Proteolytic fragments of insulysin (IDE) retain substrate binding but lose allosteric regulation

Biochemistry

Volumn 45, Issue 50, 2006, Pages 15085-15091

  Song, Eun Suk ^a   Cady, Clint ^a   Fried, Michael G ^a   Hersh, Louis B ^a  

^a UNIVERSITY OF KENTUCKY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; BINDING ENERGY; CATALYST ACTIVITY; ENZYMES; REACTION KINETICS;

ALLOSTERIC KINETIC BEHAVIOR; PROTEINASE; PROTEOLYTIC FRAGMENTS OF INSULYSIN (IDE); VELOCITY CURVES;

ANTIBODIES;

ADENOSINE TRIPHOSPHATE; AMYLOID BETA PROTEIN[1-40]; BETA ENDORPHIN; CATION; FLUORESCENT DYE; INSULIN; INSULINASE; PEPTIDE; POLYHISTIDINE TAG; PROTEINASE K;

ALLOSTERISM; AMINO TERMINAL SEQUENCE; ARTICLE; BINDING AFFINITY; CARBOXY TERMINAL SEQUENCE; CHEMICAL BOND; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME BINDING; ENZYME KINETICS; ENZYME MECHANISM; ENZYME SUBSTRATE; ENZYME SUBSTRATE COMPLEX; NONHUMAN; NUCLEOTIDE BINDING SITE; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN FUNCTION; PROTEIN STABILITY; RAT; STRUCTURE ACTIVITY RELATION;

ALLOSTERIC REGULATION; AMYLOID BETA-PROTEIN; ANIMALS; BETA-ENDORPHIN; BINDING SITES; CATALYSIS; ENDOPEPTIDASE K; INSULIN; INSULYSIN; PEPTIDES; RATS; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY;

EID: 33845578299     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi061298u     Document Type: Article

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