Subchronic oral toxicity of a standardized white kidney bean (Phaseolus vulgaris) extract in rats

Food and Chemical Toxicology

Volumn 45, Issue 1, 2007, Pages 32-40

  Chokshi, Dilip ^a  

^a Pharmachem Laboratories Inc   (United States)

Author keywords

Amylase inhibitor; Phaseolus vulgaris; Safety; Starch blocker; Toxicity; White kidney bean extract (Phase 2)

Indexed keywords

PHASE 2; PHASEOLUS VULGARIS EXTRACT; PLANT EXTRACT; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BODY WEIGHT; CONTROLLED STUDY; DIET SUPPLEMENTATION; DOSE RESPONSE; DRUG SAFETY; ENZYME INHIBITION; FEMALE; HISTOPATHOLOGY; MALE; MORTALITY; NONHUMAN; NUTRITION; PHASEOLUS VULGARIS; RAT; WEIGHT REDUCTION;

ADMINISTRATION, ORAL; ANIMALS; BODY WEIGHT; DIETARY SUPPLEMENTS; DOSE-RESPONSE RELATIONSHIP, DRUG; FEMALE; MALE; NO-OBSERVED-ADVERSE-EFFECT LEVEL; ORGAN SIZE; PHASEOLUS; PLANT EXTRACTS; RATS;

PHASEOLUS VULGARIS;

EID: 33751243945     PISSN: 02786915     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.fct.2006.06.021     Document Type: Article

References (37)

