Cytoskeleton in trichomonads: I. Immunological and biochemical comparative study of costal proteins in the genus Tritrichomonas

European Journal of Protistology

Volumn 29, Issue 2, 1993, Pages 160-170

  Viscogliosi, Eric ^a,b   Brugerolle, Guy ^a  

^a CNRS   (France)

^b ROCKEFELLER UNIVERSITY   (United States)

Author keywords

Costa; Cytoskeleton; dimethyl sulfoxide; DMSO; EGTA; Electrophoresis; ethyleneglycol bis ( aminoethyl ether) N, N, N , N tetraacetic acid; MEM; minimum essential medium; Monoclonal antibodies; N p tosyl L arginine methyl ester; N p tosyl L lysine chloromethyl ketone; SDS PAGE; sodium dodecyl sulphate Polyacrylamide gel electrophoresis; TAME; TLCK; Trichomonads

Indexed keywords

EID: 33845206749     PISSN: 09324739     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0932-4739(11)80269-5     Document Type: Article

References (49)

1. Characterization of a 33-kilodalton structural protein of Giardia lamblia and localization to the ventral disk
2. Structure, protein composition and birefringence of the costa: a motile flagellar root fibre in the flagellate Trichomonas
3. Cytologie ultrastructurale, systématique et évolution des Trichomonadida
4. Flagellar and cytoskeletal systems in amitochondrial flagellates: Archamoebae, Metamonada and Parabasala
5. Advances in specimen preparation for electron microscopy. I. Novel low temperature embedding resins and a reformulated vestopal
6. Characterization of proteins from the cytoskeleton of Giardia lamblia
7. Assembly of 2.5 nm filaments from giardin, a protein associated with cytoskeletal microtubules in Giardia
8. High resolution light and electron microscopy localization of tubulin with IGS (immunogold staining)
9. Structure and protein composition of the striated flagellar rootlets of some protists
10. The establishment of various tricho-monads of animals and man in axenic cultures
11. Preparation of monoclonal antibodies: strategies and procedures
12. Isolation and characterization of costa in Tritrichomonas augusta
13. SDS Polyacrylamide gel electrophoresis of trichomonad costa proteins
14. The cytoskeleton in protists: nature, structure and functions
15. Segmental α-helical coiled-coil structure of the protein giardin from the Giardia cytoskeleton
16. Structure
17. Fine structure of Trichomonas batrachorum (Perti)
18. Purification and characterization of a basal body-associated Ca2+-binding protein
19. 2+-binding protein: homology in its protein sequence with calmodulin and the yeast CDC31 gene product
20. Identification of proteins of the striated rootlets of Tetrahymena by immunofluorescence microscopy and immunoblotting with an anti-rootlet serum
21. Basic structure and function of the trichomonad cell
22. Molecular weight differences in polypeptides from type A and B trichomonad costae
23. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
24. Isolation, ultrastructure and protein composition of the flagellar rootlet of Naegleria gruberi
25. Striated microtu-bule-associated fibers: identification of assemblin, a novel 34-KD protein that forms paracrystals of 2 nm filaments in vitro
26. Immunocytochemical study on the formation of striated rootlets during ciliogenesis in quail oviduct
27. The flagellar apparatus of Dunaliella. Isolation of basal body-flagellar root complex
28. An update on fibrous flagellar roots in green algae
29. A polyclonal antibody (anti-centrin) distinguishes between two types of fibrous flagellar roots in green algae
30. Monoclonal antibodies
31. Ultrastructural localization of giardins to the edges of disk microribbons of Giardia lamblia and the nucleotide and deduced protein sequence of alpha giardin
32. Basel bodies and root structures
33. All classes of intermediate filaments share a common antigenic determinant defined by a monoclonal antibody
34. The use of lead citrate and high pH as an electron opaque stain in electron microscopy
35. Partial purification and phosphotungstate solubilization of basal bodies and kinetodesmal fibres from Tetrahymena pyriformis
36. Calcium-induced contraction of the rhizoplast of a quadriflagellate green alga
37. Striated flagellar roots: isolation and partial characterization of a calcium modulated contractile organelle
38. Calcium modulated contractile proteins associated with the eukaryotic centrosome
39. Molecular polymorphism of ciliary proteins from different species of the ciliate Tetrahymena
40. Cortical morphogenesis in Paramecium: a transcellular wave of protein phosphorylation involved in ciliary rootlet disassembly
41. The molecular biology of intermediate filaments
42. The basal apparatus. Mass isolation from the molluscan ciliated gill epithelium and a preliminary characterization of striated rootlets
43. Electrophor-etic transfer of proteins from acrylamide gels to nitrocellulose sheets: procedure and some applications
44. Biochemical and immunological characterization of the microfibrillar ecto-endoplasmic boundary in the ciliate Isotricha prostoma
45. Molecular phylogeny of trichomonads based on partial sequences of large subunit rRNA and comparison with cladistic analysis of morphological data
46. An intermediate filament associated protein PSO recognized by monoclonal antibodies
47. Protein similarities in the genus Tetrabymena and a description of Tetrabymena leucopbrys n. sp.
48. Introduction to cilia and flagella
49. Centrin- and actinin-like immunoreac-tivity in the ciliary rootlets of insect sensilla