Phototoxicity and fluorotoxicity combine to alter the behavior of neutrophils in fluorescence microscopy based flow adhesion assays

Microscopy Research and Technique

Volumn 69, Issue 11, 2006, Pages 875-884

  Smith, Emily ^a   Lally, Frank ^a   Stone, Michael A ^b   Shaw, John S ^b   Nash, Gerard B ^a   Buckley, Christopher D ^a   Rainger, G Ed ^a  

^a UNIVERSITY DEPARTMENT OF MEDICINE   (United Kingdom)

^b ASTRAZENECA   (United Kingdom)

Author keywords

Cytotoxicity; Fluorescent microscopy; Neutrophil recruitment

Indexed keywords

ADHESION; CELL ADHESION; FLUORESCENCE; FLUORESCENCE MICROSCOPY; PROBES;

ADHESION ASSAYS; ENDOTHELIAL-CELLS; FLUORESCENT DYES; FLUORESCENT MICROSCOPY; HIGH INTENSITY; INTENSITY ILLUMINATION; LEUCOCYTES; NEUTROPHILE RECRUITMENT; PHASE-CONTRAST; PHOTO-TOXICITY;

ENDOTHELIAL CELLS;

FLUORESCENT DYE; FLUOROCHROME; FORMYLMETHIONYLLEUCYLPHENYLALANINE; PADGEM PROTEIN; TUMOR NECROSIS FACTOR ALPHA;

ADULT; ARTICLE; CELL INTERACTION; CONTROLLED STUDY; ENDOTHELIUM CELL; FLUORESCENCE MICROSCOPY; HIGH INTENSITY ILLUMINATION; HUMAN; HUMAN CELL; ILLUMINATION; LEUKOCYTE ADHERENCE; LEUKOCYTE MIGRATION; LIGHT INTENSITY; NEUTROPHIL; NORMAL HUMAN; PHASE CONTRAST MICROSCOPY; PHOTOTOXICITY; PRIORITY JOURNAL;

EID: 33750721721     PISSN: 1059910X     EISSN: 10970029     Source Type: Journal    
DOI: 10.1002/jemt.20362     Document Type: Article

References (46)