1. American Association of Cereal Chemists (AACC), 1983. Approved methods of the AACC. Method 71-10. Determination of trypsin inhibitor activity of soy products.
2. American Organization of Analytic Chemists (AOAC), 1995. Moisture analysis 32.1.03. AOAC, 16th ed.
3. Banwell J., Boldt D., Meyers J., and Weber F. Phytohemagglutinin derived from red kidney bean (Phaseolus vulgaris): a cause for intestinal malabsorption associated with bacterial overgrowth in the rat. Gastroenterology 84 3 (1983) 506-515
4. Boivin M. Effect of a purified amylase inhibitor on carbohydrate metabolism after a mixed meal in healthy humans. Mayo Clin. Proc. 62 (1987) 249-255
5. Boivin M., Flourie B., Rizza R.A., Go V.L., and DiMagno E.P. Gastrointestinal and metabolic effects of amylase inhibition in diabetics. Gastroenterology 94 2 (1988) 387-394 (Abstract Only)
6. Bowman D. Amylase inhibitor of navy beans. Science 102 2649 (1945) 358-359
7. Carmalt J., Rosel K., Burns T., and Janzen E. Suspected white kidney bean (Phaseolus vulgaris) toxicity in horses and cattle. Aust. Vet. J. 81 11 (2003) 674-676
8. Cavalle de Moya C., Grant G., Fruhbeck G., Urdaneta E., Garcia M., Marzo F., and Santidrian S. Local (gut) and systemic metabolism of rats is altered by consumption of raw bean (Phaseolus vulgaris L. var. athropurpurea). Br. J. Nutr. 89 3 (2003) 311-318
9. Florida State University (FSU), 2004. Results of Phaseolamin hemagglutinating activity (HA) and trypsin inhibitor activity testing. Department of Nutrition, Food and Exercise Sciences, College of Human Sciences, Tallahassee, Florida (unpublished).
10. Food and Nutrition Board (FNB). Dietary Reference Intakes for Vitamin A, Vitamin K, Arsenic, Boron, Chromium, Copper, Iodine, Iron, Manganese, Molybdenum, Nickel, Silicon, Vanadium, and Zinc (2001), National Academy Press, Washington DC
11. Grant G., Dorward P., Buchan W., Armour J., and Pusztai A. Consumption of diets containing raw soya beans (Glycine max), kidney beans (Phaseolus vulgaris), cowpeas (Vigna, unguiculata) or lupin seeds (Lupinus angustifolius) by rats for up to 700 days effects on body composition and organ weights. Br. J. Nutr. 73 1 (1995) 17-29
12. Green E., and Baenziger J. Oligosaccharide specificities of Phaseolus vulgaris leukoagglutinating and erythroagglutinating phytohemagglutinins: interactions with N-glycanase-released oligosaccharides. J. Biol. Chem. 262 25 (1987) 12018-12029
13. Greer F., Brewer A., and Pusztai A. Effect of kidney bean (Phaseolus vulgaris) toxin on tissue weight and composition and some metabolic functions of rats. Br. J. Nutr. 54 1 (1985) 95-103
14. Haidvogl M., Fritsch G., and Grubauer H. Poisoning by raw garden beans (Phaseolus vulgaris and Phaseolus coccineus) in children. Padiatr. Padol. 14 (1979) 293. http://www.hort.purdue.edu/newcrop/default.html. In: Duke, J.A. (Ed.), Handbook of Energy Crops (unpublished). Available through Purdue University Center for New Crops and Plant Products website:
15. Hara T., Tsukamoto I., and Miyoshi M. Oral toxicity of Kintoki bean (Phaseolus vulgaris) lectin. J. Nutr. Sci. Vitaminol. (Tokyo) 29 5 (1983) 589-599
16. Harikumar K.B., Jesil A.M., Sabu M.C., and Kuttan R. A preliminary assessment of the acute and subchronic toxicity profile of Phase 2: an α-amylase inhibitor. Int. J. Toxicol. 24 (2005) 95-102
17. Hathcock, J.N., 2004. Part 4: Trace Elements Boron. Vitamin and Mineral Safety, second ed., Council for Responsible Nutrition (CRN).
18. Huisman J., van der Poel A., van Leewen P., and Verstegen M. Comparison of growth, nitrogen metabolism and organ weight in piglets and rats fed on diets containing Phaseolus vulgaris beans. Br. J. Nutr. 64 3 (1990) 743-753
19. Kik M., Huisman J., van der Poel A., and Mouwen J. Pathologic changes of the small intestinal mucosa of pigs after feeding Phaseolus vulgaris beans. Vet. Pathol. 27 (1990) 329-334
20. Kneen E., and Sandstedt R. An amylase inhibitor from certain cereals. J. Am. Chem. Soc. 65 (1943) 1247
21. Lajolo F., and Finardi Filho F. Partial characterization of the amylase inhibitor of black beans (Phaseolus vulgaris) variety Rico 23. J. Agric. Food Chem. 33 (1985) 132-138
22. Layer P., Carlson G.L., and DiMagno E.P. Partially Purified White Bean Amylase Inhibitor Reduces Starch Digestion In Vitro and Inactivates Intraduodenal Amylase in Humans (1985), The American Gastroenterological Association
23. Layer P., Zinsmeister A.R., and DiMagno E.P. Effects of decreasing intraluminal amylase activity on starch digestion and postprandial gastrointestinal function in humans. Gastroenterology 91 1 (1986) 41-48
24. Layer P., Rizza R.A., Zinsmeister A.R., Carlson G.L., and DiMagno E.P. Effect of a purified amylase inhibitor on carbohydrate tolerance in normal subjects and patients with diabetes mellitus. Mayo Clin. Proc. 61 6 (1986) 442-447
25. Lis H., and Sharon N. Soy bean (Glycine max) agglutinin. Methods Enzymol. 28 (1972) 360-368
26. Marshall J., and Lauda C. Purification and properties of Phaseolamin, an inhibitor of α-amylase, from the kidney bean, Phaseolus vulgaris. J. Biol. Chem. 250 20 (1975) 8030-8037
27. Myer R., Froseth J., and Coon C. Protein utilization and toxic effects of raw beans (Phaseolus vulgaris) for young pigs. J. Anim. Sci. 55 (1982) 1087-1098
28. de Oliveira A., Vidal Bde C., and Sgarbieri V. Lesions of the intestinal epithelium by ingestion of bean lectins in rats. J. Nutr. Sci. Vitaminol. (Tokyo) 35 4 (1989) 315-322
29. Powers J., and Whitaker J. Purification and some physical and chemical properties of red kidney bean (Phaseolus vulgaris) α-amylase inhibitor. J. Food Biochem. 1 (1977) 217-238
30. Powers J., and Whitaker J. Effect of several experimental parameters on combination of red kidney bean (Phaseolus vulgaris) α-amylase inhibitor with porcine pancreatic α-amylase. J. Food Biochem. 1 (1977) 239-260
31. Pusztai A., King T.P., and Clarke E. Recent advances in the study of the nutritional toxicity of kidney bean (Phaseolus vulgaris) lectins in rats. Toxicon 20 1 (1982) 195-197
32. Rodhouse J., Haugh C., Roberts D., and Gilbert R. Red kidney bean poisoning in the UK: an analysis of 50 suspected incidents between 1976 and 1989. Epidemiol. Infect. 105 3 (1990) 485-491
33. Sockett, P., Cowden, J., Le Baigue, S., Ross, D., Adak, G.K., Evans, H., 1993. Foodborne disease surveillance in England and Wales: 1989-1991. Communicable Disease Report, Volume 3, Review Number 12 (5 November 1993), R168. Public Health Laboratory Service, Communicable Disease Surveillance Centre. Available from: .
34. ® brand proprietary fractioned white bean extract. Alternative Med. Rev. 9 1 (2004) 63-69
35. Uebersax, M.A., 2001. Letter to Pharmachem Laboratories, Inc. from Mark A. Uebersax, Professor and Chairperson, Michigan State University Department of Food Science and Human Nutrition, dated October 9, 2001.
36. United States FDA/CFSAN, 1992. Phytohaemagglutinin (Red kidney bean poisoning). The Bad Bug Book. Foodborne Pathogenic Microorganisms and Natural Toxins Handbook. Available from: .
37. Wilcox E., and Whitaker J. Some aspects of the mechanism of complexation of red kidney bean α-amylase inhibitor and α-amylase. Biochemistry 23 (1984) 1783-1791