Identification and serotyping of atypical Legionella pneumophila strains isolated from human and environmental sources

Journal of Applied Microbiology

Volumn 102, Issue 1, 2007, Pages 100-105

  Helbig, J H ^a   Benson, R F ^b   Pelaz, C ^c   Jacobs, E ^a   Luck P C ^a  

^a DRESDEN UNIVERSITY OF TECHNOLOGY   (Germany)

^b NATIONAL CENTER FOR EMERGING AND ZOONOTIC INFECTIOUS DISEASES   (United States)

^c INSTITUTO DE SALUD CARLOS III   (Spain)

Author keywords

Fatty acid analysis; Legionella pneumophila; mip gene; Mip protein; Monoclonal antibodies; Serotyping

Indexed keywords

ANTIGEN-ANTIBODY REACTIONS; EPITOPES; FATTY ACIDS; GENES; SURFACE PLASMON RESONANCE;

ANTIGENICS; ATYPICALS; FATTY ACID ANALYSE; IDENTIFICATION METHOD; LEGIONELLA PNEUMOPHILA; LEGIONELLAE; MIP GENE; MIP PROTEINS; SEROGROUPS; SEROTYPING;

MONOCLONAL ANTIBODIES;

MONOCLONAL ANTIBODY;

ANTIBODY; ANTIGEN; BACTERIUM; FATTY ACID; GENE; IDENTIFICATION METHOD; IMMUNOASSAY; PHENOTYPE; PROTEIN;

AGGLUTINATION TEST; ANALYTIC METHOD; ANTIGENICITY; ARTICLE; BACTERIAL STRAIN; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATION; BLOOD GROUP TYPING; CONTROLLED STUDY; CROSS REACTION; FATTY ACID ANALYSIS; GENE SEQUENCE; GENETIC VARIABILITY; GENOTYPE; IMMUNOBLOTTING; INTERMETHOD COMPARISON; LEGIONELLA PNEUMOPHILA; NONHUMAN; PHENOTYPE; SEROTYPING; VALIDATION PROCESS;

LEGIONELLA; LEGIONELLA PNEUMOPHILA;

EID: 33845666673     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2006.03057.x     Document Type: Article

References (15)

1. Brenner, D.J., Steigerwalt, A.G., Epple, P., Bibb, W.F., McKinney, R.M., Starnes, R.W., Colville, J.M., Selander, R.K. et al. (1988) Legionella pneumophila serogroup Lansing 3 isolated from a patient with fatal pneumonia, and descriptions of L. pneumophila subsp. pneumophila subsp. nov., L. pneumophila subsp. fraseri subsp. nov., and L. pneumophila subsp. pascullei subsp. nov. J Clin Microbiol 26, 1695-1703.
2. Cherry, W.B. and McKinney, R.M. (1979) Detection of Legionnaires' disease bacterium in clinical specimens by direct immunofluorescence. In "Legionnaires", the Disease, the Bacterium, and Methodology ed Jones, G.L. and Hebert, G.A. pp. 91-104. Atlanta, GA: US Department of Health, Education, and Welfare.
3. Fry, N.K., Alexiou-Daniel, S., Bangsborg, J.M., Bernander, S., Castellani Pastoris, M., Etienne, J., Forsblom, M., Gaia, V. et al. (1999) A multicenter evaluation of genotypic methods for the epidemiologic typing of Legionella pneumophila serogroup 1: results of a pan-European study. Clin Microbiol Infect 5, 462-477.
4. Gaia, V., Fry, N.K., Afshar, B., Lück, P.C., Meugnier, H., Etienne, J., Peduzzi, R. and Harrison, T.G. (2005) Consensus sequence-based scheme for epidemiological typing of clinical and environmental isolates of Legionella pneumophila. J Clin Microbiol 43, 2047-2052.
5. Helbig, J.H., Ludwig, B., Lück, P.C., Groh, A., Witzleb, W. and Hacker, J. (1995) Monoclonal antibodies to Legionella Mip proteins recognize genus- and species-specific epitopes. Clin Diagn Lab Immunol 2, 160-165.
6. Helbig, J.H., Kurtz, J.B., Castellani-Pastoris, M., Pelaz, C. and Lück, P.C. (1997) The antigenic lipopolysaccharide components of Legionella pneumophila recognized by monoclonal antibodies: possibilities and limitations for division into serogroups. J Clin Microbiol 35, 2841-2845.
7. Helbig, J.H., Bernander, S., Castellani Pastoris, M., Etienne, J., Gaia, V., Lauwers, S., Lindsay, D., Lück, P.C. et al. (2002). Pan-European study on Legionnaires' disease: distribution of Legionella pneumophila serogroups and monoclonal subgroups. Eur J Clin Microbiol Infect Dis 21, 710-716.
8. High, A.S., Torasian, S.T. and Rodgers, F.G. (1993) Cloning, nucleotide sequencing and expression in Escherichia coli of a gene (ompM) encoding a 25 kDa major outer-membrane protein (MOMP) of Legionella pneumophila. J Gen Microbiol 139, 1715-1721.
9. Joly, J.R., McKinney, R.M., Tobin, J.O., Bibb, W.F., Watkins, I.D. and Ramsay, D. (1986) Development of a standardized subgrouping scheme for Legionella pneumophila serogroup 1 using monoclonal antibodies. J Clin Microbiol 23, 768-771.
10. Joseph, C. (2004) Legionnaires' disease in Europe, 2000-2002. Epidemiol Infect 132, 417-424.
11. Lück, P.C. and Helbig, J.H. (2002) Typing of Legionella strains isolated from patients and environmental sources in Germany, 1990 to 2000. In Legionella ed. Marre, R. et al. pp. 267-270. Washington, DC: ASM Press.
12. Mayberry, W.R. (1984) Monohydroxy and dihydroxy fatty acid composition of Legionella species. Int J Syst Bacteriol 34, 321-326.
13. Pringler, N., Brydov, P. and Uldum, S.A. (2002) Occurrence of Legionella in Danish hot water systems. In Legionella ed. Marre, R. et al. pp. 298-301. Washington, DC: ASM Press.
14. Ratcliff, R.M., Lanser, J.A., Manning, P.A. and Heuzenroeder, M.W. (1998) Sequence-based classification scheme for the genus Legionella targeting the mip gene. J Clin Microbiol 36, 1560-1567.
15. Thacker, W., Plikaytis, B.B. and Wilkinson, H.W. (1985) Identification of 22 Legionella species and 33 serogroups with the slide agglutination test. J Clin Microbiol 21, 779-782.