Low glucocorticoid receptor (GR), high Dig2 and low Bcl-2 expression in double positive thymocytes of BALB/c mice indicates their endogenous glucocorticoid hormone exposure

Immunobiology

Volumn 211, Issue 10, 2006, Pages 785-796

  Boldizsár, Ferenc ^a   Pálinkás, László ^a   Czömpöly, Tamás ^a   Bartis, Domokos ^a   Németh, Péter ^a   Berki, Timea ^a  

^a UNIVERSITY OF PÉCS   (Hungary)

Author keywords

Bcl 2; Dexamethasone; Dig2; Double positive thymocytes; Glucocorticoid hormone autoregulation; Glucocorticoid receptor

Indexed keywords

CD4 ANTIGEN; CD8 ANTIGEN; CELL PROTEIN; DEXAMETHASONE; GENE PRODUCT; GLUCOCORTICOID; GLUCOCORTICOID RECEPTOR; MESSENGER RNA; MIFEPRISTONE; PROTEIN BCL 2; PROTEIN DIG2; UNCLASSIFIED DRUG;

ANIMAL CELL; APOPTOSIS; ARTICLE; CONCENTRATION RESPONSE; CONTROLLED STUDY; DRUG MEGADOSE; FLOW CYTOMETRY; GENE EXPRESSION; GENE INDUCTION; MOUSE; MOUSE STRAIN; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; REAL TIME POLYMERASE CHAIN REACTION; RECEPTOR UPREGULATION; SINGLE DRUG DOSE; THYMOCYTE;

EID: 33750937390     PISSN: 01712985     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.imbio.2006.06.005     Document Type: Article

References (59)

