Activation of ribosomal RNA genes in porcine embryos produced in vitro or by somatic cell nuclear transfer

Molecular Reproduction and Development

Volumn 74, Issue 1, 2007, Pages 35-41

  Bjerregaard, Bolette ^a   Pedersen, Hanne G ^a   Jakobsen, Anne S ^a   Rickords, Lee F ^b   Lai, Liangxue ^c   Cheong, Hee Tae ^c   Samuel, Melissa ^c   Prather, Randall S ^c   Strejcek, Frantisek ^d   Rasmussen, Zaida R ^a   Laurincik, Jozef ^d,e   Niemann, Heiner ^f   Maddox Hyttel, Poul ^a   Thomsen, Preben D ^a  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

^b UTAH STATE UNIVERSITY   (United States)

^c UNIVERSITY OF MISSOURI   (United States)

^d Department of Ethics and Aesthetics   (Slovakia)

^e RESEARCH INSTITUTE OF ANIMAL PRODUCTION   (Slovakia)

^f INSTITUT FÜR TIERZUCHT   (Germany)

Author keywords

Embryonic genome activation; FISH; Nucleolus

Indexed keywords

NUCLEAR PROTEIN; RIBOSOME DNA; RIBOSOME RNA;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; BLASTOCYST; CELL CYCLE; CELL NUCLEUS TRANSPLANTATION; DNA PROBE; EMBRYO; EMBRYO DEVELOPMENT; FEMALE; FETUS; FLUORESCENCE IN SITU HYBRIDIZATION; GENE ACTIVATION; IN VITRO STUDY; IN VIVO STUDY; NONHUMAN; NUCLEOLUS; PRIORITY JOURNAL; PROTEIN ANALYSIS; RNA GENE; RNA SYNTHESIS; RNA TRANSCRIPTION; SILVER STAINING; SOMATIC CELL GENETICS; SWINE; TRANSCRIPTION INITIATION; TRANSCRIPTION REGULATION;

ANIMALS; EMBRYO; EMBRYONIC DEVELOPMENT; FERTILIZATION IN VITRO; GENES, RRNA; NUCLEAR TRANSFER TECHNIQUES; RNA, RIBOSOMAL; SUS SCROFA; TRANS-ACTIVATION (GENETICS); TRANSCRIPTION, GENETIC;

SUIDAE;

EID: 33751577332     PISSN: 1040452X     EISSN: 10982795     Source Type: Journal    
DOI: 10.1002/mrd.20594     Document Type: Article

References (15)

1. Bjerregaard B, Wrenzycki C, Strejcek F, Laurincik J, Holm P, Ochs RL, Rosenkranz C, Callesen H, Rath D, Niemann H, Maddox-Hyttel P. 2004. Expression of nucleolar-related proteins in porcine preimplantation embryos produced in vivo and in vitro. Biol Reprod 70:867-876.
2. Cabot R, Khlholzer B, Chan AW, Lai L, Park KW, Chong KY, Schatten G, Murphy CN, Abeydeera LR, Day BN, Prather RS. 2001. Production of transgenic piglets derived from in vitro matured oocytes infected with a replication deficient retroviral vector. Anim Biotech 12:205-214.
3. Hyttel P, Laurincik J, Rosenkranz C, Rath D, Niemann H, Ochs RL, Schellander K. 2000. Nucleolar proteins and ultrastructure in preimplantation porcine embryos developed in vivo. Biol Reprod 63:1848-1856.
4. Jakobsen A. 2005. Cell biological studies of in vitro produced bovine embryos. PhD Thesis. Frederiksberg: Samfundslitteratur Grafik, Denmark, p 1-75. ISBN 87-7611-104-0.
5. Kopecny V. 1989. High-resolution autoradiographic studies of comparative nucleologenesis and genome reactivation during early embryogenesis in pig, man and cattle. Reprod Nutr Dev 29:589-600.
6. Lai LX, Kolber-Simonds D, Park KW, Cheong HT, Greenstein JL, Im GS, Samuel M, Bonk A, Rieke A, Day BN, Murphy CN, Carter DB, Hawley RJ, Prather RS. 2002. Production of alpha-1,3-galactosyltransferase knockout pigs by nuclear transfer cloning. Science 295:1089-1092.
7. Laurincik J, Bjerregaard B, Strejcek F, Rath D, Niemann H, Rosenkranz C, Ochs RL, Maddox-Hyttel P. 2004. Nucleolar ultrastructure and protein allocation in in vitro produced porcine embryos. Mol Reprod Dev 68:327-334.
8. Lindner LE. 1993. Improvements in the silver-staining technique for nucleolar organizer regions (AgNOR). J Histochem Cytochem 41:439-445.
9. Niemann H, Rath D. 2001. Progress in reproductive biotechnology in swine. Theriogenology 56:1291-1304.
10. Polejaeva IA, Chen SH, Vaught TD, Page RL, Mullins J, Ball S, Dai Y, Boone J, Walker S, Ayares DL, Colman A, Campbell KH. 2000. Cloned pigs produced by nuclear transfer from adult somatic cells. Nature 407:86-90.
11. Rath D, Long CR, Dobrinsky JR, Welch GR, Schreier LL, Johnson LA. 1999. In vitro production of sexed embryos for gender preselection: High-speed sorting of X-chromosome-bearing sperm to produce pigs after embryo transfer. J Anim Sci 77:3346-3352.
12. Sirri V, Roussel P, Hernandez-Verdun D. 2000. The AgNOR proteins: Qualitative and quantitative changes during the cell cycle Micron 31:121-126.
13. Viuff D, Greve T, Avery B, Hyttel P, Brockhoff P, Thomsen PD. 2000. Chromosome aberrations in in vitro produced bovine embryos at days 2-5 post-insemination. Biol Reprod 63:1143-1148.
14. Viuff D, Greve T, Holm P, Callesen H, Hyttel P, Thomsen PD. 2002. Activation of the ribosomal RNA genes late in the third cell cycle of porcine embryos. Biol Reprod 66:629-634.
15. Yerle M, LahbibMansais Y, Pinton P, Robic A, Goureau A, Milan D, Gellin J. 1997. The cytogenetic map of the domestic pig (Sus scrofa domestica). Mamm Genome 8:592-607.