Tetrahymena eukaryotic translation elongation factor 1A (eEF1A) bundles filamentous actin through dimer formation

Journal of Biochemistry

Volumn 140, Issue 3, 2006, Pages 393-399

  Bunai, Fumihide ^a   Ando, Kunie ^a   Ueno, Hironori ^a   Numata, Osamu ^a  

^a UNIVERSITY OF TSUKUBA   (Japan)

Author keywords

Actin; Ca2+; Calmodulin; Cytoskeleton; Eukaryotic translation elongation factor 1A (eEF1A); Tetrahymena

Indexed keywords

CALCIUM; CALMODULIN; ELONGATION FACTOR 1ALPHA; F ACTIN;

ACTIN FILAMENT; ARTICLE; CELL CULTURE; CONTROLLED STUDY; CYTOSKELETON; DIMERIZATION; ELECTRON MICROSCOPY; ELECTROPHORESIS; GEL FILTRATION CHROMATOGRAPHY; IMMUNOBLOTTING; NONHUMAN; PROTEIN BINDING; TETRAHYMENA; TRANSLATION INITIATION; TRANSLATION REGULATION;

ACTINS; ANIMALS; CALMODULIN; CHROMATOGRAPHY, GEL; DIMERIZATION; ELECTROPHORESIS, POLYACRYLAMIDE GEL; IMMUNOBLOTTING; MICROSCOPY, ELECTRON; PEPTIDE ELONGATION FACTOR 1; TETRAHYMENA;

EUKARYOTA; TETRAHYMENA;

EID: 33750695976     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/jb/mvj169     Document Type: Article

References (50)