1. Almawi W.Y., and Melemdjian O.K. Negative regulation of nuclear factor-κB activation and function by glucocorticoids. J. Mol. Endocrinol. 28 (2002) 69-78
2. Almawi W.Y., Melemedjian O.K., and Jaoude M.M. On the link between Bcl-2 family proteins and glucocorticoid-induced apoptosis. J. Leukocyte Biol. 76 (2004) 7-14
3. Antakly T., Thompson E.B., and O'Donnell D. Demonstration of the intracellular localization and up-regulation of glucocorticoid receptor by in situ hybridization and immunocytochemistry. Cancer Res. 49 8 Suppl (1989) 2230s-2234s
4. Ashraf J., Kunapuli S., Chilton D., and Thompson E.B. Cortivazol mediated induction of glucocorticoid receptor messenger ribonucleic acid in wild-type and dexamethasone-resistant human leukemic (CEM) cells. J. Steroid Biochem. Mol. Biol. 38 (1991) 561-568
5. Ashwell J.D., Lu F.W., and Vacchio M.S. Glucocorticoids in T cell development and function. Annu. Rev. Immunol. 18 (2000) 309-345
6. Berg J.M. DNA binding specificity of steroid receptors. Cell 57 (1989) 1065-1068
7. Berki T., Kumanovics G., Kumanovics A., Falus A., Ujhelyi E., and Nemeth P. Production and flow cytometric application of a monoclonal anti-glucocorticoid receptor antibody. J. Immunol. Methods 214 (1998) 19-27
8. Berki T., Grama L., and Németh P. Detection of steroid induced glucocorticoid receptor (GR) translocation and expression with a FITC labeled monoclonal antibody. Cytometry 10 Suppl. (2000) 99
9. Berki T., Pálinkás L., Boldizsár F., and Németh P. Glucocorticoid (GC) sensitivity and GC receptor expression differ in thymocyte subpopulations. Int. Immunol. 14 (2002) 463-469
10. Broome H.E., Yu A.L., Diccianni M., Camitta B.M., Monia B.P., and Dean N.M. Inhibition of Bcl-xL expression sensitizes T-cell acute lymphoblastic leukemia cells to chemotherapeutic drugs. Leukemia Res. 26 (2002) 311-316
11. Buttgereit F., and Scheffold A. Rapid glucocorticoid effects on immune cells. Steroids 67 (2002) 529-534
12. Cohen J.J. Glucocorticoid-induced apoptosis in the thymus. Semin. Immunol. 4 (1992) 363-369
13. Cole T.J., Blendy J.A., Monaghan A.P., Krieglstein K., Schmid W., Aguzzi A., Fantuzzi G., Hummler E., Unsicker K., and Schutz G. Targeted disruption of the glucocorticoid receptor gene blocks adrenergic chromaffin cell development and severely retards lung maturation. Genes Dev. 9 (1995) 1608-1621
14. Cornett L.E., Hiller F.C., Jacobi S.E., Cao W., and McCraw D.W. Identification of a glucocorticoid response element int he rat beta2-adrenergic receptor gene. Mol. Pharmacol. 54 (1998) 1016-1023
15. Cory S. Regulation of lymphocyte survival by the bcl-2 gene family. Annu. Rev. Immunol. 13 (1995) 513-543
16. Distelhorst C.W., and Howard K.J. Evidence from pulse-chase labeling studies that the antiglucocorticoid hormone RU486 stabilizes the nonactivated form of the glucocorticoid receptor in mouse lymphoma cells. J. Steroid Biochem. 36 (1990) 25-31
17. Eisen L.P., Elsasser M.S., and Harmon J.M. Positive regulation of the glucocorticoid receptor in human T-cells sensitive to the cytolytic effects of glucocorticoids. J. Biol. Chem. 263 (1988) 12044-12048
18. Erlacher M., Knoflach M., Stec I.E., Bock G., Wick G., and Wiegers G.J. TCR signaling inhibits glucocorticoid-induced apoptosis in murine thymocytes depending on the stage of development. Eur. J. Immunol. 35 11 (2005) 3287-3296
19. Evans R.M. The steroid and thyroid receptor superfamily. Science 240 (1988) 889-895
20. Godfrey D.I., Purton J.F., Boyd R.L., and Cole T.J. Stress-free T-cell development: glucocorticoids are not obligatory. Immunol. Today 21 (2000) 606-611
21. Hagerty T., Fernandez E., Lynch K., Wang S.S., Morgan W.W., and Strong R.T. Interaction of a glucocorticoid-responsive element with regulatory sequences in the promoter region of the mouse tyrosine hydroxilase gene. J. Neurochem. 78 (2001) 1379-1388
