A method for the determination of nitrification rates in oligotrophic marine seawater by gas chromatography/mass spectrometry

Marine Chemistry

Volumn 103, Issue 1-2, 2007, Pages 84-96

  Clark, Darren R ^a   Rees, Andrew P ^a   Joint, Ian ^a  

^a PLYMOUTH MARINE LABORATORY   (United Kingdom)

Author keywords

15N; AMT; f ratio; GC MS; Nitrification; North east Atlantic

Indexed keywords

AMMONIUM; GAS CHROMATOGRAPHY; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; NITRATE; NITRIFICATION; NITRITE; OLIGOTROPHIC ENVIRONMENT; OXIDATION; SEAWATER;

EID: 33845341417     PISSN: 03044203     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.marchem.2006.06.005     Document Type: Article

References (45)

1. Allen A.E., Howard-Jones M.H., Booth M.G., Frischer M.E., Verity P.G., Bronk D.A., and Sanderson M.P. Importance of heterotrophic bacterial assimilation of ammonium and nitrate in the Barents Sea during summer. J. Mar. Syst. 38 (2002) 93-108
2. Ålvarez-Salgado X.A., and Gilcotc M. Inferring nitrification rates with an inverse method in a coastal upwelling system, Ría de Vigo (NW Spain). Mar. Ecol. Prog. Ser. 276 (2004) 3-17
3. An S., and Joye S.B. Enhancement of coupled nitrification-denitrification by benthic photosynthesis in shallow estuarine sediments. Limnol. Oceanogr. 46 (2001) 62-74
4. Bianchi M., Bonin P., and Feliatra F. Bacterial nitrification and denitrification rates in the Rhône River plume (Northwestern Mediterranean Sea). Mar. Ecol. Prog. Ser. 103 (1994) 197-202
5. de Bie M.J.M., Starink M., Boschker H.T.S., Peene J.J., and Laanbroek H.J. Nitrification in the Schelde estuary: methodological aspects and factors influencing its activity. FEMS Microbiol. Ecol. 42 (2002) 99-107
6. Billen G. Evaluation of nitrifying activity in sediments by dark C-14 bicarbonate incorporation. Water Res. 10 (1976) 51-57
7. + dilution technique. Appl. Environ. Microbiol. 37 (1979) 760-765
8. Caffrey J.M., Harrington N., Solem I., and Ward B.B. Biogeochemical processes in a small California estuary. 2. Nitrification activity, community structure and role in nitrogen budgets. Mar. Ecol. Prog. Ser. 248 (2003) 27-40
9. 15N isotope dilution technique. Mar. Biol. 54 (1979) 33-40
10. Carlucci A.F., and Strickland J.D.H. The isolation, purification and some kinetic studies of marine nitrifying bacteria. J. Exp. Mar. Biol. Ecol. 2 (1968) 156-166
11. Clark D.R., Flynn K.J., and Owens N.J.P. The large capacity for dark-nitrate-assimilation in diatoms may overcome nitrate limitation of growth. New Phytol. 155 (2002) 101-108
12. Clark D.R., Fileman T.W., and Joint I. Determination of ammonium regeneration rates in the oligotrophic ocean determined by gas chromatography/mass spectrometry. Mar. Chem. 98 (2006) 121-130
13. Diaz F., and Raimbault P. Nitrogen regeneration and dissolved organic nitrogen release during spring in a NW Mediterranean coastal zone (Gulf of Lions): implication for the estimation of new production. Mar. Ecol. Prog. Ser. 197 (2000) 51-65
14. Dore J.E., and Karl D.M. Nitrification in the euphotic zone as a source for nitrite, nitrate and nitrous oxide at Station ALOHA. Limnol. Oceanogr. 41 (1996) 1619-1628
15. Dugdale R.C., and Goering J.J. Uptake of new and regenerated forms of nitrogen in primary productivity. Limnol. Oceanogr. 12 (1967) 196-206
16. Eppley R.W., and Peterson B.J. Particulate organic matter flux and planktonic new production in the deep ocean. Nature 282 (1979) 677-680
17. Eppley R.W., and Renger E.H. Nitrate-based primary production in nutrient depleted surface waters off California. Oceanography 24 (1986) 495-509
18. Eppley R.W., Garside C., Renger E.H., and Orellana E. Variability of nitrate concentration in nitrogen-depleted surface waters. Mar. Biol. 107 (1990) 53-60
19. Feliatra F., and Bianchi M. Rates of nitrification and carbon uptake in the Rhône River plume (Northwestern Mediterranean Sea). Microb. Ecol. 26 (1993) 21-28
20. Fernández C., Raimbault P., Garcia N., and Rimmelin P. An estimation of annual new production and carbon fluxes in the northeast Atlantic Ocean during 2001. J. Geophys. Res. 110 (2005) C07S13 10.1029/2004JC002616
