Efficient transfection of primary zebrafish fibroblasts by nucleofection

Cytotechnology

Volumn 51, Issue 2, 2006, Pages 105-110

  Badakov, Rossen ^a   Jaźwińska, Anna ^b  

^a BOSTON CHILDREN S HOSPITAL   (United States)

^b HARVARD MEDICAL SCHOOL   (United States)

Author keywords

Fibroblasts; Primary cells; Transfection; Zebrafish

Indexed keywords

ANTIBIOTIC G 418; DNA; GREEN FLUORESCENT PROTEIN; HISTONE H2B; HYBRID PROTEIN;

ANIMAL CELL; ARTICLE; CELL LINE; CONTROLLED STUDY; DNA TRANSFECTION; ELECTROPORATION; EMBRYO; FIBROBLAST CULTURE; GENE CONSTRUCT; GENE OVEREXPRESSION; GENETIC TRANSFECTION; NONHUMAN; NONVIRAL GENE DELIVERY SYSTEM; PLASMID; PLASMID VECTOR; PROTEIN EXPRESSION; ZEBRA FISH;

DANIO RERIO;

EID: 33751071100     PISSN: 09209069     EISSN: 15730778     Source Type: Journal    
DOI: 10.1007/s10616-006-9018-3     Document Type: Article

References (14)

1. Aluigi M, Fogli M, Curti A, Isidori A, Gruppioni E, Chiodoni C, Colombo MP, Versura P, D'Errico-Grigioni A, Ferri E, Baccarani M, Lemoli RM (2006) Nucleofection is an efficient nonviral transfection technique for human bone marrow-derived mesenchymal stem cells. Stem Cells 24:454-461
2. Berghmans S, Jette C, Langenau D, Hsu K, Stewart R, Look T, Kanki JP (2005) Making waves in cancer research: new models in the zebrafish. Biotechniques 39:227-237
3. Chen JY, You YK, Chen JC, Huang TC, Kuo CM (2005) Organization and promoter analysis of the zebrafish (Danio rerio) interferon gene. DNA Cell Biol 24:641-650
4. Fan L, Moon J, Crodian J, Collodi P (2006) Homologous recombination in zebrafish ES cells. Transgenic Res 15:21-30
5. Goffinet C, Keppler OT (2006) Efficient nonviral gene delivery into primary lymphocytes from rats and mice. Faseb J 20:500-502
6. Gresch O, Engel FB, Nesic D, Tran TT, England HM, Hickman ES, Korner I, Gan L, Chen S, Castro-Obregon S, Hammermann R, Wolf J, Muller-Hartmann H, Nix M, Siebenkotten G, Kraus G, Lun K (2004) New non-viral method for gene transfer into primary cells. Methods 33:151-163
7. Hong Y, Chen S, Gui J, Schartl M (2004) Retention of the developmental pluripotency in medaka embryonic stem cells after gene transfer and long-term drug selection for gene targeting in fish. Transgenic Res 13:41-50
8. Rambabu KM, Rao SH, Rao NM (2005) Efficient expression of transgenes in adult zebrafish by electroporation. BMC Biotechnol 5:29
9. Rupp RA, Snider L, Weintraub H (1994) Xenopus embryos regulate the nuclear localization of XMyoD. Genes Dev 8:1311-1323
10. Siemen H, Nix M, Endl E, Koch P, Itskovitz-Eldor J, Brustle O (2005) Nucleofection of human embryonic stem cells. Stem Cells Dev 14:378-383
11. Smith KR (2002) Gene transfer in higher animals: theoretical considerations and key concepts. J Biotechnol 99:1-22
12. Watanabe M, Shirayoshi Y, Koshimizu U, Hashimoto S, Yonehara S, Eguchi Y, Tsujimoto Y, Nakatsuji N (1997) Gene transfection of mouse primordial germ cells in vitro and analysis of their survival and growth control. Exp Cell Res 230:76-83
13. Zabner J, Fasbender AJ, Moninger T, Poellinger KA, Welsh MJ (1995) Cellular and molecular barriers to gene transfer by a cationic lipid. J Biol Chem 270:18997-19007
14. Zauner W, Brunner S, Buschle M, Ogris M and Wagner E (1999) Differential behaviour of lipid based and polycation based gene transfer systems in transfecting primary human fibroblasts: a potential role of polylysine in nuclear transport. Biochim Biophys Acta 1428:57-67