22. He B., Tong T.K., Hiou-Tim F.F., Al-Akad B., Kronenberg H.M., and Karapli A.C. The murine gene encoding parathyroid hormone: genomic organisation, nucleotide sequence and transcriptional regulation. J. Mol. Endocrinol. 29 (2002) 193-203
23. Hines E.R., Collins J.F., Jones M.D., Serey S.H., and Ghishan F.K. Glucocorticoid regulation of the murine PHEX gene. Am. J. Physiol. Renal Physiol. 283 (2002) 356-363
24. Hoeck W., Rusconi S., and Groner B. Down-regulation and phosphorylation of glucocorticoid receptor in cultured cells. J. Biol. Chem. 264 (1989) 14396-14402
25. Hollenberg S.M., Weinberger C., Ong E.S., Cerelli G., Oro A., Lebo R., Thompson E.B., Rosenfeld M.G., and Evans R.M. Primary structure and expression of a functional human glucocorticoid receptor cDNA. Nature 318 (1985) 635-641
26. Imai E., Miner J.N., Mitchell J.A., Yamamoto K.R., and Granner D.K. Glucocorticoid receptor-cAMP response element-binding protein interaction and the response of the phosphoenolpyruvate carboxykinase gene to glucocorticoids. J. Biol. Chem. 268 (1993) 5353-5356
27. Itani O.A., Liu K.Z., Cornish K.L., Campbell J.R., and Thomas C.P. Glucocorticoids stimulate human sgk1 gene expression by activation of a GRE in its 5′ flanking region. Am. J. Physiol. Endocrinol. Metab. 283 (2002) 971-979
28. Jondal M., Pazirandeh A., and Okret S. Different roles for glucocorticoids in thymocyte homeostasis?. Trends Immunol. 25 (2004) 595-600
29. Kraml J., Kolinska J., Sinkora J., Zakostelecka M., Kadlecova L., Hirsova D., and Noskova L. Glucocorticoid agonistic and antagonistic effects of mifepristone and onapristone on thymocyte subset composition and CD26/dipeptidyl peptidase IV activity in infant male rats. J. Steroid Biochem. Mol. Biol. 87 (2003) 85-96
30. Lefebvre P., Danze P.M., Sablonniere B., Richard C., Formstecher P., and Dautrevaux M. Association of the glucocorticoid receptor binding subunit with the 90 K nonsteroid-binding component is stabilized by both steroidal and nonsteroidal antiglucocorticoids in intact cells. Biochemistry 27 (1988) 9186-9194
31. Ma A., Pena J.C., Chang B., Margosian E., Davidson L., Alt F.W., and Thompson C.B. Bclx regulates the survival of double-positive thymocytes. Proc. Natl. Acad. Sci. USA 92 (1995) 4763-4767
32. Mok C.L., Gil-Gomez G., Williams O., Coles M., Taga S., Tolaini M., Norton T., Kioussis D., and Brady H.J. Bad can act as a key regulator of T cell apoptosis and T cell development. J. Exp. Med. 189 (1999) 575-586
33. Nordeen S.K., Boba B.J., and Moyer M.L. Latent agonist activity of the steroid antagonist, RU486, is unmasked in cells treated with activators of protein kinase A. Mol. Endocrinol. 7 (1993) 731-742
34. Oakley R.H., Jewell C.M., Yudt M.R., Bofetiado D.M., and Cidlowski J.A. The dominant negative activity of the human glucocorticoid receptor beta isoform. Specificity and mechanisms of action. J. Biol. Chem. 274 (1999) 27857-27866
35. Okret S., Poellinger L., Dong Y., and Gustafsson J.A. Down-regulation of glucocorticoid receptor mRNA by glucocorticoid hormones and recognition by the receptor of a specific binding sequence within a receptor cDNA clone. Proc. Natl. Acad. Sci. USA 83 (1986) 5899-5903
36. Otto C., Reichardt H.M., and Schutz G. Absence of glucocorticoid receptor-beta in mice. J. Biol. Chem. 272 (1997) 26665-26668
37. Pariante C.M., Pearce B.D., Pisell T.L., Su C., and Miller A.H. The steroid receptor antagonists RU40555 and RU486 activate glucocorticoid receptor translocation and are not excreted by the steroid hormones transporter in L929 cells. J. Endocrinol. 169 (2001) 309-320
38. Pazirandeh A., Xue Y., Rafter I., Sjövall J., Jondal M., and Okret S. Paracrine glucocorticoid activity produced by mouse thymus epithelial cells. FASEB J. 13 (1999) 893-901
39. Pazirandeh A., Xue Y., Prestegaard T., Jondal M., and Okret S. Effects of altered glucocorticoid sensitivity in the T cell lineage on thymocyte and T cell homeostasis. FASEB J. 16 (2002) 727-729
