Large systematic errors compromise quantitation of intrinsically unstructured proteins

Analytical Biochemistry

Volumn 360, Issue 2, 2007, Pages 321-323

  Szollosi, E ^a   Hazy E ^a   Szasz Cs ^a   Tompa, P ^a  

^a INSTITUTE OF EXPERIMENTAL MEDICINE   (Hungary)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY; CHEMISTRY;

INTRINSICALLY UNSTRUCTURED PROTEINS;

SYSTEMATIC ERRORS;

INTRINSICALLY UNSTRUCTURED PROTEIN; PROTEIN;

AMINO ACID COMPOSITION; ARTICLE; COLORIMETRY; DIALYSIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN STRUCTURE; QUANTITATIVE ANALYSIS; SYSTEMATIC ERROR; ULTRAVIOLET SPECTROSCOPY;

EID: 33845677734     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2006.10.027     Document Type: Article

References (13)

1. Tompa P. The interplay between structure and function in intrinsically unstructured proteins. FEBS Lett. 579 (2005) 3346-3354
2. Uversky V.N., Oldfield C.J., and Dunker A.K. Showing your ID: intrinsic disorder as an ID for recognition, regulation, and cell signaling. J. Mol. Recogn. 18 (2005) 343-384
3. Dyson H.J., and Wright P.E. Intrinsically unstructured proteins and their functions. Nat. Rev. Mol. Cell Biol. 6 (2005) 197-208
4. Dunker A.K., Lawson J.D., Brown C.J., Romero P., Oh J.S., Oldfield C.J., Campen A.M., Ratliff C.M., Hipps K.W., Ausio J., Nissen M.S., Reeves R., Kang C., Kissinger C.R., Bailey R.W., Griswold M.D., Chiu W., Garner E.C., and Obradovic Z. Intrinsically disordered protein. J. Mol. Graphics Modelling 19 (2001) 26-59
5. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72 (1976) 248-254
6. Smith P.K., Krohn R.I., Hermanson G.T., Mallia A.K., Gartner F.H., Provenzano M.D., Fujimoto E.K., Goeke N.M., Olson B.J., and Klenk D.C. Measurement of protein using bicinchoninic acid. Anal. Biochem. 150 (1985) 76-85
7. Lowry O.H., Rosebrough N.J., Farr A.L., and Randall R.J. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193 (1951) 265-275
8. Sedmak J.J., and Grossberg S.E. A rapid, sensitive, and versatile assay for protein using Coomassie Brilliant Blue G250. Anal. Biochem. 79 (1977) 544-552
9. Chan J.K., Thompson J.W., and Gill T.A. Quantitative determination of protamines by Coomassie Blue G assay. Anal. Biochem. 226 (1995) 191-193
10. Scopes R.K. Measurement of protein by spectrophotometry at 205 nm. Anal. Biochem. 59 (1974) 277-282
11. Sapan C.V., Lundblad R.L., and Price N.C. Colorimetric protein assay techniques. Biotechnol. Appl. Biochem. 29 (1999) 99-108
12. Stoscheck C.M. Quantitation of protein. Methods Enzymol. 182 (1990) 50-68
13. Compton S.J., and Jones C.G. Mechanism of dye response and interference in the Bradford protein assay. Anal. Biochem. 151 (1985) 369-374