Criteria to distinguish between natural situations and illegal use of boldenone, boldenone esters and boldione in cattle. 1. Metabolite profiles of boldenone, boldenone esters and boldione in cattle urine

Steroids

Volumn 71, Issue 13-14, 2006, Pages 1078-1087

  Le Bizec, Bruno ^a   Courant, Frédérique ^a   Gaudin, Isabelle ^a   Bichon, Emanuelle ^a   Destrez, Blandine ^a   Schilt, Robert ^b   Draisci, Rosa ^c   Monteau, Fabrice ^a   André, François ^a  

^a Waters Corporation   (France)

^b TNO   (Netherlands)

^c ISTITUTO SUPERIORE DI SANITÀ   (Italy)

Author keywords

Anabolic; Boldenone; Cattle; Endogenous; Metabolite; Urine

Indexed keywords

17 BETA BOLDENONE; BIOLOGICAL MARKER; BOLDENONE; BOLDIONE; DRUG METABOLITE; UNCLASSIFIED DRUG;

ANALYTIC METHOD; ANIMAL EXPERIMENT; ARTICLE; CATTLE; COMPARATIVE STUDY; CONTROLLED STUDY; DRUG ABUSE; DRUG METABOLISM; DRUG URINE LEVEL; METABOLITE; NONHUMAN;

ANDROSTADIENES; ANIMALS; CATTLE; ESTERS; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; MALE; TESTOSTERONE;

ANIMALIA; BOS TAURUS;

EID: 33751254328     PISSN: 0039128X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.steroids.2006.09.009     Document Type: Article

References (21)

1. Poelamns S, Schilt R, Stephany RW, Le Bizec B, Draisci R, Sterk S, et al. EU expert report on boldenone: state of the art, May 13, 2003.
2. De Brabander H.F., Poelamns S., Schilt R., Stephany R.W., Le Bizec B., Draisci R., et al. Presence and metabolism of the anabolic steroid boldenone in various animal species: a review. Food Addit Contam 21 (2004) 515-525
3. Marchand P., Lebizec B., Gade C., Monteau F., and André F. Ultra trace detection of a wide range of anabolic steroids in meat by gas chromatography coupled to mass spectrometry. J Chromatogr A 867 (2000) 219-233
4. Ferchaud V., Lebizec B., Montrade M., Maume D., Monteau F., and André F. Gas chromatographic-mass spectrometric identification of main metabolites of stanozolol in cattle after oral and subcutaneous administration. J Chromatogr B 695 (1997) 269-277
5. Ferchaud V., Le Bizec B., Monteau F., Montrade M.P., and André F. Determination of testosterone exogenous character in bovine urine by gas chromatography/combustion/isotope ratio mass spectrometry. Analyst 12 (1998) 2617-2620
6. Ferchaud V., Lebizec B., Monteau F., and André F. Characterization of exogenous testosterone in livestock by gas chromatography/combustion/isotope ratio mass spectrometry: influence of feeding and age. Rapid Commun Mass Spectrom 14 (2000) 652-656
7. Schänzer W., and Donike M. Metabolism of boldenone in man: gas chromatographic/mass spectrometric identification of urinary excreted metabolites and determination of excretion rate. Biol Mass Spectrom 21 (1992) 3-16
8. Galletti F., and Gardi R. Metabolism of 1-dehydroandrostanes in man. I. Metabolism of 17-betahydroxyandrosta-1,4-dien-3-one, 17-beta-cyclopent-1-enyloxyandrosta-1,4-dien-3-one (quinbolone) and androsta-1,4-dien-3-one. Steroids 18 (1971) 39-50
9. Kim Y., Myungyoon J., and Lee W. Characterization of boldione and its metabolites in human urine by liquid chromatography/electrospray ionization mass spectrometry and gas chromatography/mass spectrometry. Rapid Commun Mass Spectrom 20 (2006) 9-20
10. Dumasia M.C., and Houghton E. Biotransformation of 1-dehydrotestosterone in the equine male castrate: identification of the neutral unconjugated and glucuronicacid conjugated metabolites in horse urine. Biomed Environ Mass Spectrom 17 (1988) 383-392
11. Dumasia M.C., Houghton E., Bradley C., and Williams D.H. Studies related to the metabolism of anabolic steroids in the horse-the metabolism of 1-dehydrotestosterone and the use of fast atom bombardment mass spectrometry in the identification of steroid conjugates. Biomed Mass Spectrom 10 (1983) 434-440
12. Van Puymbroek M., Leyssens L., Vanderzande D., Gelan J., and Raus J. Metabolites in feces can be important markers for the abuse of anabolic steroids in cattle. Analyst 123 (1998) 2449-2452
13. Puymbroeck M.V., Kuilman M., Maas R., Witkamp R., Leyssens L., Vanderzande D., et al. Identification of some important metabolites of boldenone in urine and feces of cattle by gas chromatography-mass spectrometry. Analyst 123 (1998) 2681-2686
14. Van Puymbroeck M. Identification of selective metabolites to reveal the abuse of some synthetic anabolic steroids in cattle. Limburgs Universitair Centrum, Diepenbeek, Belgium, 2000 [Thesis].
15. Schänzer W., and Donike M. Metabolism of anabolic steroids in man: synthesis and use of reference substances for identification of anabolic steroid metabolites. Anal Chim Acta 275 (1993) 23-48
16. Poelmans S., De Wasch K., Noppe H., Van Hoof N., Van Cruchten S., Le Bizec B., et al. Endogenous occurrence of some anabolic steroids in swine matrices. Food Addit Contam 22 (2005) 808-815
17. Ho E.N.M., Yiu K.C.H., Tang F.P.W., Dehennin L., Plou P., Bonnaire Y., et al. Detection of endogenous boldenone in the entire male horses. J Chromatogr B 808 (2004) 287-294
18. Arts C., Schilt R., Schreurs M., and Van Ginkel L. Boldenone is a naturally occurring (anabolic) steroid in cattle. In: Haagsma N., and Ruiter A. (Eds). Proceedings of the EuroResidue III Conference (1996), University of Utrecht, Utrecht, The Netherlands 212-217
19. Egorova O.V., Gulevskaya S.A., Puntus I.F., Filonov A.E., and Donova M.V. Production of androstenedione using mutants of Mycobacterium species. J Chem Technol Biot 77 (2002) 141-147
20. Barthakur S., Roy M.K., Bera S.K., and Ghosh A.C. Steroid transformation by mutants of Mycobacterium species with altered response to antibiotics. J Basic Microb 36 (1996) 383-387
21. Song Y.S., Jim C., and Park E.M. Identification of metabolites of phytosterols in rat feces using GC/MS. Arch Pharm Res 23 (2000) 599-604