Cloning, expression and functional activity of deoxyhypusine synthase from Plasmodium vivax

BMC Microbiology

Volumn 6, Issue , 2006, Pages

  Njuguna, James T ^a,b   Nassar, Marwa ^c   Hoerauf, Achim ^a   Kaiser, Annette E ^a,d  

^a UNIVERSITY OF BONN   (Germany)

^b INTERNATIONAL LIVESTOCK RESEARCH INSTITUTE   (Kenya)

^c THEODOR BILHARZ RESEARCH INSTITUTE   (Egypt)

^d GERMAN UNIVERSITY IN CAIRO   (Egypt)

Author keywords

[No Author keywords available]

Indexed keywords

DEOXYHYPUSINE SYNTHASE; HEPTANE DERIVATIVE; POLYCLONAL ANTIBODY;

AFFINITY CHROMATOGRAPHY; AMINO TERMINAL SEQUENCE; ARTICLE; CONTROLLED STUDY; CROSS REACTION; ENZYME ACTIVITY; GENE SEQUENCE; HUMAN; MALARIA; MOLECULAR CLOCK; NONHUMAN; NUCLEOTIDE SEQUENCE; PATHOGENICITY; PHYLOGENY; PLASMODIUM VIVAX; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; PROTEIN FUNCTION; WESTERN BLOTTING;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES, MONOCLONAL; BLOTTING, WESTERN; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; CROSS REACTIONS; GENE EXPRESSION REGULATION, ENZYMOLOGIC; HUMANS; MOLECULAR SEQUENCE DATA; OXIDOREDUCTASES ACTING ON CH-NH GROUP DONORS; PEPTIDE INITIATION FACTORS; PLASMODIUM VIVAX; PROTOZOAN PROTEINS; RECOMBINANT PROTEINS; RNA-BINDING PROTEINS; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID;

PLASMODIUM FALCIPARUM; PLASMODIUM VIVAX; PLASMODIUM YOELII; RODENTIA;

EID: 33751250507     PISSN: 14712180     EISSN: 14712180     Source Type: Journal    
DOI: 10.1186/1471-2180-6-91     Document Type: Article

References (16)

1. Carlton J, Dame J: The Plasmodium vivax and P. berghei gene sequence tag projects. Parasitol Today 2000, 10:409.
2. Cui L, Fan Q, Hu Y, Karamycheva SA, Quackenbush J, Khuntirat B, Sattabongkot J, Carlton JM: Gene discovery in Plasmodium vivax through sequencing of ESTs from mixed blood stages. Mol Biochem Parasitol 2005, 144:1-9.
3. Park MH: The Post-Translational Synthesis of a Polyamine-Derived Amino Acid, Hypusine, in the Eukaryotic Translation Initiation Factor 5A (eIF5A). J Biol Chem (Tokyo) 2006, 139:161-69.
4. Kaiser A, Gottwald A, Maier W, Seitz HM: Targeting enzymes involved in spermidine metabolism of parasitic protozoa -a possible, new strategy for antiparasitic treatment. Parasitol Res 2003, 91:508-16.
5. Studier FW, Moffat BA: Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J Mol Biol 1986, 189:113-30.
6. Jambou R, Legrand E, Niang M, Khim N, Lim P, Volney B, Ekala MT, Bauchier C, Esterre P, Fandeur T, Mercereau-Puijalon O: Resistance of Plasmodium falciparum field isolates to in-vitro artemether and point mutations of the SERCA-type PfATPase6. Lancet 2005, 366:1960-63.
7. Golenda CF, Li J, Rosenberg R: Continuous in vitro propagation of the malaria parasite Plasmodium vivax. Proc Natl Acad Sci 1997, 94:6786-91.
8. Kaiser A, Gottwald A, Wiersch C, Maier W, Seitz HM: Effect of drugs inhibiting spermidine biosynthesis and metabolism on the in vitro development of P. falciparum. Parasitol Res 2001, 87:963-72.
9. Kaiser A, Gottwald C, Wiersch C, Maier W, Seitz HM: Spermidine metabolism in parasitic protozoa-a comparison to the situation in prokaryotes, viruses, plants and fungi. Folia Parasitol 2003, 50:3-18.
10. Carlton J, Silva J, Hall N: The genome of model malaria parasites, and comparative genomics. Curr Issues Mol Biol 2005, 7:23-37.
11. Yan YP, Tao Y, Chen KY: Molecular cloning and functional expression of human deoxyhypusine synthase cDNA based on expressed sequence tag information. Biochem J 1996, 315:429-434.
12. Joe YA, Wolff EC, Lee YB, Park MH: Enzyme-substrate intermediate at a specific lysine residue is required for deoxyhypusine synthesis. The role of Lys329 in human deoxyhypusine synthase. J Biol Chem (Tokyo) 1997, 272:32679-32685.
13. Umland TC, Wolff TC, Park MH, Davies DR: A new crystal structure of deoxyhypusine synthase reveals the configuration of the active enzyme and of an enzyme NAD inhibitor ternary complex. J Biol Chem 2004, 279:28697-28705.
14. Molitor IM, Knobel S, Dang C, Spielmann T, Allera A, König GM: Translation initiation factor eIF-5A from Plasmodium falciparum. Mol Biochem Parasitol 2004, 137:65-74.
15. Liao DI, Wolff EC, Park MH, Davies DR: Crystal structure of the NAD complex of human deoxyhypusine synthase: an enzyme with a ball-and-chain mechanism for blocking the active site. Struct 1998, 6:23-32.
16. Sasaki K, Abid R, Miyazaki M: Deoxyhypusine synthase gene is essential for cell viability in the yeast Saccharomyces cerevisiae. FEBS Letters 1996, 384:151-154.