1. Schoeder, T.E. (1968) Cytokinesis: Filamentous in the cleavage furrow. Exp. Cell Res. 53, 272-276
2. Robinson, D.N., and Spudich., J.A. (2000) Towards a molecular understanding of cytokinesis. Trends Cell Biol. 10, 228-237
3. Straight, A.F., and Field, C.M. (2000) Microtubules, membranes and cytokinesis. Curr. Biol. 10, R760-R770
4. Fujiwara, K., and Pollard, T.D. (1976) Fluorescents antibody localization of myosin in the cytoplasm, cleavage furrow, and mitotic spindle of human cells. J. Cell Biol. 71, 848-875
5. Mabuchi, I., Hamaguchi, Y., Fujimoto, H., Morii, N., Mishima, M., and Narumiya, S. (1993) A rho-like protein is involved in the organization of the contractile ring in dividing sand dollar eggs. Zygote 1, 325-331
6. Nunnally, M.H., Dangelo, J.M., and Craig, S.W. (1980) Filamin concentration in the cleavage furrow and midbody region: Frequency of occurrence compared with that of alpha-actinin and myosin. J. Cell Biol. 87, 219-226
7. Mabuchi, L, Hamaguchi, Y., Kobayashi, T., Hosoya, H., Tsukita, S., and Tsukita, S. (1985) Alpha-actinin from sea urchin eggs: Biochemical properties, interaction with actin, and distribution in the cell during fertilization and cleavage. J. Cell Biol. 100, 375-383
8. Field, C.M., and Alberts, B.M. (1995) Anillin, a contractile ring protein that cycles from the nucleus to the cell cortex. J. Cell Biol. 131, 165-178
9. Nagaoka, R., Abe, H., Kusano, K., and Obinata, T. (1995) Concentration of cofilin, a small actin-binding protein, at the cleavage furrow during cytokinesis. Cell Motil. Cytoskelton 30, 1-7
10. Yasuda, T., Numata, O., Ohnishi, K., and Watanabe, Y. (1980) A contractile ring and cortical changes found in the dividing Tetrahymena pyriformis. Exp. Cell Res. 128, 407-418
11. Yasuda, T., Tamura, R., and Watanabe, Y. (1984) Molecular mechanism of cell division in Tetrahymena thermophila. Ultra-structure changes found in a division arrest ts-mutant. Zool. Sci. 1, 62-73
12. Hirono, M., Nakamura, M., Tsunemoto, M., Yasuda, T., Ohba, H., Numata, O., and Watanabe, Y. (1987) Localization and possible biological roles of actin in Tetrahymena calls. J. Biochem. 102, 537-545
13. Watanabe, Y., Ohba, H., Hirono, M., and Yasuda. T. (1990) Analysis of furrow formation and furrowing during cell division in Tetrahymena using cell-division arrest mutants. Ann. N.Y. Acad. Sci. 582, 166-178
14. Edamatsu, M., Hirono, M., and Watanabe, Y. (1992) Tetrahymena profilin is localized in the division furrow. J. Biochem. 112, 637-642
15. 2+/Calmodulin and p85 cooperatively regulate an initiation of cytokinesis in Tetrahymena. J. Cell Sci. 112, 3619-3626
16. Numata, O., Kurasawa, Y., Gronda, K., and Watanabe, Y. (2000) Tetrahymena elongation factor-1α is localized with calmodulin in the division furrow. J. Biochem. 127, 51-56
17. Watanabe, A., kurasawa, Y., Watanabe, Y., and Numata, O. (1998) A new Tetrahymena actin binding protein is localized in the division furrow. J. Biochem. 123, 607-613
18. Shirayama, S., and Numata O., (2003) Tetrahymena fimbrin localized in the division furrow bundles actin filaments in a calcium-independent manner. J. Biochem. 134, 591-598
19. Kaziro, Y. (1978) The role of guanosine 5′-triphosphate in polypeptide chain elongation. Biochim. Biophys. Acta 505, 95-127
20. Moldave, K. (1985) Eukaryotic protein synthesis. Annu. Rev. Biochem. 54, 1109-1149
21. Riis, B., Rattan, S.I.S., Clark, B.F.C., and Merrick, W.C. (1990) Eukaryotic protein elongation factors. Trends Biochem. Sci. 15, 420-424
22. Kuriyama, R., and Borisy, G.G. (1985) Identification of molecular components of the centrosphere in the mitotic spindle of sea urchin eggs. J. Cell Biol. 101, 524-530
23. Kuriyama, R., Saverade, P., Lefebvre, P., and Dasgupta, S. (1990) The predict amino acid sequence of a centrosphere protein in dividing sea urchin eggs is similar to elongation factor (EF-1α). J. Cell Sci. 95, 231-236
24. Ohta, K., Toriyama, M., Endo, S., and Sakai, H. (1988) Mitotic apparatus-associated 51-kD protein in mitosis of sea urchin eggs. Zool. Sci. 5, 613-621
25. Ohta, K., Toriyama, M., Miyazaki, M., Murofushi, H., Hosoda, S., Endo, S., and Sakai, H. (1990) The mitotic apparatus-associated 51-kD protein from sea urchin eggs is a GTP-binding protein and is immunologically related to yeast polypeptide elongation factor 1α. J. Biol. Chem. 265, 3240-3247
