Validation of a Rickettsia prowazekii-specific quantitative real-time PCR cassette and DNA extraction protocols using experimentally infected lice

Annals of the New York Academy of Sciences

Volumn 1078, Issue , 2006, Pages 617-619

  Rozmajzl, Patrick J ^a,c   Houhamdi, Linda ^b   Jiang, Ju ^a   Raoult, Didier ^b   Richards, Allen L ^a  

^a NAVAL MEDICAL RESEARCH CENTER   (United States)

^b AIX MARSEILLE UNIVERSITY   (France)

^c NMRC   (United States)

Author keywords

DNA extraction in lice; PCR studies in lice; Rickettsia prowazekii

Indexed keywords

ANIMAL EXPERIMENT; ANIMAL MODEL; CELL CULTURE; CONFERENCE PAPER; CONTROLLED STUDY; DEATH; DNA EXTRACTION; DNA ISOLATION; EXPERIMENTAL RABBIT; GENE CASSETTE; INOCULATION; INTERMETHOD COMPARISON; LOUSE; NONHUMAN; REAL TIME POLYMERASE CHAIN REACTION; RICKETTSIA PROWAZEKII; RICKETTSIOSIS; SENSITIVITY ANALYSIS; SENSITIVITY AND SPECIFICITY;

PHTHIRAPTERA; RICKETTSIA PROWAZEKII;

EID: 33845717344     PISSN: 00778923     EISSN: 17496632     Source Type: Book Series    
DOI: 10.1196/annals.1374.036     Document Type: Conference Paper

References (3)

1. HOUHAMDI, L., P.E. FOURNIER, R. FAND, et al. 2002. An experimental model of human body louse infection with Rickettsia prowazekii. J. Inf. Dis. 186: 1639-1646.
2. JIANG, J., T.C. CHAN, J.J. TEMENAK, et al. 2004. Development of a quantitative real-time polymerase chain reaction assay specific for Orientia tsutsugamushi. Am. J. Trop. Med. Hyg. 70: 351-356.
3. JIANG, J., J.J. TEMENAK & A.L. RICHARDS. 2003. Real-time PCR duplex assay for Rickettsia prowazekii and Borrelia recurrentis. Ann. N. Y. Acad. Sci. 990: 302-310.