Detection of mutations in the β-globin gene by colorimetric staining of DNA microarrays visualized by a flatbed scanner

Analytical Biochemistry

Volumn 360, Issue 1, 2007, Pages 169-171

  Petersen, Jesper ^a   Stangegaard, Michael ^b   Birgens, Henrik ^b   Dufva, Martin ^a  

^a TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

^b UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

Agarose film; Colorimetry; DNA microarray; Genotyping; Monogenetic diseases

Indexed keywords

BIOCHIPS; GENES;

AGAROSE; DNA MICRO-ARRAY; FLATBED SCANNERS; GENOTYPING; GLOBIN GENE;

COLORIMETRY;

BETA GLOBIN;

5' UNTRANSLATED REGION; ARTICLE; COLORIMETRY; DNA MICROARRAY; DNA PROBE; FLUORESCENCE; GENE MUTATION; GENE SEQUENCE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROMOTER REGION; RNA HYBRIDIZATION; SINGLE NUCLEOTIDE POLYMORPHISM;

EID: 33845393763     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2006.08.014     Document Type: Article

References (11)

1. Dufva M., Petronis S., Bjerremann Jensen L., Krag C., and Christensen C. Characterization of an inexpensive, non-toxic, and highly sensitive microarray substrate. BioTechniques 37 (2004) 286-296
2. Koch C.A., Li P.C., and Utkhede R.S. Evaluation of thin films of agarose on glass for hybridization of DNA to identify plant pathogens with microarray technology. Anal. Biochem. 342 (2005) 93-102
3. 10 modified DNA probes immobilized on agarose films. Anal. Biochem. 352 (2006) 188-197
4. Zhong X.B., Reynolds R., Kidd J.R., Kidd K.K., Jenison R., Marlar R.A., and Ward D.C. Single-nucleotide polymorphism genotyping on optical thin-film biosensor chips. Proc. Natl. Acad. Sci. USA 100 (2003) 11559-11564
5. Chen J.J., Wu R., Yang P.C., Huang J.Y., Sher Y.P., Han M.H., Kao W.C., Lee P.J., Chiu T.F., Chang F., Chu Y.W., Wu C.W., and Peck K. Profiling expression patterns and isolating differentially expressed genes by cDNA microarray system with colorimetry detection. Genomics 51 (1998) 313-324
6. Bobrow M.N., Shaughnessy K.J., and Litt G.J. Catalyzed reporter deposition, a novel method of signal amplification: II. Application to membrane immunoassays. J. Immunol. Methods 137 (1991) 103-112
7. Taton T.A., Mirkin C.A., and Letsinger R.L. Scanometric DNA array detection with nanoparticle probes. Science 289 (2000) 1757-1760
8. Han A., Dufva M., Belleville E., and Christensen C. Detection of analyte binding to microarrays using gold nanoparticles labels and a desktop scanner. Lab. Chip. 3 (2003) 336-339
9. Alexandre I., Hamels S., Dufour S., Collet J., Zammatteo N., De Longueville F., Gala J.L., and Remacle J. Colorimetric silver detection of DNA microarrays. Anal. Biochem. 295 (2001) 1-8
10. Afanassiev V., Hanemann V., and Wölfl S. Preparation of DNA and protein micro arrays on glass slides coated with an agarose film. Nucleic Acids Res. 28 (2000) e66
11. Belleville E., Dufva M., Aamand J., Bruun L., and Christensen C. Quantitative assessment of factors affecting the sensitivity of a competitive immuno-microarray for pesticide detection. BioTechniques 35 (2003) 1044-1051