Rapid sequencing of the non-coding regions of influenza A virus

Journal of Virological Methods

Volumn 139, Issue 1, 2007, Pages 85-89

  de Wit, Emmie ^a   Bestebroer, Theo M ^a   Spronken, Monique I J ^a   Rimmelzwaan, Guus F ^a   Osterhaus, Albert D M E ^a   Fouchier, Ron A M ^a  

^a ERASMUS MEDICAL CENTER   (Netherlands)

Author keywords

Influenza A virus; Ligation; NCR; PCR; Sequencing

Indexed keywords

UNTRANSLATED RNA; VIRUS RNA;

ARTICLE; BIOTECHNOLOGICAL PROCEDURES; GENE SEQUENCE; INFLUENZA VIRUS A; MOLECULAR CLONING; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REVERSE TRANSCRIPTION; VIRUS GENE;

INFLUENZA A VIRUS, H3N2 SUBTYPE; INFLUENZA A VIRUS, H5N1 SUBTYPE; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SEQUENCE ANALYSIS, RNA; UNTRANSLATED REGIONS;

INFLUENZA A VIRUS;

EID: 33845193024     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2006.09.015     Document Type: Article

References (15)

1. Brownlee G.G., and Sharps J.L. The RNA polymerase of influenza a virus is stabilized by interaction with its viral RNA promoter. J. Virol. 76 (2002) 7103-7113
2. Chen Z. Simple modifications to increase specificity of the 5′ RACE procedure. Trends Genet. 12 (1996) 87-88
3. de Wit E., Spronken M.I., Bestebroer T.M., Rimmelzwaan G.F., Osterhaus A.D., and Fouchier R.A. Efficient generation and growth of influenza virus A/PR/8/34 from eight cDNA fragments. Virus Res. 103 (2004) 155-161
4. Desselberger U., Racaniello V.R., Zazra J.J., and Palese P. The 3′ and 5′-terminal sequences of influenza A, B and C virus RNA segments are highly conserved and show partial inverted complementarity. Gene 8 (1980) 315-328
5. Frohman M.A., Dush M.K., and Martin G.R. Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer. Proc. Natl. Acad. Sci. U.S.A. 85 (1988) 8998-9002
6. Ghedin E., Sengamalay N.A., Shumway M., Zaborsky J., Feldblyum T., Subbu V., Spiro D.J., Sitz J., Koo H., Bolotov P., Dernovoy D., Tatusova T., Bao Y., St George K., Taylor J., Lipman D.J., Fraser C.M., Taubenberger J.K., and Salzberg S.L. Large-scale sequencing of human influenza reveals the dynamic nature of viral genome evolution. Nature 437 (2005) 1162-1166
7. Hsieh S.Y., Yang P.Y., Chen H.C., and Liaw Y.F. Cloning and characterization of the extreme 5′-terminal sequences of the RNA genomes of GB virus C/hepatitis G virus. Proc. Natl. Acad. Sci. U.S.A. 94 (1997) 3206-3210
8. Lee K.H., and Seong B.L. The position 4 nucleotide at the 3′ end of the influenza virus neuraminidase vRNA is involved in temporal regulation of transcription and replication of neuraminidase RNAs and affects the repertoire of influenza virus surface antigens. J. Gen. Virol. 79 (1998) 1923-1934
9. Lee M.K., Bae S.H., Park C.J., Cheong H.K., Cheong C., and Choi B.S. A single-nucleotide natural variation (U4 to C4) in an influenza A virus promoter exhibits a large structural change: implications for differential viral RNA synthesis by RNA-dependent RNA polymerase. Nucleic Acids Res. 31 (2003) 1216-1223
10. Lee M.T., Klumpp K., Digard P., and Tiley L. Activation of influenza virus RNA polymerase by the 5′ and 3′ terminal duplex of genomic RNA. Nucleic Acids Res. 31 (2003) 1624-1632
11. Liu X., and Gorovsky M.A. Mapping the 5′ and 3′ ends of Tetrahymena thermophila mRNAs using RNA ligase mediated amplification of cDNA ends (RLM-RACE). Nucleic Acids Res. 21 (1993) 4954-4960
12. Luytjes W., Krystal M., Enami M., Pavin J.D., and Palese P. Amplification, expression, and packaging of foreign gene by influenza virus. Cell 59 (1989) 1107-1113
13. Poon L.L., Pritlove D.C., Fodor E., and Brownlee G.G. Direct evidence that the poly (A) tail of influenza A virus mRNA is synthesized by reiterative copying of a U track in the virion RNA template. J. Virol. 73 (1999) 3473-3476
14. Szymkowiak C., Kwan W.S., Su Q., Toner T.J., Shaw A.R., and Youil R. Rapid method for the characterization of 3′ and 5′ UTRs of influenza viruses. J. Virol. Methods 107 (2003) 15-20
15. Tiley L.S., Hagen M., Matthews J.T., and Krystal M. Sequence-specific binding of the influenza virus RNA polymerase to sequences located at the 5′ ends of the viral RNAs. J. Virol. 68 (1994) 5108-5116