The concerted contribution of the S4-S5 linker and the S6 segment to the modulation of a Kv channel by 1-alkanols

Molecular Pharmacology

Volumn 70, Issue 5, 2006, Pages 1542-1554

  Bhattacharji, Aditya ^a   Kaplan, Benjamin ^a   Harris, Thanawath ^a   Qu, Xiaoguang ^b   Germann, Markus W ^b   Covarrubias, Manuel ^a  

^a THOMAS JEFFERSON UNIVERSITY   (United States)

^b GEORGIA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALKANOL; BUTANOL; COMPLEMENTARY DNA; MUTANT PROTEIN; PROTEIN; PROTEIN SHAW2; UNCLASSIFIED DRUG; VOLTAGE GATED POTASSIUM CHANNEL;

ALPHA HELIX; ANIMAL CELL; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; ENZYME ACTIVATION; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN MOTIF; PROTEIN STRUCTURE; XENOPUS LAEVIS;

1-BUTANOL; AMINO ACID SEQUENCE; ANIMALS; CIRCULAR DICHROISM; DIMERIZATION; ION CHANNEL GATING; KINETICS; MOLECULAR SEQUENCE DATA; MUTATION; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PROTEIN STRUCTURE, QUATERNARY; PROTEIN STRUCTURE, SECONDARY; RATS; SHAW POTASSIUM CHANNELS; STRUCTURE-ACTIVITY RELATIONSHIP; THERMODYNAMICS; XENOPUS;

EID: 33751200506     PISSN: 0026895X     EISSN: 15210111     Source Type: Journal    
DOI: 10.1124/mol.106.026187     Document Type: Article

References (39)

1. Anantharam V, Bayley H, Wilson A, and Treistman SN (1992) Differential effects of ethanol on electrical properties of various potassium channels expressed in oocytes. Mol Pharmacol 42:499-505.
2. v4 channels exhibit modulation of closed-state inactivation in inside-out patches. Biophys J 81:867-883.
3. Campagna JA, Miller KW, and Forman SA (2003) Mechanisms of actions of inhaled anesthetics. N Engl J Med 348:2110-2124.
4. Cantor CR and Schimmel PR (1980) Biophysical Chemistry: Part III The Behavior of Biological Macromolecules, W.H. Freeman and Company, San Francisco, CA.
5. + channel, in Basic and Systemic Mechanisms of Anesthesia (Mashimo T, Ogli K, and Uchida I eds) pp 55-60, Elsevier B.V., Amsterdam.
6. Covarrubias M, Vyas TB, Escobar L, and Wei A (1995) Alcohols inhibit a cloned potassium channel at a discrete saturable site. Insights into the molecular basis of general anesthesia. J Biol Chem 270:19408-19416.
7. Covarrubias M, Wei A, Salkoff L, and Vyas TB (1994) Elimination of rapid potassium channel inactivation by phosphorylation of the inactivation gate. Neuron 13:1403-1412.
8. + channel. Neuron 32:649-656.
9. Diamond I and Gordon AS (1997) Cellular and molecular neuroscience of alcoholism. Physiol Rev 77:1-20.
10. Ding S and Horn R (2003) Effect of S6 tail mutations on charge movement in shaker potassium channels. Biophys J 84:295-305.
11. + channel. Biophys J 87:2116-2130.
12. + channel. J Biol Chem 281:12858-12864.
13. + channel. J Gen Physiol 119:521-532.
14. Harris RA (1999) Ethanol actions on multiple ion channels: which are important? Alcohol Clin Exp Res 23:1563-1570.
15. + channel by 1-alkanols is linked to a key residue in the activation gate. Am J Physiol 285:C788-C796.
16. + channel. J Biol Chem 275:4928-4936.
17. Hemmings HC Jr, Akabas MH, Goldstein PA, Trudell JR, Orser BA, and Harrison NL (2005) Emerging molecular mechanisms of general anesthetic action. Trends Pharmacol Sci 26:503-510.
18. Hodge JJ, Choi JC, O'Kane CJ, and Griffith LC (2005) Shaw potassium channel genes in Drosophila. J Neurobiol 63:235-254.
19. v4 potassium channels: molecular interactions involving the inner vestibule of the pore. J Gen Physiology 113:641-660.
20. Kruse SW, Zhao R, Smith DP, and Jones DN (2003) Structure of a specific alcohol-binding site defined by the odorant binding protein LUSH from Drosophila melanogaster. Nat Struct Biol 10:694-700.
21. Labro AJ, Raes AL, Bellens I, Ottschytsch N, and Snyders DJ (2003) Gating of shaker-type channels requires the flexibility of S6 caused by prolines. J Biol Chem 278:50724-50731.
22. Lacroix E, Viguera AR, and Serrano L (1998) Elucidating the folding problem of alpha-helices: local motifs, long-range electrostatics, ionic-strength dependence and prediction of NMR parameters. J Mol Biol 284:173-191.
23. Long SB, Campbell EB, and MacKinnon R (2005) Voltage sensor of Kv1.2: structural basis of electromechanical coupling. Science (Wash DC) 309:903-908.
24. + channels. J Gen Physiol 120:663-676.
25. Muñoz V and Serrano L (1994) Elucidating the folding problem of helical peptides using empirical parameters. Nat Struct Biol 1:399-409.
26. Ohlenschlager O, Hojo H, Ramachandran R, Gorlach M, and Haris PI (2002) Three-dimensional structure of the S4-S5 segment of the shaker potassium channel. Biophys J 82:2995-3002.
27. Peoples RW, Li C, and Weight FF (1996) Lipid vs protein theories of alcohol action in the nervous system. Annu Rev Pharmacol Toxicol 36:185-201.
28. Rettig J, Wunder F, Stocker M, Lichtinghagen R, Mastiaux F, Beckh S, Kues W, Pedarzani P, Schroter KH, Ruppersberg JP, et al. (1992) Characterization of a shaw-related potassium channel family in rat brain. EMBO (Eur Mol Biol Organ) J 11:2473-2486.
29. + channels conserved in flies, mice and humans. Trends Neurosci 15:161-166.
30. Shahidullah M, Harris T, Germann MW, and Covarrubias M (2003) Molecular features of an alcohol binding site in a neuronal potassium channel. Biochemistry 42:11243-11252.
31. Smith-Maxwell CJ, Ledwell JL, and Aldrich RW (1998) Role of the S4 in cooperativity of voltage-dependent potassium channel activation. J Gen Physiol 111:399-420.
32. Sreerama N, Venyaminov SY, and Woody RW (2000) Estimation of protein secondary structure from circular dichroism spectra: inclusion of denatured proteins with native proteins in the analysis. Anal Biochem 287:243-251.
33. Sreerama N and Woody RW (2000) Estimation of protein secondary structure from circular dichroism spectra: comparison of CONTIN, SELCON, and CDSSTR methods with an expanded reference set. Anal Biochem 287:252-260.
34. Sreerama N and Woody RW (2004) On the analysis of membrane protein circular dichroism spectra. Protein Sci 13:100-112.
35. + channels. J Biol Chem 277:18994-19000.
36. Tsunoda S and Salkoff L (1995) Genetic analysis of Drosophila neurons: Shal, Shaw, and Shab encode most embryonic potassium currents. J Neurosci 15:1741-1754.
37. + channels defined by high-affinity metal bridges. Nature (Lond) 428:864-868.
38. Wüthrich K (1986) NMR of Proteins and Nucleic Acids. Wiley-Interscience, New York.
39. + channel. Cell 111:231-239.