Development of a novel ELISA for human insulin using monoclonal antibodies produced in serum-free cell culture medium

Clinical Biochemistry

Volumn 40, Issue 1-2, 2007, Pages 98-103

  Even, Megha S ^a   Sandusky, Chad B ^a   Barnard, Neal D ^a   Mistry, Jehangir ^b   Sinha, Madhur K ^b  

^a PHYSICIANS COMMITTEE FOR RESPONSIBLE MEDICINE   (United States)

^b Linco Research Inc   (United States)

Author keywords

Animal use alternatives; Diabetes clinical research; Immunoassay; Insulin ELISA; Insulin monoclonal antibodies; Serum free hybridomas

Indexed keywords

BIOSYNTHETIC HUMAN PROINSULIN; BOVINE INSULIN; BOVINE SERUM ALBUMIN; C PEPTIDE; INSULIN; MONOCLONAL ANTIBODY;

ACCURACY; ADULT; ANTIBODY PRODUCTION; ARTICLE; BLOOD SAMPLING; BLOOD STORAGE; CLINICAL ARTICLE; CLINICAL CHEMISTRY; COMPARATIVE STUDY; CONTROLLED STUDY; CORRELATION ANALYSIS; CROSS REACTION; CULTURE MEDIUM; DIET RESTRICTION; DILUTION; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; HORMONE DETERMINATION; HUMAN; HYBRIDOMA; IN VITRO STUDY; INSULIN BLOOD LEVEL; INSULIN SENSITIVITY; LEAN BODY WEIGHT; LINEAR SYSTEM; MALE; OBESITY; PRIORITY JOURNAL; PROCESS DEVELOPMENT; RELIABILITY; STATISTICAL SIGNIFICANCE; STORAGE TEMPERATURE;

ADULT; AGED; ANIMALS; ANTIBODIES, MONOCLONAL; CATTLE; CULTURE MEDIA, SERUM-FREE; ENZYME-LINKED IMMUNOSORBENT ASSAY; FASTING; FEMALE; HUMANS; INSULIN; MALE; MICE; MIDDLE AGED; POSTPRANDIAL PERIOD; REAGENT KITS, DIAGNOSTIC; REGRESSION ANALYSIS; SENSITIVITY AND SPECIFICITY;

EID: 33845759471     PISSN: 00099120     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.clinbiochem.2006.10.004     Document Type: Article

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