21. Flynn K.J., Clark D.R., and Owens N.J.P. Modelling suggests that optimization of dark nitrogen-assimilation need not be a critical selective feature in phytoplankton. New Phytol. 155 (2002) 109-119
22. Glibert P.M., Lipschultz F., McCarthy J.J., and Altabet M.A. Isotope dilution models of uptake and remineralisation of ammonium by phytoplankton. Limnol. Oceanogr. 27 (1982) 639-650
23. Glover H.E. The relationship between inorganic nitrogen oxidation and organic carbon production in batch and chemostat cultures of marine nitrifying bacteria. Arch. Microbiol. 74 (1985) 295-300
24. Grob K., Grob G., and Grob Jr. K. Testing capillary gas chromatography columns. J. Chromatogr. 219 (1981) 13-20
25. Harrison W.G., Platt T., and Lewis M.R. f-ratio and its relationship to ambient nitrate concentration in coastal waters. J. Plankton Res. 9 (1987) 235-248
26. Harrison W.G., Harris L.R., and Irwin B.D. The kinetics of nitrogen utilisation in the oceanic mixed layer: nitrate and ammonium interactions at nanomolar concentrations. Limnol. Oceanogr. 41 (1996) 16-32
27. IOC, Paris (France). Protocols for the Joint Global Flux Study (JGOFS) core measurements. Manuals Guides vol. 29 (1994) 126 pp.
28. Joint I., Rees A.P., and Woodward E.M.S. Primary production and nutrient assimilation in the Iberian upwelling in August 1998. Prog. Oceanogr. 51 (2001) 303-320
29. 15N. Limnol. Oceanogr. 29 (1984) 379-386
30. Lipschultz F. A time-series assessment of the nitrogen cycle at BATS. Deep Sea Res. II 48 (2001) 1897-1924
31. Lipschultz F., Wofsy S.C., and Fox L.E. Nitrogen metabolism of the eutrophic Delaware River ecosystem. Limnol. Oceanogr. 31 (1986) 701-716
32. 15N tracer studies of the primary nitrite maximum. J. Mar. Res. 39 (1981) 203-226
33. Preston T., Bury S., Presing M., Moncoiffe G., and Slater C. Isotope dilution analysis of combined nitrogen in natural waters: I. Ammonium. Rapid Commun. Mass Spectrom. 10 (1996) 959-964
34. Preston T., Zainal K., Anderson S., Bury S.J., and Slater C. Isotope dilution analysis of combined nitrogen in natural waters: III. Nitrate and nitrite. Rapid Commun. Mass Spectrom. 12 (1998) 423-428
35. Raimbault P., Slawyk G., Boudjellal B., Coatanoan C., Conan P., Coste B., Garcia N., Moutin T., and Pujo-Pay M. Carbon and nitrogen uptake and export in the equatorial Pacific at 150°W: evidence for an efficient regenerated production cycle. J. Geophys. Res. 104 (1999) 3341-3356
36. Rees A.P., Woodward E.M.S., Robinson C., Cummings D.G., Tarran G.A., and Joint I. Size-fractionated nitrogen uptake and carbon fixation during a developing coccolithophore bloom in the north Sea during June 1999. Deep-Sea Res. 49 (2002) 2905-2927
37. Rees, A.P., Woodward, E.M.S., Joint, I., (in press). Concentrations and uptake of nitrate and ammonium in the Atlantic Ocean between 60°N and 50°S. Deep-Sea Res.
38. Sweeney R.E., Liu K.K., and Kaplan I.R. Oceanic nitrogen isotopes and their uses in determining the source of sedimentary nitrogen. N. Z. Depart. Sci. Ind. Res. Bull. 220 (1978) 9-26
39. Varela M.M., Bode A., Fernández E., Gónzalez N., Kitidis V., Varela M., and Woodward E.M.S. Nitrogen uptake and dissolved organic nitrogen release in planktonic communities characterised by phytoplankton size-structure in the Central Atlantic Ocean. Deep-Sea Res. I 52 (2005) 1637-1661
40. Ward B.B. Nitrification in marine environments. In: Prosser J.I. (Ed). Nitrification. Special publications of the Society for General Microbiology vol. 20 (1986), IRL Press, Oxford
41. Ward B.B. Nitrogen transformations in the Southern Californian Bight. Deep-Sea Res. 34 (1987) 785-805
42. Ward B.B., Olson R.J., and Perry M.J. Microbial nitrification rates in the primary nitrite maximum off Southern California. Deep-Sea Res. 29 (1982) 913-918
43. Ward B.B., Talbot M.C., and Perry M.J. Contributions of phytoplankton and nitrifying bacteria to ammonium and nitrite dynamics in coastal waters. Cont. Shelf Res. 3 (1984) 383-398
44. Ward B.B., Glover H.E., and Lipschultz F. Chemoautotrophic activity and nitrification in the oxygen minimum zone off Peru. Deep-Sea Res. 36 (1989) 1031-1051
45. Ward B.B., Kilpatrick K.A., Renger E.H., and Eppley R.W. Biological cycling in the nitricline. Limnol. Oceanogr. 34 (1989) 493-513