Changes with age and the effect of recombinant human BMP-2 on proteoglycan and collagen gene expression in rabbit anulus fibrosus cells

Acta Biochimica et Biophysica Sinica

Volumn 38, Issue 11, 2006, Pages 773-779

  Fei, Qin Ming ^a,b,c   Jiang, Xiao Xing ^a   Chen, Tong Yi ^a   Li, Jun ^b,c   Murakami, Hideki ^b,c   Tsai, Kai Jow ^b,c   Hutton, William C ^b,c  

^a FUDAN UNIVERSITY   (China)

^b ATLANTA VA MEDICAL CENTER   (United States)

^c EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Ageing; Anulus fibrosus cells; Bone morphogenetic protein 2

Indexed keywords

A73025; AGGRECAN; BONE MORPHOGENETIC PROTEIN; COLLAGEN TYPE 1; COLLAGEN TYPE 2; GLYCOSAMINOGLYCAN; MESSENGER RNA; RECOMBINANT HUMAN BONE MORPHOGENETIC PROTEIN 2; RECOMBINANT HUMAN BONE MORPHOGENETIC PROTEIN-2; RECOMBINANT PROTEIN; TRANSFORMING GROWTH FACTOR BETA;

AGING; ANIMAL; ARTICLE; BIOSYNTHESIS; CELL CULTURE; CYTOLOGY; GENE EXPRESSION; GENETICS; HUMAN; INTERVERTEBRAL DISK; LUMBAR VERTEBRA; METABOLISM; RABBIT;

AGGRECANS; AGING; ANIMALS; BONE MORPHOGENETIC PROTEINS; CELLS, CULTURED; COLLAGEN TYPE I; COLLAGEN TYPE II; GENE EXPRESSION; GLYCOSAMINOGLYCANS; HUMANS; INTERVERTEBRAL DISK; LUMBAR VERTEBRAE; RABBITS; RECOMBINANT PROTEINS; RNA, MESSENGER; TRANSFORMING GROWTH FACTOR BETA;

ORYCTOLAGUS CUNICULUS;

EID: 33750967668     PISSN: 16729145     EISSN: 17457270     Source Type: Journal    
DOI: 10.1111/j.1745-7270.2006.00230.x     Document Type: Article

References (18)

1. Masuda K, Oegema TR, An HS. Growth factor and treatment of intervertebral disc degeneration. Spine 2004, 29: 2757-2769
2. Thompson JP, Oegema TR, Bradford DS. Stimulation of mature canine intervertebral disc by growth factor. Spine 1991, 16: 253-260
3. Masuda K, Takegami K, An H, Kumano F, Chiba K, Andersson GB, Schmid T et al. Recombinant osteogenic protein-1 upregulates extracellular matrix metabolism by rabbit annulus fibrosus and nucleus pulposus cells cultured in alginate beads. J Orthop Res 2003, 21: 922-930
4. Yoon ST, Kim KS, Li J, Park JS, Akamaru T, Elmer WA, Hutton, WC. The effect of bone morphogenetic protein-2 on rat intervertebral disc cells in vitro. Spine 2003, 28: 1773-1780
5. Li J, Yoon ST, Hutton WC. Effect of bone morphogenetic protein-2 (BMP-2) on matrix production, other BMPs, and BMP receptors in rat intervertebral disc cells. J Spinal Disord Tech 2004, 17: 423-428
6. Okuda S, Myoui A, Ariga K, Nakase T, Yonenobu K, Yoshikawa H. Mechanisms of age-related decline in insulin-like growth factor-I dependent proteoglycan synthesis in rat intervertebral disc cells. Spine 2001, 26: 2421-2426
7. Matsumoto T, An H, Thonar E, Andersson G, Masuda K. Effect of osteogenic protein-1 on the metabolism of proteoglycan of intervertebral disc cells in aging. Orthop Res Soc Trans 2002, 27: 826
8. Kim YJ, Sah RL, Doong JY, Grodzinsky AJ. Fluorometric assay of DNA in cartilage explants using Hoechst 33258. Anal Biochem 1988, 174: 168-176
9. Farndale RW, Buttle DJ, Barrett AJ. Improved quantitation and discrimination of sulphated glycosaminoglycans by use of dimethylmethylene blue. Biochim Biophys Acta 1986, 883: 173-177
10. ΔΔCt method. Methods 2001, 25: 402-408
11. Roughley PJ. Biology of intervertebral disc aging and dedgeneration involvement of the extracellular matrix. Spine 2004, 29: 2692-2699
12. Maeda S, Kokubun S. Changes with age in proteoglycan synthesis in cells cultured in vitro from the inner and outer rabbit annulus fibrosus. Responses to interleukin-1 and interleukin-1 receptor antagonist protein. Spine 2000, 25: 166-169
13. Cs-Szabo G, Juan DR, Turumella V, Masuda K, Thonar E, An H. Changes in mRNA and protein levels of proteoglycans of the annulus fibrosus and nucleus pulposus during intervertebral disc degeneration. Spine 2002, 27: 2212-2219
14. Goudsouzian NM, Aebi M, Alini M. In situ hybridization of collagen types I and II RNA expression in the bovine intervertebral disc: Variation with age. Orthop Res Soc Trans 1999, 24: 814
15. Takae R, Matsunaga S, Origuchi N, Yamamoto T, Morimoto N, Suzuki S, Sakou T. Immunolocalization of bone morphogenetic protein and its receptors in degeneration of intervertebral disc. Spine 1999, 24: 1397-1401
16. Boden SD, Zdeblick TA, Sandhu HS, Heim SE. The use of rhBMP-2 in interbody fusion cages. Definitive evidence of osteoinduction in humans: A preliminary report. Spine 2000, 25: 376-381
17. Muschik M, Schlenzka D, Ritsila V, Tennstedt C, Lewandrowski KU. Experimental anterior spine fusion using bovine bone morphogenetic protein: a study in rabbit. J Orthop Sci 2000, 5: 165-170
18. Kim DJ, Moon SH, Kim H, Kwon, UH, Park, MS, Han KJ, Hahn SB et al. Bone morphogenetic protein-2 facilitates expression of chondrogenic, not osteogenic, phenotype of human intervertebral disc cells. Spine 2003, 28: 2679-2684