Localized and non-contact mechanical stimulation of dorsal root ganglion sensory neurons using scanning ion conductance microscopy

Journal of Neuroscience Methods

Volumn 159, Issue 1, 2007, Pages 26-34

  Sánchez, Daniel ^a   Anand, Uma ^b   Gorelik, Julia ^c   Benham, Christopher D ^d   Bountra, Chas ^d   Lab, Max ^a   Klenerman, David ^e   Birch, Rolfe ^b   Anand, Praveen ^b   Korchev, Yuri ^a  

^a LONDON CENTRE FOR NANOTECHNOLOGY   (United Kingdom)

^b IMPERIAL COLLEGE LONDON   (United Kingdom)

^c NATIONAL HEART AND LUNG INSTITUTE   (United Kingdom)

^d GLAXOSMITHKLINE   (United Kingdom)

^e UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

Human DRG; Mechanosensitive ion channels; Nanopipette; Neurons; Pain; Rat DRG; SPM

Indexed keywords

ADULT; ANALYTIC METHOD; ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; CALCIUM CELL LEVEL; CELL DAMAGE; CELL MEMBRANE; CONCENTRATION RESPONSE; HUMAN; HUMAN CELL; ION CONDUCTANCE; MECHANICAL STIMULATION; MICROSCOPY; MOLECULAR PROBE; NEURITE; NONHUMAN; PATCH CLAMP; PRIORITY JOURNAL; RAT; SENSORY NERVE CELL; SPINAL GANGLION; VALIDATION PROCESS; WHOLE CELL;

ADULT; ANIMALS; CALCIUM SIGNALING; CELL MEMBRANE; CELLS, CULTURED; DENDRITES; ELECTROPHYSIOLOGY; GANGLIA, SPINAL; HUMANS; MALE; MICROSCOPY, ELECTRON, SCANNING; MICROSCOPY, FLUORESCENCE; NEURITES; NEURONS, AFFERENT; PHYSICAL STIMULATION; PRESYNAPTIC TERMINALS; RATS; RATS, WISTAR;

EID: 33751341784     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2006.06.018     Document Type: Article

References (23)

1. Anand U., Otto W.R., Casula M.A., Day N.C., Davis J.B., Countra C., et al. The effect of neurotrophic factors on morphology, TRPV1 expression and capsaicin responses of cultured human DRG sensory neurons. Neurosci Lett 399 (2006) 51-56
2. Bevan S., and Yeats J. Protons activate a cation conductance in a sub-population of rat dorsal root ganglion neurones. J Physiol 433 (1991) 145-161
3. Cho H., Shin J., Shin C.Y., Lee S.Y., and Oh U. Mechanosensitive ion channels in cultured sensory neurons of neonatal rats. J Neurosci 22 (2002) 1238-1247
4. Drew L.J., Rohrer D.K., Price M.P., Blaver K.E., Cockayne D.A., Cesare P., et al. Acid-sensing ion channels ASIC2 and ASIC3 do not contribute to mechanically activated currents in mammalian sensory neurones. J Physiol 556 (2004) 691-710
5. Drew L.J., Wood J.N., and Cesare P. Distinct mechanosensitive properties of capsaicin-sensitive and -insensitive sensory neurons. J Neurosci 22 (2002) RC228
6. Farris H.E., LeBlanc C.L., Goswami J., and Ricci A.J. Probing the pore of the auditory hair cell mechanotransducer channel in turtle. J Physiol 558 (2004) 769-792
7. Ghazi A., Berrier C., Ajouz B., and Besnard M. Mechanosensitive ion channels and their mode of activation. Biochimie 80 (1998) 357-362
8. Gschossmann J.M., Chaban V.V., McRoberts J.A., Raybould H.E., Young S.H., Ennes H.S., et al. Mechanical activation of dorsal root ganglion cells in vitro: comparison with capsaicin and modulation by kappa-opioids. Brain Res 856 (2000) 101-110
9. Heidemann S.R. A new twist on integrins and the cytoskeleton. Science 260 (1993) 1080-1081
10. Kong C.R., Bursac N., and Tung L. Mechanoelectrical excitation by fluid jets in monolayers of cultured cardiac myocytes. J Appl Physiol 98 (2005) 2328-2336
11. Korchev Y.E., Bashford C.L., Milovanovic M., Vodyanoy I., and Lab M.J. Scanning ion conductance microscopy of living cells. Biophys J 73 (1997) 653-658
12. Korchev Y.E., Milovanovic M., Bashford C.L., Bennett D.C., Sviderskaya E.V., Vodyanoy I., et al. Specialized scanning ion-conductance microscope for imaging of living cells. J Microsc 188 Pt 1 (1997) 17-23
13. Kung C. A possible unifying principle for mechanosensation. Nature 436 (2005) 647-654
14. McCarter G.C., Reichling D.B., and Levine J.D. Mechanical transduction by rat dorsal root ganglion neurons in vitro. Neurosci Lett 273 (1999) 179-182
15. 2+ release in sensory neurons: low gain amplification confers intrinsic stability. J Biol Chem 280 (2005) 15898-15902
16. Ricci A.J., and Fettiplace R. The effects of calcium buffering and cyclic AMP on mechano-electrical transduction in turtle auditory hair cells. J Physiol 501 (1997) 111-124
17. Su X., Wachtel R.E., and Gebhart G.F. Mechanosensitive potassium channels in rat colon sensory neurons. J Neurophysiol 84 (2000) 836-843
18. Sukharev S., and Corey D.P. Mechanosensitive channels: multiplicity of families and gating paradigms. Sci STKE 219 (2004) re4
19. Takeda M., Nishikawa T., Sato S., Aiyama S., and Matsumoto S. Effects of gadolinium and tetrodotoxin on the response of slowly adapting type I mechanoreceptors to mechanical stimulation in frog dorsal skin. J Peripher Nerv Syst 8 (2003) 271-281
20. Wang N., Butler J.P., and Ingber D.E. Mechanotransduction across the cell surface and through the cytoskeleton. Science 260 (1993) 1124-1127
21. Willis W.D., and Coggeshall R.E. Sensory mechanisms of the spinal cord. 3rd ed. (2004), Plenum Press, New York
22. Winkelmann R.K. Sensory receptors of the skin (1986), Plenum Press, New York p. 19-81
23. Winkelmann R.K. Cutaneous sensory nerves. Semin Dermatol 7 (1988) 236-268