Antisense oligonucleotide against the Ca channel β subunit decreases L-type Ca current in rat ventricular myocytes

Biochemical and Biophysical Research Communications

Volumn 352, Issue 3, 2007, Pages 794-798

  Leach, Rob N ^a   Brette, Fabien ^b   Orchard, Clive H ^c  

^a UNIVERSITY OF LEEDS   (United Kingdom)

^b UNIVERSITY OF MANCHESTER   (United Kingdom)

^c UNIVERSITY OF BRISTOL   (United Kingdom)

Author keywords

Subunit; Calcium current; Cardiac myocytes; L type Ca channel

Indexed keywords

ANTISENSE OLIGONUCLEOTIDE; CALCIUM CHANNEL L TYPE; OLIGONUCLEOTIDE;

ALPHA CHAIN; AMPLITUDE MODULATION; ANIMAL CELL; ANIMAL CELL CULTURE; ANTIBODY LABELING; ARTICLE; BETA CHAIN; CALCIUM CURRENT; CELL ACTIVATION; CELL ISOLATION; CELL MEMBRANE; CONTROLLED STUDY; HEART MUSCLE CELL; HEART VENTRICLE WALL; NONHUMAN; PATCH CLAMP; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; RAT; STAINING; WHOLE CELL;

ANIMALS; CALCIUM; CALCIUM CHANNELS, L-TYPE; CALCIUM SIGNALING; CELLS, CULTURED; GENE SILENCING; HEART VENTRICLES; MALE; MEMBRANE POTENTIALS; MYOCYTES, CARDIAC; OLIGONUCLEOTIDES, ANTISENSE; RATS; RATS, WISTAR;

EID: 33845293759     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2006.11.099     Document Type: Article

References (19)

1. Beuckelmann D.J., and Wier W.G. Mechanism of release of calcium from sarcoplasmic reticulum of guinea-pig cardiac cells. J. Physiol. 405 (1988) 233-255
2. Bers D.M. Excitation-contraction Coupling and Cardiac Contractile Force. second ed. (2001), Kluwer Academic, Dordrecht, Netherlands
3. 1C and β subunits generate the necessary signal for membrane targeting of class C L-type calcium channels. J. Biol. Chem. 274 (1999) 2137-2144
4. 2+ channel beta subunits revealed by their expression in adult rat heart cells. J. Physiol. 541 (2002) 435-452
5. Serikov V., Bodi I., Koch S.E., Muth J.N., Mikala G., Martinov S.G., Haase H., and Schwartz A. Mice with cardiac-specific sequestration of the beta-subunit of the L-type calcium channel. Biochem. Biophys. Res. Commun. 293 (2002) 1405-1411
6. Takahashi S.X., Miriyala J., and Colecraft H.M. Membrane-associated guanylate kinase-like properties of beta-subunits required for modulation of voltage-dependent Ca2+ channels. Proc. Natl. Acad. Sci. USA 101 (2004) 7193-7198
7. Yasuda T., Chen L., Barr W., McRory J.E., Lewis R.J., Adams D.J., and Zamponi G.W. Auxiliary subunit regulation of high-voltage activated calcium channels expressed in mammalian cells. Eur.J. Neurosci. 20 (2004) 1-13
8. Canti C., Davies A., Berrow N.S., Butcher A.J., Page K.M., and Dolphin A.C. Evidence for two concentration-dependent processes for beta-subunit effects on alpha1B calcium channels. Biophys. J. 81 (2001) 1439-1451
9. Chu P.J., Larsen J.K., Chen C.C., and Best P.M. Distribution and relative expression levels of calcium channel beta subunits within the chambers of the rat heart. J. Mol. Cell Cardiol. 36 (2004) 423-434
10. Foell J.D., Balijepalli R.C., Delisle B.P., Yunker A.M., Robia S.L., Walker J.W., McEnery M.W., January C.T., and Kamp T.J. Molecular heterogeneity of calcium channel beta-subunits in canine and human heart: evidence for differential subcellular localization. Physiol. Genomics 17 (2004) 183-200
11. Brette F., Komukai K., and Orchard C.H. Validation of formamide as a detubulation agent in isolated rat cardiac cells. Am. J. Physiol. 283 (2002) H1720-H1728
12. Mitcheson S., Hancox J.C., and Levi A.J. Cultured adult cardiac myocytes: future applications, culture methods, morphological and electrophysiological properties. Cardiovasc. Res. 39 (1998) 280-300
13. Berrow N.S., Campbell V., Fitzgerald E.M., Brickley K., and Dolphin A.C. Antisense depletion of beta-subunits modulates the biophysical and pharmacological properties of neuronal calcium channels. J. Physiol. 482 (1995) 481-491
14. Balijepalli R.C., Lokuta A.J., Maertz N.A., Buck J.M., Haworth R.A., Valdivia H.H., and Kamp T.J. Depletion of T-tubules and specific subcellular changes in sarcolemmal proteins in tachycardia-induced heart failure. Cardiovasc. Res. 59 (2003) 67-77
15. Brette F., Salle L., and Orchard C.H. Differential modulation of L-type Ca2+ current by SR Ca2+ release at the T-tubules and surface membrane of rat ventricular myocytes. Circ. Res. 95 (2004) e1-e7
16. Nascimento J.H., Salle L., Hoebeke J., Argibay J., and Peineau N. cGMP-mediated inhibition of cardiac L-type Ca2+ current by a monoclonal antibody against the M2 ACh receptor. Am. J. Physiol. Cell Physiol. 281 (2001) C1251-C1258
17. Brette F., and Orchard C.H. T-tubule function in mammalian cardiac myocytes. Circ. Res. 92 (2003) 1182-1192
18. Sha Q., Robinson S.W., McCulle S.L., Shorofsky S.R., Welling P.A., Goldman L., and Balke C.W. An antisense oligonucleotide against H1 inhibits the classical sodium current but not ICaTTX in rat ventricular cells. J. Physiol. 547 (2003) 435-440
19. Bichet D., Cornet V., Geib S., Carlier E., Volsen S., Hoshi T., Mori Y., and De Waard M. The I-II loop of the Ca2+ channel alpha1 subunit contains an endoplasmic reticulum retention signal antagonized by the beta subunit. Neuron 25 (2000) 177-190