Charged nylon membrane substrate for convenient and versatile high resolution microscopic analysis of Escherichia coli & mammalian cells in suspension culture

Cytotechnology

Volumn 51, Issue 2, 2006, Pages 111-117

  Raje, Manoj ^a   Dhiman, Rohan ^a   Majumdar, Sekhar ^a   Dass, Taposh ^b   Dikshit, Kanak L ^a   Kaur, Ramandeep ^a  

^a INSTITUTE OF MICROBIAL TECHNOLOGY   (India)

^b ALL INDIA INSTITUTE OF MEDICAL SCIENCES   (India)

Author keywords

Charged membrane; Electron microscopy; Immobilization; Isolated cells; Processing

Indexed keywords

NYLON;

ARTICLE; ATOMIC FORCE MICROSCOPY; CELL CULTURE; CELL ISOLATION; CELL LABELING; CELL MEMBRANE; CELL SUSPENSION; CELL ULTRASTRUCTURE; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; CYTOCHEMISTRY; ESCHERICHIA COLI; IMMOBILIZED CELL; IMMUNOGOLD LABELING; MAMMAL CELL; MICROSCOPY; NONHUMAN; SCANNING ELECTRON MICROSCOPY; TRANSMISSION ELECTRON MICROSCOPY;

ESCHERICHIA COLI; MAMMALIA;

EID: 33751085413     PISSN: 09209069     EISSN: 15730778     Source Type: Journal    
DOI: 10.1007/s10616-006-9027-2     Document Type: Article

References (12)

1. Dikshit KL, Webster DA (1988) Cloning, characterization and expression of the bacterial globin gene from Vitreoscilla in Escherichia coli. Gene 70:377-386
2. Fletcher M, Floodgate GD (1976) The adhesion of bacteria to solid surfaces. In: Fuller R, Lovelock DW (eds) Microbial ultrastructure the use of the electron microscope. Academic Press, London, pp 101-107
3. Hirsch JG, Fedorko ME (1968) Ultrastructure of human leukocytes after simultaneous fixation with glutaraldehyde and osmium tetroxide and postfixation in uranyl acetate. J Cell Biol 38:615-627
4. Hohenberg H, Mannweiler K, Muller M (1994) High-pressure freezing of cell suspensions in cellulose capillary tubes. J Microsc 175:34-43
5. Inoue T, Osatake H (1988) A new drying method of biological specimens for scanning electron microscopy: the t-butyl alcohol freeze-drying method. Arch Histol Cytol 51:53-59
6. Kai J, Satoh M, Tsukidate K (1999) A new method for preparing electron microscopic specimens of helicobacter pylori. Med Electron Microsc 32:62-65
7. Kaps M, Burkhardt E (1985) An improved method for electron microscopic observation of cerebrospinal fluid cells. Acta Cytol 29:484-486
8. Mazia D, Schatten G, Sale W (1975) Adhesion of cells to surfaces coated with polylysine: applications to electron microscopy. J Cell Biol 66:198-200
9. Morphew MK, Mcintosh JR (2003) The use of filter membranes for high-pressure freezing of cell monolayers. J Microsc 212:21-25
10. Ramandeep, Dikshit KL, Raje M (2001) Optimization of immunogold labelling TEM: an ELISA-based method for rapid and convenient simulation of processing conditions for quantitative detection of antigen. J Histochem Cytochem 49:355-367
11. Tiedemann, Hohenberg, Kollmann (1998) High-pressure freezing of plant cells cultured in cellulose microcapillaries. J Microsc 189:163-171
12. Van De Sandt MM, Herman CJ, Linde J (1985) Immuno-and enzyme-histo/cytochemical analysis of resin sections and cell suspensions: a comphrensive diagnosis of bone marrow on a single aspiration sample. J Microsc 143:205-213