A novel nitric oxide cellular biosensor based on red blood cells immobilized on gold nanoparticles

Analytical Letters

Volumn 39, Issue 15, 2006, Pages 2849-2859

  Gu, Hai Ying ^a   Lu, Shuang Yun ^a   Jiang, Qi Yu ^a   Yu, Chun Mei ^a   Li, Genxi ^b   Chen, Hong Yuan ^c  

^a NANTONG UNIVERSITY   (China)

^b NANJING UNIVERSITY   (China)

^c NANJING UNIVERSITY   (China)

Author keywords

Cellular biosensors; Glassy carbon electrode; Gold colloid; Nitric oxide (NO); Red blood cells (RBCs, erythrocytes)

Indexed keywords

EID: 33845221993     PISSN: 00032719     EISSN: 1532236X     Source Type: Journal    
DOI: 10.1080/00032710600867457     Document Type: Article

References (23)

1. Bendayan, M. 1989. The enzyme-gold cytochemical approach. In Colloidal Gold: Principles, Methods, and Applications; Hayat, M.A. (ed.); Academic Press: San Diego, Calif.; Vol. 2, chapter 2, 117-147.
2. Doron, A., Katz, E., and Willner, I. 1995. Organization of Au colloids as monolayer films onto ITO glass surfaces: application of the metal colloid films as base interfaces to construct redox-active monolayers. Langmuir, 11: 1313-1317.
3. Fang, B., Wang, G.F., Yang, X.H., Zha, Q., Zhang, W., and Kan, X. 2004. Electrochemistry of hemoglobin on a gold colloid-1, 4-Benzenedimethanethiol modified electrode and electrocatalyte detection of hydrogen peroxide. Anal. Lett., 37: 2911-2924.
4. Feng, J.J., Zhao, G., Xu, J.J., and Chen, H.Y. 2005. Direct electrochemistry and electrocatalysis of heme proteins immobilized on gold nanoparticles stabilized by chitosan. Anal. Biochem., 342: 280-286.
5. Gu, H.Y., Chen, Z., Sa, R.X., Yuan, S.S., Chen, H.Y., Ding, Y., and Yu, A.M. 2004a. The immobilization of hepatocytes on 24 nanometer sized gold colloid for enhanced hepatocytes proliferation. Biomaterials, 25: 3445-3451.
6. Gu, H.Y., Xu, Y., Peng, W., Li, G., and Chen, H.Y. 2004b. A novel method for separating the anodic voltammetric peaks of dopamine and ascorbic acid. Microchim. Acta, 146: 223-227.
7. Gu, H.Y., Sa, R.X., Yuan, S.S., Chen, H.Y., and Yu, A.M. 2003. The self-assembly, characterization of hepatocytes on nano-sized gold colloid and construction of cellular biosensor. Chem. Lett., 32: 934-935.
8. Gu, H.Y., Yu, A.M., Yuan, S.S., and Chen, H.Y. 2002. Amperometric nitric oxide biosensor based on the immobilization of hemoglobin on a nano-sized gold colloid modified Au electrode. Anal. Lett., 35: 647-661.
9. 6, and C at electrochemically pretreated glassy carbon electrode. Anal. Lett., 34: 2361-2374.
10. Gu, H.Y., Yu, A.M., and Chen, H.Y. 2001b. Direct electron transfer and characterization of hemoglobin immobilized on a Au colloid-cysteamine-modified gold electrode. J. Electroanal. Chem., 561: 119-126.
11. 3-/4 on Au colloids as monolayer films on cysteamine modified gold electrode. J. Electroanal. Chem., 466: 26-30.
12. Kamin, R.A. and Wilson, G.S. 1980. Rotating ring-disk enzyme electrode for biocatalysis kinetic studies and characterization of the immobilized enzyme layer. Anal. Chem., 52: 1198-1205.
13. Laviron, E. 1979. The use of linear potential sweep voltammetry and of A. C. Voltammetry for the study of the surface electrochemical reaction of strongly adsorbed systems and of redox modified electrodes. J. Electroanal. Chem., 100: 263-270.
14. Liu, S.Q. and Ju, H.X. 2003. Nitrite reduction and detection at a carbon paste electrode containing hemoglobin and colloidal gold. Analyst, 128: 1420-1424.
15. Mena, M.L., Yanez-Sedeno, P., and Pingarron, J.M. 2005. A comparison of different strategies for the construction of amperometric enzyme biosensors using gold nanoparticle-modified electrodes. Anal. Biochem., 336: 20-27.
16. Willner, I., Katz, E., and Willner, B. 1997. Electrical contact of redox enzyme layers associated with electrodes: routes to amperometric biosensors. Electroanalysis, 9: 965-977.
17. Xiao, Y., Ju, H.X., and Chen, H.Y. 1999. Hydrogen peroxide sensor based on horseradish peroxidase-labeled Au colloids immobilized on gold electrode surface by cysteamine monolayer. Anal. Chim. Acta, 391: 73-82.
18. Xiao, Y., Ju, H.X., and Chen, H.Y. 2000. Direct electrochemistry of horseradish peroxidase immobilized on a colloid/cysteamine-modified gold electrode. Anal. Biochem., 278: 22-28.
19. Xiao, Y., Patolsky, F., Katz, E., Hainfeld, J.F., and Willner, I. 2003. Plugging into enzymes: nanowiring of redox enzymes by a gold nanoparticle. Science, 299: 1877-1881.
20. Xu, X.X., Liu, S.Q., and Ju, H.X. 2004. Disposable biosensor based on a hemoglobin colloidal gold-modified screen-printed electrode for determination of hydrogen-peroxide. IEEE Sensors J., 4: 390-394.
21. Zhang, J.D. and Oyama, M. 2005. Gold nanoparticle-attached ITO as a biocompatible matrix for myoglobin immobilization: direct electrochemistry and catalysis to hydrogen peroxide. J. Electroanal. Chem., 577: 273-279.
22. Zhang, J.D. Oyama, M. 2004. A hydrogen peroxide sensor based on the peroxidase activity of hemoglobin immobilized on gold nanoparticles-modified ITO electrode. Electrochim. Acta, 50: 85-90.
23. Zhao, J. Henkens, R.W. Stonehuerner, J.O'Daly, J.P. Crumbliss, A.L. 1992. Direct electron transfer at horseradish peroxidase-colloidal gold modified electrodes. J. Electroanal. Chem., 327: 109-119.