1. Abbitt KB, Rainger GE, Nash GB. 2000. Effects of fluorescent dyes on selectin and integrin-mediated stages of adhesion and migration of flowing leukocytes. J Immunol Methods 239:109-119.
2. Adams DH, Nash GB. 1996. Disturbance of leucocyte circulation and adhesion to the endothelium as factors in circulatory pathology. Br J Anaesth 77:17-31.
3. Aubin F. 2003. Mechanisms involved in ultraviolet light-induced immunosuppression. Eur J Dermatol 13:515-523.
4. Bahra P, Rainger GE, Wautier JL, Nguyet-Thin L, Nash GB. 1998. Each step during transendothelial migration of flowing neutrophils is regulated by the stimulatory concentration of tumour necrosis factor-α. Cell Adhes Commun 6:491-501.
5. Brenan M, Parish CR. 1984. Intracellular fluorescent labelling of cells for analysis of lymphocyte migration. J Immunol Methods 74:31-38.
6. Brown M. 2003. PDT: Light at the end of the tunnel? Drug Discov Today 8:767-768.
7. Buckley CD, Doyonnas R, Newton JP, Blystone SD, Brown EJ, Watt SM, Simmons DL. 1996. Identification of α v β 3 as a heterotypic ligand for CD31/PECAM-1. J Cell Sci 109 (Part 2), 437-445.
8. Butcher EC, Scollay RG, Weissman IL. 1980. Direct fluorescent labeling of cells with fluorescein or rhodamine isothiocynate. II. Potential application to studies of lymphocyte migration and maturation. J Immunol Methods 37:109-121.
9. Cooke BM, Usami S, Perry I, Nash GB. 1993. A simplified method for culture of endothelial cells and analysis of adhesion of blood cells under conditions of flow. Microvasc Res 45:33-45.
10. Davies MJ. 2003. Singlet oxygen-mediated damage to proteins and its consequences. Biochem Biophys Res Commun 305:761-770.
11. De Clerck LS, Bridts CH, Mertens AM, Moens MM, Stevens WJ. 1994. Use of fluorescent dyes in the determination of adherence of human leucocytes to endothelial cells and the effect of fluorochromes on cellular function. J Immunol Methods 172:115-124.
12. De Gendt CM, De Clerck LS, Bridts CH, Stevens WJ. 1996. The use of calcein acetomethylester (AM)-labelled polymorphonuclear cells in a polycarbonate filter chemotaxis assay. Clin Chim Acta 249:189-195.
13. Diacovo TG, Roth SJ, Buccola JM, Bainton DF, Springer TA. 1996. Neutrophil rolling, arrest, and transmigration across activated, surface-adherent platelets via sequential action of P-selectin and the β 2-integrin CD11b/CD18. Blood 88:146-157.
14. Droge W. 2002. Free radicals in the physiological control of cell function. Physiol Rev 82:47-95.
15. Durand RE, Olive PL. 1982. Cytotoxicity, Mutagenicity and DNA damage by Hoechst 33342. J Histochem Cytochem 30:111-116.
16. Foldes-Filep E, Filep JG. 1992. Mepacrine inhibits fMLP-induced activation of human neutrophil granulocytes, leukotriene B4 formation, and fMLP binding. J Leukoc Biol 52:545-550.
17. Fraticelli A, Serrano CV, Jr, Bochner BS, Capogrossi MC, Zweier JL. 1996. Hydrogen peroxide and superoxide modulate leukocyte adhesion molecule expression and leukocyte endothelial adhesion. Biochim Biophys Acta 1310:251-259.
18. Gear AR. 1974. Rhodamine 6G. A potent inhibitor of mitochondrial oxidative phosphorylation. J Biol Chem 249:3628-3637.
19. Janssen GH, Tangelder GJ, Oude Egbrink MGA, Reneman RS. 1994. Spontaneous leukocyte rolling in venules in untraumatized skin of conscious and anesthetized animals. Am J Physiol 267:H1199-H1204.
20. Kaina B. 2003. DNA damage-triggered apoptosis: Critical role of DNA repair, double-strand breaks, cell proliferation and signaling. Biochem Pharmacol 66:1547-1554.
21. Lawrence MB, Springer TA. 1991. Leukocytes roll on a selectin at physiologic flow rates: Distinction from and prerequisite for adhesion through integrins. Cell 65:859-873.
22. Loeffler D, Ratner S. 1989. In vivo localization of lymphocytes labelled with low concentrations of Hoechst 33342. J Immunol Methods 119:95-101.
23. Luu NT, Rainger GE, Buckley CD, Nash GB. 2003. CD31 regulates direction and rate of neutrophil migration over and under endothelial cells. J Vasc Res 40:467-479.
24. Melder RJ, Munn LL, Yamada S, Ohkubo C, Jain RK. 1995. Selectin- and integrin-mediated T-lymphocyte rolling and arrest on TNF-α-activated endothelium: Augmentation by erythrocytes. Biophys J 69:2131-2138.