26. Shiina, N., Gotoh, Y., Kubomura, N., Iwamatsu, A., and Nishida, E. (1994) Microtubule severing by elongation factor 1α. Science 266, 282-285
27. Demma, M., Warren, V., Hock, R., Dharmawardhane, S., and Condeelis, J. (1990) Isolation of an abundant 50,000-dalton actin filament bundling protein from Dictyostelium amoebae. J. Biol. Chem. 265, 2282-2291
28. Yang, F., Demma, M., Warren, V., Dharmawardhance, S., and Condeelis, J. (1990) Identification of an actin-binding protein from Dictyostelium as elongation factor 1α. Nature 347, 494-496
29. Dharmawardhance, S., Demma, M., Yang, F., and Condeelis, J. (1991) Compartmentalization and actin binding properties of ABP-50: The elongation factor-1 alpha of Dictyostelium. Cell Motil. Cytoskeleton 20, 279-288
30. Itano, N., and Hatano, T. (1991) F-actin bundling protein from Physarum polycephalum: Purification and its capacity for co-bundling of actin filaments and microtubules. Cell Motil. Cytoskeleton 19, 244-254
31. Liu, G., Grant, W.M., Persky, D., Lathan, V.M. Jr.,Singer, R.H., and Condeelis, J. (2002) Identifications of elongation factor 1α with F-actin and β-actin mRNA: Implications for anchoring mRNA in cell protrusions. Mol. Biol. Cell 13, 579-592
32. Edmonds, B.T., Murray, J.W., and Condeelis, J. (1995) pH regulation of the F-actin binding properties of Dictyostelium elongation factor 1α. J. Biol. Chem. 270, 15222-15230
33. Edmonds, B.T., Bell, A., Wyckoff, J., Condeelis, J., and Leth, T.S. (1998) The effect of F-actin on the binding and hydrolysis of guanine nucleotide Dictyostelium elongation factor 1A. J. Biol. Chem. 273, 10288-10295
34. Liu, G., Tang, J., Edmonds, B.T., Murray, J., Levin, S., and Condeelis, J. (1996) F-actin sequesters elongation factor 1α from interaction with aminoacyl-tRNA in a pH-dependent reaction. J. Cell Biol. 135, 953-963
35. Kurasawa, Y., Watanabe, Y., and Numata, O. (1996) Characterization of F-actin bundling activity of Tetrahymena elongation factor-1α investigated with rabbit skeletal muscle actin. Zool. Sci. 13, 371-375
36. 2+/calmodulin. J. Biochem. 119, 791-798
37. Heper, P.K. (1994) The role of calcium in the cell division. Cell Calcium 16, 322-330
38. Briggs M.W., and Sacks D.B. (2003) IQGAP proteins are integral components of cytoskeletal regulation. EMBO Rep. 4, 571-574
39. Watanabe, Y., Numata, O., Kurasawa, Y., and Katoh, M. (1994) Cultivation of Tetrahymena cells. In Cell Biology (Celis, J.E., ed.) pp. 398-404, Academic Press, San Diego
40. Laemmli, U.K. (1970) Cleavage of structure proteins dividing the assembly of the head of bacteriophage T4. Nature 227, 680-685
41. Towbin, H.T., Stahelin, H.M., and Gordon, J. (1979) Electropholetic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76, 4350-4354
42. Takeda, T., Kurasawa, Y., Watanabe, Y., and Numata, O. (1995) Polymerization of highly purified Tetrahymena 14-nm filament protein/citrate synthase into filaments and its possible role in regulation of enzymatic activity. J. Biochem. 117, 869-874
43. 2+-binding protein, TCBP-25, in the cell cortex and possible involvement in conjugation. Exp. Cell Res. 219, 487-493
44. Pardee, J.D. and Spudich, J.A. (1982) Purification of muscle actin. Methods Enzymol. 85, 164-181
45. Pelham, R.J. and Chang, F. (2002) Actin dynamics in the contractile ring during cytokinesis in fission yeast. Nature 419, 82-86
46. Narumiya, S. and Mabuchi, I. (2002) Cell biology: spinning actin to divide. Nature 419, 27-28
47. Kaji, N., Ohashi, K., Shuin, M., Niwa, R., Uemura, T., and Mizuno, K. (2003) Cell cycle-associated changes in Slingshot phosphatase activity and roles in cytokinesis in animal cells. J. Biol Chem. 278, 33450-33455
48. Kovar, D.R., Kuhn, J.R., Tichy, A.L., and Pollard, T.D. (2003) The fission yeast cytokinesis formin Cdc12p is a barbed end actin filament capping protein gated by profilin. J. Cell Biol. 161, 875-887
49. + exchange and growth factore-induced cytosolic pH changes. Role in cellular proliferation. Biochim. Biophy. Acta 988, 73-97
50. Aerts, R.J., Durston, A.J., and Moolenaar, W.H. (1985) Cytoplasmic pH and the regulation of the Dictyosterium cell cycle. Cell 43, 653-657