Genetic characterization of the dihydrofolate reductase gene of Pneumocystis jirovecii isolates from Portugal

Journal of Antimicrobial Chemotherapy

Volumn 58, Issue 6, 2006, Pages 1246-1249

  Costa, Marina C ^a   Esteves, Francisco ^a   Antunes, Francisco ^b   Matos, Olga ^a  

^a INSTITUTO DE HIGIENE E MEDICINA TROPICAL   (Portugal)

^b HOSPITAL DE SANTA MARIA   (Portugal)

Author keywords

Co trimoxazole; Drug resistance; Mutations; Polymorphisms

Indexed keywords

ANTIFUNGAL AGENT; COTRIMOXAZOLE; DIHYDROFOLATE REDUCTASE; PENTAMIDINE;

AMINO ACID SUBSTITUTION; ARTICLE; DRUG RESISTANCE; FUNGAL GENETICS; FUNGUS MUTATION; GENE MUTATION; GENE SEQUENCE; GENETIC IDENTIFICATION; GENETIC POLYMORPHISM; GENETIC VARIABILITY; GENOTYPE; HUMAN; IMMUNE DEFICIENCY; LUNG MYCOSIS; MAJOR CLINICAL STUDY; NONHUMAN; NUCLEIC ACID BASE SUBSTITUTION; NUCLEOTIDE SEQUENCE; PNEUMOCYSTIS JIROVECI; POINT MUTATION; POLYMERASE CHAIN REACTION; PORTUGAL;

AMINO ACID SUBSTITUTION; DNA, FUNGAL; GENOTYPE; HUMANS; IMMUNOCOMPROMISED HOST; MOLECULAR SEQUENCE DATA; MUTATION; PNEUMOCYSTIS JIROVECI; PNEUMONIA, PNEUMOCYSTIS; POINT MUTATION; POLYMERASE CHAIN REACTION; POLYMORPHISM, GENETIC; PORTUGAL; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID; TETRAHYDROFOLATE DEHYDROGENASE;

EID: 33845349672     PISSN: 03057453     EISSN: 14602091     Source Type: Journal    
DOI: 10.1093/jac/dkl411     Document Type: Article

References (12)

1. Nahimana A, Rabodonirina M, Zanetti G et al. Association between a specific Pneumocystis jiroveci dihydropteroate synthase mutation and failure of pyrimethamine/sulfadoxine prophylaxis in human immunodeficiency virus-positive and -negative patients. J Infect Dis 2003; 188: 1017-23.
2. Iliades P, Meshnick SR, Macreadie IG. Analysis of Pneumocystis jirovecii DHPS alleles implicated in sulfamethoxazole resistance using an Escherichia coli model system. Microb Drug Resist 2005; 11: 1-8.
3. Khalil I, Ronn AM, Alifrangis M et al. Dihydrofolate reductase and dihydropteroate synthase genotypes associated with in vitro resistance of Plasmodium falciparum to pyrimethamine, trimethoprim, sulfadoxine, and sulfamethoxazole. Am J Trop Med Hyg 2003; 68: 586-9.
4. Dale GE, Broger C, D'Arcy A et al. A single amino acid substitution in Staphylococcus aureus dihydrofolate reductase determines trimethoprim resistance. J Mol Biol 1997; 266: 23-30.
5. Maskell JP, Sefton AM, Hall LM et al. Multiple mutations modulate the function of dihydrofolate reductase in trimethoprim-resistant Streptococcus pneumoniae. Antimicrob Agents Chemother 2001; 45: 1104-8.
6. Takahashi T, Endo T, Nakamura T et al. Dihydrofolate reductase gene polymorphisms in Pneumocystis carinii f. sp. hominis in Japan. J Med Microbiol 2002; 51: 510-5.
7. Nahimana A, Rabodonirina M, Bille J et al. Mutations of Pneumocystis jirovecii dihydrofolate reductase associated with failure of prophylaxis. Antimicrob Agents Chemother 2004; 48: 4301-5.
8. Robberts FJ, Chalkley LJ, Weyer K et al. Dihydropteroate synthase and novel dihydrofolate reductase gene mutations in strains of Pneumocystis jirovecii from South Africa. J Clin Microbiol 2005; 43: 1443-4.
9. Matos O, Lundgren B, Caldeira L et al. Evaluation of two nested polymerase chain reactions for diagnosis of Pneumocystis carinii pneumonia in immunocompromised patients. Clin Microbiol Infect 2000; 6: 149-51.
10. Costa MC, Gaspar J, Mansinho K et al. Detection of Pneumocystis jirovecii dihydropteroate synthase polymorphisms in patients with Pneumocystis pneumonia. Scand J Infect Dis 2005; 37: 766-71.
11. Ma L, Borio L, Masur H et al. Pneumocystis carinii dihydropteroate synthase but not dihydrofolate reductase gone mutations correlate with prior trimethoprim-sulfamethoxazole or dapsone use. J Infect Dis 1999; 180: 1969-78.
12. Ma L, Kovacs JA. Expression and characterization of recombinant human-derived Pneumocystis carinii dihydrofolate reductase. Antimicrob Agents Chemother 2000; 44: 3092-6.