The antioxidant role of a reagent, 2′,7′- dichlorodihydrofluorescin diacetate, detecting reactive-oxygen species and blocking the induction of heme oxygenase-1 and preventing cytotoxicity

Journal of Biochemistry

Volumn 140, Issue 4, 2006, Pages 483-489

  Andoh, Yoshihiro ^a   Mizutani, Atsushi ^a   Ohashi, Tomoko ^a   Kojo, Shosuke ^b   Ishii, Tetsuro ^c   Adachi, Yasushi ^d   Ikehara, Susumu ^d   Taketani, Shigeru ^a  

^a KYOTO INSTITUTE OF TECHNOLOGY   (Japan)

^b NARA WOMEN'S UNIVERSITY   (Japan)

^c UNIVERSITY OF TSUKUBA   (Japan)

^d KANSAI MEDICAL UNIVERSITY   (Japan)

Author keywords

2 ,7 dichlorodihydrofluorescin; Cytotoxicity; Heme oxygenase 1; Nrf2; ROS

Indexed keywords

ANTIOXIDANT; ARSENIC TRIOXIDE; CADMIUM; DICHLORODIHYDROFLUORESCEIN DIACETATE; HEME OXYGENASE 1; HEMIN; MITOGEN ACTIVATED PROTEIN KINASE; REACTIVE OXYGEN METABOLITE;

ANTIOXIDANT ACTIVITY; ARTICLE; CYTOTOXICITY; ENZYME ACTIVITY; ENZYME INDUCTION; ENZYME INHIBITION; ENZYME PHOSPHORYLATION; GENETIC TRANSCRIPTION; HUMAN; HUMAN CELL; OXIDATIVE STRESS; PROTEIN EXPRESSION;

ACETYLCYSTEINE; ACTIVE TRANSPORT, CELL NUCLEUS; ANTIOXIDANTS; ARSENITES; CADMIUM; CELL NUCLEUS; CELL SURVIVAL; CYTOPROTECTION; ENZYME INDUCTION; EXTRACELLULAR SIGNAL-REGULATED MAP KINASES; FLUORESCEINS; HELA CELLS; HEME OXYGENASE-1; HEMIN; HUMANS; NF-E2-RELATED FACTOR 2; OXIDATIVE STRESS; PHOSPHORYLATION; PROMOTER REGIONS (GENETICS); REACTIVE OXYGEN SPECIES;

EID: 33845337024     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/jb/mvj187     Document Type: Article

References (38)