25. Mikes PS, Polomski LL, Gee JB. 1988. Mepacrine impairs neutrophil response after acute lung injury in rats. Effects on neutrophil migration. Am Rev Respir Dis 138:1464-1470.
26. Miller MJ, Wei SH, Parker I, Cahalan MD. 2002. Two-photon imaging of lymphocyte motility and antigen response in intact lymph nodes. Science 296:1869-1873.
27. Muller W, Gautier F. 1975. Interactions of heteroaromatic compounds with nucleic acids. A-T-specific non-intercalating DNA ligands. Eur J Biochem 54:385-394.
28. Neal TM, Vissers MC, Winterbourn CC. 1987. Inhibition by nonsteroidal anti-inflammatory drugs of superoxide production and granule enzyme release by polymorphonuclear leukocytes stimulated with immune complexes or formyl-methionyl-leucyl-phenylalanine. Biochem Pharmacol 36:2511-2517.
29. Nolte D, Schmid P, Jager U, Botzlar A, Roesken F, Hecht R, Uhl E, Messmer K, Vestweber D. 1994. Leukocyte rolling in venules of striated muscle and skin is mediated by P-selectin, not by L-selectin. Am J Physiol 267,H1637-H1642.
30. Radcliff G, Waite R, LeFevre J, Poulik MD, Callewaert DM. 1991. Quantification of effector/target conjugation involving natural killer (NK) of lymphokine activated killer (LAK) cells by two-color flow cytometry. J Immunol Methods 139:281-292.
31. Rainger GE, Nash GB. 2001. Cellular pathology of atherosclerosis: Smooth muscle cells prime cocultured endothelial cells for enhanced leukocyte adhesion. Circ Res 88:615-622.
32. Rainger GE, Fisher A, Shearman C, Nash GB. 1995. Adhesion of flowing neutrophils to cultured endothelial cells after hypoxia and reoxygenation in vitro. Am J Physiol 269:H1398-H1406.
33. Rainger GE, Buckley C, Simmons DL, Nash GB. 1997. Cross-talk between cell adhesion molecules regulates the migration velocity of neutrophils. Curr Biol 7:316-325.
34. Rainger GE, Stone P, Morland CM, Nash GB. 2001. A novel system for investigating the ability of smooth muscle cells and fibroblasts to regulate adhesion of flowing leukocytes to endothelial cells. J Immunol Methods 255:73-82.
35. Read NG, Trist DG. 1982. The uptake of mepacrine by horse polymorphonuclear leucocytes in vitro. J Pharm Pharmacol 34:711-714.
36. Saetzler RK, Jallo J, Lehr HA, Philips CM, Vasthare U, Arfors KE, Tuma RF. 1997. Intravital fluorescence microscopy: Impact of light-induced phototoxicity on adhesion of fluorescently labeled leukocytes. J Histochem Cytochem 45:505-513.
37. Samlowski WE, Robertson BA, Draper BK, Prystas E, McGregor JR. 1991. Effects of supravital fluorochromes used to analyze the in vivo homing of murine lymphocytes on cellular function. J Immunol Methods 144:101-115.
38. Sheikh S, Nash GB. 1996. Continuous activation and deactivation of integrin CD11b/CD18 during de novo expression enables rolling neutrophils to immobilize on platelets. Blood 87:5040-5050.
39. Soper SA, Nutter HL, Keller RA, Davis LM, Shera EB. 1993. The photophysical constants of several fluorescent dyes pertaining to ultrasensitive fluorescence spectroscopy. Photochem Photobiol 57:972-977.
40. Springer TA. 1995. Traffic signals on endothelium for lymphocyte recirculation and leukocyte emigration. Annu Rev Physiol 57:827-872.
41. Steinbauer M, Harris AG, Abels C, Messmer K. 2000. Characterization and prevention of phototoxic effects in intravital fluorescence microscopy in the hamster dorsal skinfold model. Langenbecks Arch Surg 385:290-298.
42. Sumner AT. 1982. Application of X-ray microanalysis to the study of histochemical staining reactions. Scan Electron Microsc (Part 1), 261-268.
43. Vaporciyan AA, Jones ML, Ward PA. 1993. Rapid analysis of leukocyte-endothelial adhesion. J Immunol Methods 159:93-100.
44. Wang J, Xing D, He Y, Hu X. 2002. Experimental study on photodynamic diagnosis of cancer mediated by chemiluminescence probe. FEBS Lett 523:128-132.
45. West CA, He C, Su M, Swanson SJ, Mentzer SJ. 2001. Aldehyde fixation of thiol-reactive fluorescent cytoplasmic probes for tracking cell migration. J Histochem Cytochem 49:511-518.
46. Weston SA, Parish CR. 1990. New fluorescent dyes for lymphocyte migration studies. Analysis by flow cytometry and fluorescence microscopy. J Immunol Methods 133:87-97.