Protein isolate from the herb Phyllanthus niruri modulates carbon tetrachloride-induced cytotoxicity in hepatocytes

Toxicology Mechanisms and Methods

Volumn 17, Issue 1, 2006, Pages 41-47

  Bhattacharjee, Rajesh ^a   Sil, Parames C ^a  

^a BOSE INSTITUTE   (India)

Author keywords

Antioxidant; CCl4 Induced Cytototoxicity; Hepatoprotection; Oxidative Stress; Phyllanthus niruri; Protein Isolate

Indexed keywords

ALANINE AMINOTRANSFERASE; CARBON TETRACHLORIDE; CATALASE; FREE RADICAL; LACTATE DEHYDROGENASE; PHYLLANTHUS NIRURI EXTRACT; SCAVENGER; SUPEROXIDE DISMUTASE;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ANTIOXIDANT ACTIVITY; ARTICLE; CELL SUSPENSION; CELL VIABILITY; CONTROLLED STUDY; HERBAL MEDICINE; IN VITRO STUDY; LIPID PEROXIDATION; LIVER CELL; LIVER PROTECTION; LIVER TOXICITY; MALE; MOUSE; NONHUMAN; OXIDATIVE STRESS; PRIORITY JOURNAL; PROTEIN ISOLATION; SPECTROPHOTOMETRY; STATISTICAL ANALYSIS;

PHYLLANTHUS NIRURI;

EID: 33845234243     PISSN: 15376516     EISSN: 15376524     Source Type: Journal    
DOI: 10.1080/15376510600970034     Document Type: Article

References (31)