1. Maines, M.D. (1997) The heme oxygenase system: a regulator of second messenger gases. Ann. Rev. Pharmacol. Toxicol. 37, 517-554
2. Shibahara, S. (1994) Heme oxygenase - Regulation of and physiological impication in heme catabolism. In Regulation of Heme Protein Synthesis (Fujita, H., ed.) pp. 103-116, AlphaMed Press, Ohio
3. Shibahara, S., Kitamuro, T., and Takahashi, K. (2002) Heme degradation and human disease: diversity is the soul of life. Antioxid. Redox Signal. 4, 593-602
4. Hayashi, S., Omata, Y., Sakamoto, H., Higashimoto, Y., Hara, T., Sagara, Y., and Noguchi, M. (2004) Characterization of rat heme oxygenase-3 gene. Implication of processed pseudogenes derived from heme oxygenase-2 gene. Gene 336, 241-250
5. Taketani, S., Kohno, H., Yoshinaga, T., and Tokunaga, R. (1989) The human 32-kDa stress protein induced by exposure to arsenite and cadmium ions is heme oxygenase. FEBS Lett. 245, 173-176
6. Ryter, S.W. and Ryrrell, R.M. (2001) The heme synthesis and degradation pathways: role in oxidant sensitivity. Heme oxygenase has both pro- and antioxidant properties. Free Rad. Biol. Med. 28, 289-309
7. Taketani, S., Sato, H., Yoshinaga, T., Tokunaga, R., Ishii, T., and Bannai, S. (1990) Induction in mouse peritoneal macrophages of 34 kDa stress protein and heme oxygenase by sulfhydryl-reactive agents. J. Biochem. 108, 28-32
8. Rensing, H., Bauer, I., Peters, I., Wein, T., Silomon, M., Jaeschke, H., and Bauer, M. (1999) Role of reactive oxygen species for hepatocellular injury and heme oxygenase-1 gene expression after hemorrhage and resuscitation. Shock 12, 300-308
9. Yachie, A., Niida, Y., Wada, T., Igarashi, N., Kaneda, H., Toma, T., Ohta, K., Kasahara, Y., and Koizumi, S. (1999) Oxidative stress causes enhanced endothelial cell injury in human heme oxygenase-1 deficiency. J. Clin. Invest. 103, 129-135
10. Kapturczak, M.H., Wasserfall, C., Brusko, T., Campbell-Thompson, M., Ellis, T.M., Atkinson, M.A., and Agarwal, A. (2004) Heme oxygenase-1 modulates early inflammatory responses: evidence from the heme oxygenase-1-deficient mouse. Am. J. Path. 165, 1045-1053
11. Ohashi, T., Kakimoto, K., Sokawa, Y., and Taketani, S. (2002) Semi-quantitative estimation of heme/hemoprotein with dichlorodihydrofluorescin diacetate. Anal. Biochem. 30, 392-395
12. Ohashi, T., Mizutani, A., Murakami, A., Kojo, S., Ishii, T., and Taketani, S. (2002) Rapid oxidation of dichlorodihydrofluorescin with heme and hemoproteins: formation of the fluorescein is independent of the generation of reactive oxygen species. FEBS Lett. 511, 21-27
13. Andoh, Y., Suzuki, H., Araki, M., Mizutani, A., Ohashi, T., Okumura, T., Adachi, Y., Ikehara, S., and Taketani, S. (2004) Low- and high-level expressions of heme oxygenase-1 in cultured cells under uninduced conditions. Biochem. Biophys. Res. Commun. 320, 722-729
14. Ishii, T., Itoh, K., Takahashi, S., Sato, H., Yanagawa, T., Katoh, Y., Bannai, S., and Yamamoto, M. (2000) Transcription factor Nrf2 coordinately regulates a group of oxidative stress-inducible genes in macrophages. J. Biol. Chem. 275, 16023-16029
15. Shibahara, S., Yoshizawa, M., Suzuki, H., Takeda, K., Meguro, K., and Endo, K. (1993) Functional analysis of cDNAs for two types of human heme oxygenase and evidence for their separate regulation. J. Biochem. 113, 214-218
16. Tahara, T., Sun, J., IgarashI, K., and Taketani, S. (2004) Heme-dependent up-regulation of the alpha-globin gene expression by transcriptional repressor Bach1 in erythroid cells. Biochem. Biophys. Res. Commun. 324, 77-85
17. Chang, S.H., Garcia, J., Melendez, J.A., Kilberg, M.S., and Agarwal, A. (2003) Haem oxygenase 1 gene induction by glucose deprivation is mediated by reactive oxygen species via the mitochondrial electron-transport chain. Biochem. J. 371, 877-885
18. Alam, J. (1994) Multiple elements within the 5′ distal enhancer of the mouse heme oxygenase-1 gene mediate induction by heavy metals. J. Biol. Chem. 269, 25049-52056
19. Motohashi, H., Shavit, J.A., Igarashi, K., Yamamoto, M., and Engel, J.D. (1996) The world according to Maf. Nucleic Acids Res. 25, 2953-2959
