based on a random-walk interphase chromosome model. International Journal of Radiation Biology 71:487-496.
38. Wu H, George K, Yang TC. 1998. Estimate of true incomplete exchanges using fluorescence in situ hybridization with telomere probes. International Journal of Radiation Biology 73:521-527.
39. Wu H, George K, Yang TC. 1999. Estimate of the frequency of true incomplete exchanges in human lymphocytes exposed to 1 GeV/u Fe ions in vitro. International Journal of Radiation Biology 75:593-599.
40. Wu H, George K, Willingham V, Kawata T, Cucinotta FA. 2001. Comparison of F ratios generated from interphase and metaphase chromosome damage induced by high doses of low- and high-LET radiation. Radiation Research 155:57-62.
41. Wu H, Durante M, Furusawa Y, George K, Kawata T, Cucinotta FA. 2003. M-FISH analysis of chromosome aberrations in human fibroblasts exposed to energetic iron ions in vitro. Advances in Space Research 31:1537-1542.