Characterization and immunogenicity of an apxIA mutant of Actinobacillus pleuropneumoniae

Veterinary Microbiology

Volumn 118, Issue 3-4, 2006, Pages 230-239

  Xu, Fuzhou ^a,b   Chen, Xiaoling ^b   Shi, Aihua ^b   Yang, Bing ^b   Wang, Jinluo ^b   Li, Yongqing ^b   Guo, Xin ^a   Blackall, P J ^c   Yang, Hanchun ^a  

^a AGRO ENVIRONMENTAL PROTECTION INSTITUTE   (China)

^b INSTITUTE OF PLANT AND ENVIRONMENT PROTECTION   (China)

^c DEPARTMENT OF AGRICULTURE AND FISHERIES   (Australia)

Author keywords

Actinobacillus pleuropneumoniae; apxIA gene; Immunogenicity; Mutant

Indexed keywords

BACTERIAL PROTEIN; BACTERIAL TOXIN; BACTERIAL VACCINE; CHLORAMPHENICOL; HEMOLYSIN; LIVE VACCINE;

ACTINOBACILLUS PLEUROPNEUMONIAE; AMINO TERMINAL SEQUENCE; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BACTERIAL STRAIN; BACTERIUM MUTANT; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; FEMALE; GENE CASSETTE; GENE DELETION; GENE INSERTION; HEMOLYSIS; IMMUNOGENICITY; LUNG DISEASE; MOLECULAR WEIGHT; MORTALITY; MOUSE; NONHUMAN; PNEUMONIA; SERODIAGNOSIS; SEROTYPE; SWINE; VACCINATION; VIRUS VIRULENCE;

ACTINOBACILLUS INFECTIONS; ACTINOBACILLUS PLEUROPNEUMONIAE; ANIMALS; BACTERIAL PROTEINS; BACTERIAL VACCINES; BIOLOGICAL ASSAY; FEMALE; HEMOLYSIN PROTEINS; MICE; MICE, INBRED BALB C; MUTATION; NEUTRALIZATION TESTS; PLEUROPNEUMONIA; RANDOM ALLOCATION; SWINE; SWINE DISEASES; VACCINES, ATTENUATED;

ACTINOBACILLUS PLEUROPNEUMONIAE; MYCOPLASMA; SUIDAE;

EID: 33751055738     PISSN: 03781135     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.vetmic.2006.07.013     Document Type: Article

References (19)

1. Bei W., He Q., Yan L., Fang L., Tan Y., Xiao S., Zhou R., Jin M., Guo A., Lv J., Huang H., and Chen H. Construction and characterisation of a live, attenuated apxIICA inactivation mutant of Actinobacillus pleuropneumoniae lacking a drug resistance marker. FEMS Microbiol. Lett. 243 (2005) 21-27
2. Bejerano M., Nisan I., Ludwig A., Goebel W., and Hanski E. Characterization of the C-terminal domain essential for toxic activity of adenylate cyclase toxin. Mol. Microbiol. 31 (1999) 381-392
3. Betsou F., Sebo P., and Guiso N. The C-terminal domain is essential for protective activity of the Bordetella pertussis adenylate cyclase-hemolysin. Infect. Immun. 63 (1995) 3309-3315
4. Blackall P.J., Bowles R., Pahoff J.L., and Smith B.N. Serological characterisation of Actinobacillus pleuropneumoniae isolated from pigs in 1993-1996. Aust. Vet. J. 77 (1999) 39-43
5. Blackall P.J., Klaasen H.L., Bosch H., Kuhnert P., and Frey J. Proposal of a new serovar of Actinobacillus pleuropneumoniae: serovar 15. Vet. Microbiol. 84 (2002) 47-52
6. Chiers K., Overbeke I.V., and Donne E. Detection of Actinobacillus pleuropneumoniae in cultures from nasal and tonsillar swabs of pigs by a PCR assay based on the nucleotide sequence of a dsbE-like gene. Vet. Microbiol. 83 (2001) 147-159
7. Frey J. Virulence in Actinobacillus pleuropneumoniae and RTX toxins. Trends Microbiol. 3 (1995) 257-261
8. Frey J., and Nicolet J. Hemolysin patterns of Actinobacillus pleuropneumoniae. J. Clin. Microbiol. 28 (1990) 232-236
9. Frey J., Haldimann A., and Nicolet J. Sequence analysis and transcription of the apxI operon (hemolysin I) from Actinobacillus pleuropneumoniae. Gene 142 (1994) 97-102
10. Haesebrouck F., van de Kerkhof A., Dom P., Chiers K., and Ducatelle R. Cross-protection between Actinobacillus pleuropneumoniae biotypes-serotypes in pigs. Vet. Microbiol. 52 (1996) 277-284
11. Hannan P.C., Bhogal B.S., and Fish J.P. Tylosin tartrate and tiamutilin effects on experimental piglet pneumonia induced with pneumonic pig lung homogenate containing mycoplasmas, bacteria and viruses. Res. Vet. Sci. 33 (1982) 76-88
12. Inzana T.J., Todd J., Ma J.N., and Veit H. Characterization of a non-hemolytic mutant of Actinobacillus pleuropneumoniae serotype 5: role of the 110 kilodalton hemolysin in virulence and immunoprotection. Microb. Pathog. 10 (1991) 281-296
13. Lally E.T., Hill R.B., Kieba I.R., and Korostoff J. The interaction between RTX toxins and target cells. Trends Microbiol. 7 (1999) 356-361
14. Nicolet J. Actinobacillus pleuropneumoniae. In: Leman A.D., Straw B., Mengeling W.L., D'Allaire S., and Taylor D.J. (Eds). Diseases of Swine (1992), Iowa State University Press, Ames 401-408
15. Prideaux C.T., Pierce L., Krywult J., and Hodgson A.L. Protection of mice against challenge with homologous and heterologous serovars of Actinobacillus pleuropneumoniae after live vaccination. Curr. Microbiol. 37 (1998) 324-332
16. Prideaux C.T., Lenghaus C., Krywult J., and Hodgson A.L.M. Vaccination and protection of pigs against pleuropneumonia with a vaccine strain of Actinobacillus pleuropneumoniae produced by site-specific mutagenesis of the ApxII operon. Infect. Immun. 67 (1999) 1962-1966
17. Seah J.N., Frey J., and Kwang J. The N-terminal domain of RTX toxin ApxI of Actinobacillus pleuropneumoniae elicits protective immunity in mice. Infect. Immun. 70 (2002) 6464-6467
18. Tumamao J.Q., Bowles R.E., van den Bosch H., Klaasen H.L., Fenwick B.W., Storie G.J., and Blackall P.J. Comparison of the efficacy of a subunit and a live streptomycin-dependent porcine pleuropneumonia vaccine. Aust. Vet. J. 82 (2004) 370-374
19. Tumamao J.Q., Bowles R.E., van den Bosch H., Klaasen H.L., Fenwick B.W., and Blackall P.J. An evaluation of the role of antibodies to Actinobacillus pleuropneumoniae serovar 1 and 15 in the protection provided by sub-unit and live streptomycin-dependent pleuropneumonia vaccines. Aust. Vet. J. 82 (2004) 771-778