Dynamic unfolding of a regulatory subunit of cAMP-dependent protein kinase by capillary electrophoresis: Impact of cAMP dissociation on protein stability

Electrophoresis

Volumn 27, Issue 21, 2006, Pages 4196-4204

  Gavina, Jennilee M A ^a   Das, Rahul ^b   Britz McKibbin, Phillip ^a  

^a MCMASTER UNIVERSITY   (Canada)

^b MCMASTER UNIVERSITY   (Canada)

Author keywords

Capillary electrophoresis; Protein unfolding; Receptor ligan interactions; Recombinant protein; Thermodynamics; Type IA cAMP dependent protein kinase; Urea denaturation

Indexed keywords

BINDING ENERGY; CAPILLARY ELECTROPHORESIS; COMPLEXATION; DISSOCIATION; ENZYMES; FREE ENERGY; LIGANDS; METABOLISM; RECOMBINANT PROTEINS;

ADENOSINE MONOPHOSPHATE; CYCLIC ADENOSINE; PROTEIN KINASE; PROTEIN STABILITY; PROTEIN UNFOLDING; RECEPTOR-LIGAN INTERACTION; REGULATORY SUBUNITS; TYPE IA CYCLIC ADENOSINE MONOPHOSPHATE-DEPENDENT PROTEIN KINASE; UNFOLDINGS; UREA DENATURATION;

UREA;

BUFFER; CYCLIC AMP; CYCLIC AMP DEPENDENT PROTEIN KINASE; UREA;

ARTICLE; CAPILLARY ELECTROPHORESIS; COMPLEX FORMATION; CONTROLLED STUDY; DISSOCIATION CONSTANT; ELECTROPHORETIC MOBILITY; ENZYME DENATURATION; ENZYME STABILITY; ENZYME SUBUNIT; THERMODYNAMICS; ULTRAVIOLET RADIATION;

EID: 33751306048     PISSN: 01730835     EISSN: 15222683     Source Type: Journal    
DOI: 10.1002/elps.200600300     Document Type: Article

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