Application of the DNA-specific dye EvaGreen for the routine quantification of DNA in microplates

Analytical Biochemistry

Volumn 359, Issue 2, 2006, Pages 265-267

  Ihrig, Jessica ^a   Lill, Roland ^a   Mühlenhoff, Ulrich ^a  

^a PHILIPPS UNIVERSITY MARBURG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY;

MICROPLATES; QUANTIFICATION OF DNAS;

CHEMISTRY;

COMPLEMENTARY DNA; DNA; EVAGREEN; FLUORESCENT DYE; RNA;

ARTICLE; CALIBRATION; COMPLEX FORMATION; DNA DETERMINATION; DNA ISOLATION; EXCITATION; FLAME PHOTOMETRY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SENSITIVITY ANALYSIS; SIGNAL DETECTION; SPECTROMETER;

EID: 33751393172     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2006.07.043     Document Type: Article

References (6)

1. Sambrook J., and Russel D.W. Molecular Cloning: A Laboratory Manual. third ed. (2001), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
2. Wilson K., and Walker J. Principles and Techniques of Practical Biochemistry. fifth ed. (1999), Cambridge University Press, Cambridge, UK
3. Singer V.L., Jones L.J., Yue S.T., and Haugland R.P. Characterization of PicoGreen reagent and development of a fluorescence-based solution assay for double-stranded DNA quantitation. Anal. Biochem. 249 (1997) 228-238
4. Batchelor R., Hagen D., Johnson I., and Beechem J. A fluorescent high-throughput assay for double-stranded DNA: The RediPlate PicoGreen assay. Comb. Chem. High Throughput Scr. 6 (2003) 287-291
5. Amberg D.C., Burke D., and Strathern J.N. Methods in Yeast Genetics (2005), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
6. Schofield G.G. PicoGmeter, a custom-made fluorometer for the quantification of dsDNA by PicoGreen fluorescence. BioTechniques 37 (2004) 778-782