Direct crystallographic observation of an acyl-enzyme intermediate in the elastase-catalyzed hydrolysis of a peptidyl ester substrate: Exploiting the "glass transition" in protein dynamics

Bioorganic Chemistry

Volumn 34, Issue 6, 2006, Pages 410-423

  Ding, Xiaochun ^a   Rasmussen, Bjarne F ^a   Petsko, Gregory A ^a   Ringe, Dagmar ^a  

^a BRANDEIS UNIVERSITY   (United States)

Author keywords

Acyl enzyme intermediate; Crystallographic observation; Elastase catalyzed hydrolysis; Glass transition; Peptidyl ester substrate; Protein dynamics

Indexed keywords

ALANINE; ELASTASE; ESTER DERIVATIVE; OXYGEN; PEPTIDE; PROTEIN; SERINE; TRIPEPTIDE;

ARTICLE; BETA SHEET; CARBOXY TERMINAL SEQUENCE; CATALYSIS; CRYSTAL STRUCTURE; CRYSTALLOGRAPHY; ELECTRON; ENZYME SUBSTRATE; EXPERIMENT; GEOMETRY; GLASS TRANSITION TEMPERATURE; HYDROGEN BOND; HYDROLYSIS; HYPOTHESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; TRANSITION TEMPERATURE;

ACYLATION; ANIMALS; CATALYSIS; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; HYDROLYSIS; KINETICS; OLIGOPEPTIDES; PANCREATIC ELASTASE; PHASE TRANSITION; SWINE;

SUIDAE;

EID: 33750725424     PISSN: 00452068     EISSN: 10902120     Source Type: Journal    
DOI: 10.1016/j.bioorg.2006.10.002     Document Type: Article

References (28)

1. Alber T., Petsko G.A., and Tsernoglou D. Nature 263 5575 (1976) 297-300
2. Brunger A.T. J. Mol. Biol. 203 3 (1988) 803-816
3. Ding, X. Studies of the Mechanism of Elastase via X-ray Crystallography. Ph.D. Thesis, Brandeis University (1995).
4. Ding X., Rasmussen B.F., Petsko G.A., and Ringe D. Biochemistry 33 31 (1994) 9285-9293
5. Douzou P., and Petsko G.A. Adv. Protein Chem. 36 (1984) 245-361
6. Gertler A., and Hofmann T. Can. J. Biochem. 48 3 (1970) 384-386
7. Huber R., Kukla D., Bode W., Schwager P., Bartels K., Deisenhofer J., and Steigemann W. J. Mol. Biol. 89 1 (1974) 73-101
8. Hui-Bon-Hoa G., and Douzou P. J. Biol. Chem. 248 13 (1973) 4649-4654
9. Jones T.A., Zou J.Y., Cowan S.W., and Kjeldgaard. Acta. Crystallogr. A. 47 Pt 2 (1991) 110-119
10. Katona G., Wilmouth R.C., Wright P.A., Berglund G.I., Hajdu J., Neutze R., and Schofield C.J. J. Biol. Chem. 277 24 (2002) 21962-21970
11. Lavie A., Allen K.N., Petsko G.A., and Ringe D. Biochemistry 33 18 (1994) 5469-5480
12. Fenn T.D., Ringe D., and Petsko G.A. Biochemistry 34 (2004) 6464-6474
13. Loncharich R.J., and Brooks B.R. J. Mol. Biol. 215 3 (1990) 439-455
14. Neidhart D., Wei Y., Cassidy C., Lin J., Cleland W.W., and Frey P.A. Biochemistry 40 8 (2001) 2439-2447
15. Petsko G.A. J. Mol. Biol. 96 3 (1975) 381-392
16. Petsko G.A. Methods Enzymol. 114 (1985) 141-146
17. Petsko G.A., and Ringe D. Curr. Opin. Chem. Biol. 4 1 (2000) 89-94
18. Polgar L. Cell Mol. Life Sci. 62 19-20 (2005) 2161-2172
19. Rasmussen B.F., Stock A.M., Ringe D., and Petsko G.A. Nature 357 6377 (1992) 423-424
20. Ringe D., and Petsko G.A. Biophys. Chem. 105 2-3 (2003) 667-680
21. Ruhlmann A., Kukla D., Schwager P., Bartels K., and Huber R. J. Mol. Biol. 77 3 (1973) 417-436
22. Schlichting I., Almo S.C., Rapp G., Wilson K., Petratos K., Lentfer A., Wittinghofer A., Kabsch W., Pai E.F., Petsko G.A., and Goody R.S. Nature 345 6273 (1990) 309-315
23. Schlichting I., Berendzen J., Phillips Jr. G.N., and Sweet R.M. Nature 371 6500 (1994) 808-812
24. Schlichting I., Berendzen J., Chu K., Stock A.M., Maves S.A., Benson D.E., Sweet R.M., Ringe D., Petsko G.A., and Sligar S.G. Science 287 5458 (2000) 1615-1622
25. Takahashi L.H., Radhakrishnan R., Rosenfield Jr. R.E., and Meyer Jr. E.F. Biochemistry 28 19 (1989) 7610-7617
26. Tilton R.F., Dewan J.C., and Petsko G.A. Biochemistry 31 (1992) 2469-2481
27. Vitkup D., Ringe D., Petsko G.A., and Karplus M. Nature Struct. Biol. 7 1 (2000) 34-38
28. Wilmouth R.C., Edman K., Neutze R., Wright P.A., Clifton I.J., Schneider T.R., Schofield C.J., and Hajdu J. Nat. Struct. Biol. 8 8 (2001) 689-694