40. Periyasami S., and Sanchez E.R. Antagonism of glucocorticoid receptor transactivity and cell growth inhibition by transforming growth factor-beta through AP-1 mediated transcriptional repression. Int. J. Biochem. Cell Biol. 34 (2002) 1571-1585
41. Purton J.F., Boyd R.L., Cole T.J., and Godfrey D.I. Intrathymic T cell development and selection proceeds normally in the absence of glucocorticoid receptor signaling. Immunity 13 (2000) 179-186
42. Ray A., and Prefontaine K.E. Physical association and functional antagonism between the p65 subunit of transcription factor NF-kappa B and the glucocorticoid receptor. Proc. Natl. Acad. Sci. USA 91 (1994) 752-756
43. Reed J.C. Bcl-2 family proteins. Oncogene 17 (1998) 3225-3236
44. Reichardt H.M. Immunomodulatory activities of glucocorticoids: insights from transgenesis and gene targeting. Curr. Pharm. Des. 10 (2004) 2797-2805
45. Rogatsky I., Hittelman A.B., Pearce D., and Garabedian M.J. Distinct glucocorticoid receptor transcriptional regulatory surfaces mediate the cytotoxic and cytostatic effects of glucocorticoids. Mol. Cell. Biol. 19 (1999) 5036-5049
46. Rosewicz S., McDonald A.R., Maddux B.A., Goldfine I.D., Miesfield R.L., and Logdson C.D. Mechanism of glucocorticoid down-regulation by glucocorticoids. J. Biol. Chem. 263 (1988) 2581-2584
47. Schaaf M.J., and Cidlowski J.A. Molecular mechanisms of glucocorticoid action and resistance. J. Steroid Biochem. Mol. Biol. 83 (2002) 37-48
48. Schaaf J.M., and Cidlowski J.A. Molecular determinants of glucocorticoid receptor mobility in living cells: the importance of ligand affinity. Mol. Cell. Biol. 23 (2003) 1922-1934
49. Smith D.F., and Toft D.O. Steroid receptors and their associated proteins. Mol. Endocrinol. 7 (1993) 4-11
50. Stocklin E., Wissler M., Gouilleux F., and Groner B. Functional interactions between Stat5 and the glucocorticoid receptor. Nature 383 (1996) 726-728
51. Strähle U., Schmidt A., Kelsey G., Stewart A.F., Cole T.J., Schmid W., and Schütz G. At least three promoters direct expression of the mouse glucocorticoid receptor gene. Proc. Natl. Acad. Sci. USA 89 (1992) 6731-6735
52. Tai T.C., Claycomb R., Her S., Bloom A.K., and Wong D.L. Glucocorticoid responsiveness of the rat phenyletanolamine N-methyltransferase gene. Mol. Pharmacol. 61 (2002) 1385-1392
53. Tosa N., Murakami M., Jia W.Y., Yokoyama M., Masunaga T., Iwabuchi C., Inobe M., Iwabuchi K., Miyazaki T., Onoe K., Iwata M., and Uede T. Critical function of T cell death-associated gene 8 in glucocorticoid-induced thymocyte apoptosis. Int. Immunol. 15 (2003) 741-749
54. Vacchio M.S., Papadopoulos V., and Ashwell J.D. Steroid production in the thymus: implication for thymocyte selection. J. Exp. Med. 179 (1994) 1835-1846
55. Vermes I., Haanen C., Steffens-Nakken H., and Reutelingsperger C. A novel assay for apoptosis flow cytometric detection of phosphatidylserine expression on early apoptotic cell using fluorescein labelled Annexin V. J. Immunol. Methods 184 (1995) 39-51
56. Wang Z., Malone M.H., Thomenius M.J., Zhong F., Xu F., and Distelhorst C.W. Dexamethasone-induced gene 2 (dig2) is a novel pro-survival stress gene induced rapidly by diverse apoptotic signals. J. Biol. Chem. 278 (2003) 27053-27058
57. Wiegers G.J., Knoflach M., Bock G., Niederegger H., Dietrich H., Falus A., Boyd R., and Wick G. CD4(+)CD8(+)TCR(low) thymocytes express low levels of glucocorticoid receptors while being sensitive to glucocorticoid-induced apoptosis. Eur. J. Immunol. 31 (2001) 2293-2301
58. Yang-Yen H.F., Chambard J.C., Sun Y.L., Smeal T., Schmidt T.J., Drouin J., and Karin M. Transcriptional interference between c-Jun and the glucocorticoid receptor: mutual inhibition of DNA binding due to direct protein-protein interaction. Cell 62 (1990) 1205-1215
59. Zhang Z., Jones S., Hagood J.S., Fuentes N.L., and Fuller G.M. Stat3 acts as a co-activator of glucocorticoid receptor signaling. J. Biol. Chem. 272 (1997) 30607-30610