Single scan TROSY and E.COSY suite of experiments for the measurement of residual dipolar couplings in proteins

Spectroscopy

Volumn 20, Issue 4, 2006, Pages 153-167

  Eliseo, Tommaso ^a   Gallo, Mariana ^a   Melis, Riccardo ^a   Paci, Maurizio ^a   Bazzo, Renzo ^b   Cicero, Daniel O ^a  

^a UNIVERSITY OF ROME TOR VERGATA   (Italy)

^b IRBM   (Italy)

Author keywords

eCOSY; NMR; Residual dipolar couplings; TROSY

Indexed keywords

MODIFICATION; MODULATION; SPECTROSCOPY; WATER;

ECOSY; PULSE FIELD GRADIENTS; RESIDUAL DIPOLAR COUPLINGS; TROSY;

PROTEINS;

CARBON 13; HYDROGEN; NITROGEN 15; PROTEIN;

ARTICLE; ARTIFACT REDUCTION; DIPOLE; ISOTOPE LABELING; MATHEMATICAL ANALYSIS; MOLECULAR INTERACTION; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; RESIDUAL DIPOLAR COUPLING;

EID: 33845432425     PISSN: 07124813     EISSN: None     Source Type: Journal    
DOI: 10.1155/2006/812107     Document Type: Article

References (25)

1. A. Bax and A. Grishaev, Weak alignment NMR: a hawk-eyed view of biomolecular structure, Curr. Opin. Struct. Biol. 15 (2005), 563-570.
2. A. Bax, G. Kontaxis and N. Tjandra, Dipolar couplings in macromolecular structure determination, Methods Enzymol. 339 (2001), 127-174.
3. F. Rodriguez-Castaneda, P. Haberz, A. Leonov and C. Griesinger, Paramagnetic tagging of diamagnetic proteins for solution NMR, Magn. Reson. Chem. 44 Spec. No (2006), S10-16.
4. J.H. Prestegard, K.L. Mayer, H. Valafar and G.C. Benison, Determination of protein backbone structures from residual dipolar couplings, Methods Enzymol. 394 (2005), 175-209.
5. J.A. Lukin, G. Kontaxis, V. Simplaceanu, Y. Yuan, A. Bax and C. Ho, Quaternary structure of hemoglobin in solution, Proc. Natl. Acad. Sci. USA 100 (2003), 517-520.
6. 1JCH splittings from magnetic-field dependence of J modulation in two-dimensional NMR spectra, J. Magn. Reson. 124 (1997), 512-515.
7. 15N-enriched human ubiquitin resulting from relaxation interference and residual dipolar coupling, J. Am. Chem. Soc. 118 (1996), 6264-6272.
8. M. Ottiger, F. Delaglio and A. Bax, Measurement of J and dipolar couplings from simplified two-dimensional NMR spectra, J. Magn. Reson. 131 (1998), 367-372.
9. P. Andersson, J.Weigelt and G. Otting, Spin-state selection filters fort he measurement of heteronuclear one-bond coupling constants, J. Biomol. NMR 12 (1998), 435-441.
10. B. Brutscher, Accurate measurement of small spin-spin couplings in partially aligned molecules using a novel J-mismatch compensated spin-state-selection filter, J. Magn. Reson. 151 (2001), 332-338.
11. 2 relaxation by mutual cancellation of dipole-dipole coupling and chemical shift anisotropy indicates an avenue to NMR structures of very large biological macromolecules in solution, Proc. Natl. Acad. Sci. USA 94 (1997), 12366-12371.
12. L. Kay, P. Keifer and T. Saarinen, Pure absorption gradient enhanced heteronuclear single quantum correlation spectroscopy with improved sensitivity, J. Am. Chem. Soc. 114 (1992) 10663-10665.
13. M. Czisch and R. Boelens, Sensitivity enhancement in the TROSY experiment, J. Magn. Reson. 134 (1998), 158-160.
14. J.Weigelt, Single scan, sensitivity- and gradient-enhanced TROSY for multidimensional NMR experiments, J. Am. Chem. Soc. 120 (1998), 10778-10779.
15. K.V. Pervushin, G. Wider and K. Wüthrich, Single transition-to-single transition polarization transfer (ST2-PT) in [15N,1H]-TROSY, J. Biomol. NMR 12 (1998), 345-348.
16. N couplings, J. Magn. Reson. 163 (2003), 208-214.
17. L. Ragona, M. Catalano, M. Luppi, D. Cicero, T. Eliseo, J. Foote, F. Fogolari, L. Zetta and H. Molinari, NMR dynamic studies suggest that allosteric activation regulates ligand binding in chicken liver bile acid binding protein, J. Biol. Chem. 281 (2006), 9697-9709.
18. M. Gallo, S. Melino, R. Melis, M. Paci and D.O. Cicero, Backbone NMR assignment of the 29.6 kDa Rhodanese protein from Azotobacter vinelandii, J. Biomol. NMR (2006), in press.
19. F. Delaglio, S. Grzesiek, G.W. Vuister, G. Zhu, J. Pfeifer and A. Bax, NMRPipe: a multidimensional spectral processing system based on UNIX pipes, J. Biomol. NMR 6 (1995) 277-293.
20. B.A. Johnson and R.A. Blevins, NMRView: A computer program for the visualization and analysis of NMR data, J. Biomol. NMR 4 (1994), 603-661.
21. M.R. Hansen, L. Mueller and A. Pardi, Tunable alignment of macromolecules by filamentous phage yields dipolar coupling interactions, Nat. Struct. Biol. 5 (1998), 1065-1074.
22. M. Rance, L.J. Patrick and A.G. Palmer III, Sensitivity improvement of transverse relaxation-optimized spectroscopy, J. Magn. Reson. 136 (1999), 92-101.
23. N-N residual dipolar couplings from 2D HN-N correlation spectra, J. Biomol. NMR 31 (2005), 35-47.
24. O.W. Sørensen, G.W. Eich, M.H. Levitt, G. Bodenhausen and R.R. Ernst, Product operator formalism for the description of NMR pulse experiments, Progress in NMR Spectroscopy 16 (1983), 163-192.
25. T. Schulte-Herbrüggen and O.W. Sørensen, Clean TROSY: compensation for relaxation-induced artifacts, J. Magn. Reson. 144 (2000), 123-128.