Purification and characterization of lysozyme from purple washington clam Saxidomus purpurata

Fisheries Science

Volumn 72, Issue 6, 2006, Pages 1300-1305

  Miyauchi, Kouji ^a   Matsumiya, Masahiro ^a   Mochizuki, Atsushi ^a  

^a NIHON UNIVERSITY   (Japan)

Author keywords

Bivalve; Invertebrate; Lysozyme; N terminal amino acid; Purification; Saxidomus purpurata; Specific activity

Indexed keywords

BIVALVIA; INVERTEBRATA; MICROCOCCUS LUTEUS; SAXIDOMUS NUTTALLI; SAXIDOMUS PURPURATA;

EID: 33845581798     PISSN: 09199268     EISSN: 14442906     Source Type: Journal    
DOI: 10.1111/j.1444-2906.2006.01289.x     Document Type: Article

References (17)

1. Prager EM, Jolles P. Animal lysozymes c and g. In: Jolles P (ed.). An Overview, Lysozymes: Model Enzymes in Biochemistry and Biology. Birkhauser-Verlag, Basel. 1996; 9-31.
2. Jolles J, Jolles P. The lysozyme from Asterias rubens. Eur. J. Biochem. 1975; 54: 19-23.
3. Ito Y, Yoshikawa A, Hotani T, Fukuda S, Sugimura K, Imoto T. Amino acid sequences of lysozymes newly purified from invertebrates imply wide distribution of a novel class in the lysozyme family. Eur. J. Biochem. 1999; 259: 456-461.
4. Nilsen IW, Overbo K, Sandsdalen E, Sandaker E, Sletten K, Myrnes B. Protein purification and gene isolation of chlamysin, a cold-active lysozyme-like enzyme with antibacterial activity. FEBS Lett. 1999; 464: 153-158.
5. Bachali S, Jager M, Hassanin A, Schoentgen F, Jolls P, Fiala-Medioni A, Deutsch JS. Phylogenetic analysis of invertebrate lysozymes and the evolution of lysozyme function. J. Mol. Evol. 2002; 54: 652-664.
6. Bachali S, Bailly X, Jolls J, Jolls P, Deutsch J. The lysozyme of the starfish Asterias rubens. A paradigmatic type i lysozyme. Eur. J. Biochem. 2004; 271: 237-242.
7. McHenery JG, Birkbeck TH. Characterization of the lysozyme of Mytilus edulis (L.). Comp. Biochem. Physiol. 1982; 71B: 583-589.
8. Mochizuki A, Matsumiya M. Lysozyme activity in shellfish. Nippon Suisan Gakkaishi 1983; 49: 131-135 (in Japanese).
9. Mochizuki A, Matsumiya M. Properties of turbo lysozyme. Bull. Coll. Agr. Vet. Med. Nihon Univ. 1994; 51: 169-173 (in Japanese).
10. Takahashi K, Mori K, Nomura T. Existence and characteristics of lysozymes in marine bivalves. Nippon Suisan Gakkaishi 1986; 52: 863-868 (in Japanese).
11. Miyauchi K, Matsumiya M, Mochizuki A. Purification and characterization of lysozyme from brackishwater clam Corbicula japonica. Nippon Suisan Gakkaishi 2000; 66: 275-281 (in Japanese).
12. Miyauchi K, Matsumiya M, Mochizuki A. Distribution of lysozyme in the organs of shellfish. Nippon Suisan Gakkaishi 2003; 69: 211-213 (in Japanese).
13. Miura K, Matsumiya M, Mochizuki A. Seasonal variation in lysozyme activity in the mussel Mytilus edulis. Nippon Suisan Gakkaishi 1994; 60: 441-443 (in Japanese).
14. Ito Y, Nakamura M, Hotani T, Imoto T. Insect lysozyme from house fly (Musca domestica) larvae: possible digestive function based on sequence and enzymatic properties. J. Biochem. 1995; 118: 546-551.
15. Nanjo F, Sakai K, Usui T. p-Nitrophenyl penta-N-acetyl-β- chitopentaoside as a novel synthetic substrate for the colorimetric assay of lysozyme. J. Biochem. 1988; 104: 255-258.
16. Reisfeld RA, Lewis UJ, Williams DE. Disk electrophoresis of basic proteins and peptides on polyacrylamide gels. Nature 1962; 195: 281-283.
17. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the folin phenol reagent. J. Biol. Chem. 1951; 193: 265-275.