1. Bei, W., Peng, W., Ma, Y., and Xu, A. 2004. NaoXinQing, an anti-stroke herbal medicine, reduces hydrogen peroxide-induced injury in NG108-15 cells. Neurosci. Lett. 363:262-265.
2. Blois, M. S. 1958. Antioxidant determination by use of a stable free radical. Nature 29:1199-1200.
3. Bonaventura, J., Schroeder, W. A., and Fang, S. 1972. Human erythrocyte catalase: an improved method of isolation and a revaluation of reported properties. Arch. Biochem. Biophys. 150:606-617.
4. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
5. Chauhan, C. K., Nanivadekar, S. A., and Billimoria, F. R. 1992. Effect of a herbal hepatoprotective product on drug metabolism in patients of cirrhosis and hepatic enzyme function in experimental liver damage. Ind. J. Pharmacol. 24:107-110.
6. Del Raso, N. 1992. In vitro methods for assessing chemical or drug toxicity and metabolism in primary hepatocytes. In: Watson, R. R. (Ed.), In vitro methods of toxicology, CRC Press Inc, Boca Raton, Fl, pp. 176-201.
7. Esterbauer, H., and Cheeseman, K. H. 1990. Determination of aldehydic lipid peroxidation products: malonaldehyde and 4-hydroxynonenal. Methods Enzymol. 186:407-421.
8. Guerra, M. C., Speroni, E., Broccoli, M., Cangini, M., Pasini, P., Minghett, A., et al 2000. Comparison between chinese medical herb Pueraria lobata crude extract and its main isoflavone puerarin antioxidant properties and effects on rat liver CYP-catalysed drug metabolism. Life Sci. 67:2997-3006.
9. Gyamfi, M. A., and Aniya, Y. 2002. Antioxidant properties of Thonningianin A, isolated from the African medicinal herb, Thonningia sanguinea. Biochem. Pharmacol. 63:1725-1737.
10. 4 poisoning?. Free Radic. Biol. Med. 28:838-845.
11. Lin, T. J., Su, C. C., Lan, C. K., Jiang, D. D., Tsa, J. L., and Tsai, M. S. 2003. Acute poisonings with Breynia officinalis - an outbreak of hepatotoxicity. J. Toxicol. Clin. Toxicol. 41:591-594.
12. Malhi, H., Irani, A. N., Gagandeep, S. G., and Gupta, S. 2002. Isolation of human progenitor liver epithelial cells with extensive replication capacity and differentiation into mature hepatocytes. J. Cell Sci. 115:2679-2688.
13. Myagmar, B. E., Shinno, E., Ichiba, T., and Aniya, Y. 2004. Antioxidant activity of medicinal herb Rhodococcum vitis-idaea on galactosamine-induced liver injury in rats. Phytomedicine 11:416-23.
14. Naik, A. D., and Juvekar, A. R. 2003. Effects of alkaloidal extract of Phyllanthus niruri on HIV replication. Ind. J. Med. Sci. 57:387-393.
15. Noguchi, T., Fong, K. L., Lai, E. K., Alexander, S. S., King, M. M., Olson, L., Poyer, J. L., and Mccay, P. B. 1982. Specificity of aphenobarbital-induced cytochrome P450 for metabolism of carbontetrachloride to the trichloromethyl radical. Biochem. Pharmacol. 31:615-624.
16. Packer, J. E., Slater, T. F., and Willson, R. L. 1978. Reactions of the carbon tetrachlonde-related peroxy free radical with aminoacids: pulse radiolysis evidence. Life Sci. 23:2617-2620.
17. 4-induced mitochondrial dysfunction. Life. Sci. 64:2411-2417.
18. Paoletti, F., Aldinucci, D., Mocali, A., and Caparrini, A. 1986. A sensitive spectrophotometric method for the determination of superoxide dismutase activity in tissue extracts. Anal. Biochem. 154:536-541.
19. Poyer, J. L., Mccay, P. B., Lai, E. K., Jenzen, E. G., and Davis, E. R. 1980. Confirmation of assignment of the trichloromethyl radicalspin adduct detected by spin trapping during ′3C-carbon tetrachloride metabolism in vitro and in vivo. Biochem. Biophys. Res. Commun. 94:1154-1160.
20. Recknagel, R. O., Glendek, E. A. Jr., Dolazk, J. A., and Waller, R. L. 1989. Mechanism of carbon tetrachloride toxicity. Pharmacol. Ther. 43:139-154.
21. Reitman, S., and Frankel, S. 1957. A colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transminases. Am. J. of Clini. Pathol. 28:56-63.
22. Searey, R. L. 1969. Diagnostic Biochemistry, McGrane-Hill, New York, NT.
23. Sebastian, T., and Setty, O. H. 1999. Protective effect of Phyllanthus against ethanol-induced mitochondrial dysfunction. Alcohol 17:29-34.
24. Stal, P., and Olson, J. 2000. Ubiquinone, oxidative stress, and liver carcinogenesis. In: Kagan, Ve., and Quinn, D. J. (Eds.), Coenzyme Q: Molecular Mechanisms in Health and Disease, CRC Press, Boca Raton, pp. 317-329.
25. Syamasundar, K. V., Singh, B., Thakur, R. S., Husain, A., Kiso, Y., and Hikino, H. 1985. Antihepatotoxic principles of Phyllanthus niruri herbs. J. Ethnopharmacol. 14:41-44.
26. Thyagarajan, S. P., Subramanian, S., Thirunalasundari, T., Venkateswaran, P. S., and Blumberg, B. S. 1988. Effect of Phyllanthus amarus on chronic carriers of hepatitis B virus. Lancet 2(8614):764-766.
27. Unander, D. W., Webster, G. L., and Blumberg, B. S. 1995. Usage and bioassays in Phyllanthus (Euphorbiaceae). IV. Clustering of antiviral uses and other effects. J. Ethnopharmacol. 45:1-18.
28. Venkateswaran, P. S., Millman, I., and Blumberg, B. S. 1987. Effects of an extract from Phyllanthus niruri on hepatitis B and woodchuck hepatitis viruses: in vitro and in vivo studies. Proc. Natl. Acad. Sci. U S A. 84:274-278.
29. Wei, Y. H. 1998. Oxidative stress and mitochondrial DNA mutations in human aging. Proc. Soc. Exp. Boil. Med. 217:53-63.
30. Yamamoto, Y., Yamashita, S., Fujisawa, A., Kokura, S., and Yoshikawa, T. 1998. Oxidative stress in patients with hepatitis, cirrhosis and hepatoma, evaluated by plasma antioxidants. Biochem. Biophys. Res. Commun. 247:166-170.
31. Yamamoto, Y., and Yamashita, S. 1999. Plasma ubiquinone to ubiquinol ratio in patients with hepatitis, cirrhosis, and hepatoma, and in patients treated with percutaneous transluminal coronary reperfusion. Bio Factors 9:241-245.