20. Masuya, Y., Kameshita, I., Fujisawa, H., Kohno, H., Hioki, K., Tokunaga, R., and Taketani, S. (1999) MAP kinase-independent induction of proto-oncogene c-fos mRNA by hemin in human cells. Biochem. Biophys. Res. Commun. 260, 289-295
21. Zipper, L.M. and Mulcahy, R.T. (2003) Erk activation is required for Nrf2 nuclear localization during pyrrolidine dithiocarbamate induction of glutamate cysteine ligase modulatory gene expression in HepG2 cells. Toxicol. Sci. 73, 124-134
22. Rota, C., Chignell, C.F., and Mason, R.P. (1999) Evidence for free radical formation during the oxidation of 2′-7′-dichlorofluorescin to the fluorescent dye 2′-7′-dichlorofluorescein by horseradish peroxidase: possible implications for oxidative stress measurements. Free Radic. Biol. Med. 27, 873-881
23. Rota, C., Fann, Y.C., and Mason, R.P. (1999) Phenoxyl free radical formation during the oxidation of the fluorescent dye 2′,7′- dichlorofluorescein by horseradish peroxidase. Possible consequences for oxidative stress measurements. J. Biol. Chem. 274, 28161-28168
24. Balla, J., Jacob, H.S., Balla, G., Nath, K., Eaton, J.W., and Vercellotti, G.M. (1993) Endothelial-cell heme uptake from heme proteins: induction of sensitization and desensitization to oxidant damage. Proc. Natl. Acad. Sci. USA 90, 9285-9289
25. Gonzalez-Michaca, L., Farrugia, G., A.J. Croatt, A.J. Alam, J., and Nath, K.A. (2004) Heme: a determinant of life and death in renal tubular epithelial cells. Am. J. Physiol. Renal Physiol. 286, F370-F377
26. Aft, R.L. and Mueller, G.C. (1984) Hemin-mediated oxidative degradation of proteins. J. Biol. Chem. 259, 301-305
27. Gutteridge, J.M. and Smith,A. (1988) Antioxidant protection by haemopexin of haem-stimulated lipid peroxidation. Biochem. J. 256, 861-865
28. Suliman, H.B., Carraway, M.S., Velsor, L.W., Day, B.J., Ghio, A.J., and Piantadosi, C.A. (2002) Rapid mtDNA deletion by oxidants in rat liver mitochondria after hemin exposure. Free Radic. Biol. Med. 32, 246-256
29. Kyriakis, J.M. and Avruch, J. (2001) Mammalian mitogen-activated protein kinase signal transduction pathways activated by stress and inflammation. Physiol. Rev. 81, 807-869
30. Iles, K.E., Dickinson, D.A., Wigley, A.F., Welty, N.E., Blank, V., and Forman, H.J. (2005) HNE increases HO-1 through activation of the ERK pathway in pulmonary epithelial cells. Free Radic. Biol. Med. 39, 355-364
31. 2-induced cell death. Eur. J. Pharmacol. 483, 63-173
32. Chuang, S.M., Wang, I.C., HwuaY.S., and Yang, J.L. (2003) Short-term depletion of catalase suppresses cadmium-elicited c-Jun N-terminal kinase activation and apoptosis: role of protein phosphatases. Carcinogenesis 24, 7-15
33. Galan, A., Garcia-Bermejo, M.L., Troyano, A., Vilaboa, N.E., de Blas, E., Kazanietz, M.G., and Aller, P. (2003) Stimulation of p38 mitogen-activated protein kinase is an early regulatory event for the cadmium-induced apoptosis in human promonocytic cells. J. Biol. Chem. 275, 11418-11424
34. Cao, J., Semenova, M.M., Solovyan, V.T., Han, J., Coffey, E.T., and Courtney,M.J. (2004) Distinct requirements for p38alpha and c-Jun N-terminal kinase stress-activated protein kinases in different forms of apoptotic neuronal death. J. Biol. Chem. 279, 35903-35913
35. Kim, S.D., Moon, C.K., Eun, S.Y., Ryu, P.D., and Jo, S.A. (2005) Identification of ASK1, MKK4, JNK, c-Jun, and caspase-3 as a signaling cascade involved in cadmium-induced neuronal cell apoptosis. Biochem. Biophys. Res. Commun. 328, 326-334
36. Masuya, Y., Hioki, K., Tokunaga, R., and Taketani, S. (1999) Involvement of the tyrosine phosphorylation pathway in induction of human heme oxygenase-1 by hemin, sodium arsenite, and cadmium chloride. J. Biochem. 124, 628-633
37. 2 but not tumor necrosis factor. J. Biol. Chem. 280, 19036-19044
38. Gao, X., Dinkova-Kostova, A.T., and Talalay, P. (2001) Powerful and prolonged protection of human retinal pigment epithelial cells, keratinocytes, and mouse leukemia cells against oxidative damage: the indirect antioxidant effects of sulforaphane. Proc. Natl. Acad. Sci. USA 98, 15221-15226