Reduced irradiation pulmonary fibrosis and stromal cell migration in Smad3-/-marrow chimeric mice

In Vivo

Volumn 20, Issue 5, 2006, Pages 573-582

  Epperly, Michael W ^a   Franicola, Darcy ^a   Zhang, Xichen ^a   Nie, Suhua ^a   Wang, Hong ^a   Bahnson, Alfred B ^b   Shields, Donna S ^a   Goff, Julie P ^a   Shen, Hongmei ^c   Greenberger, Joel S ^a,c  

^a UNIVERSITY OF PITTSBURGH CANCER INSTITUTE   (United States)

^b Automated Cell Inc   (United States)

^c UNIVERSITY OF PITTSBURGH MEDICAL CENTER   (United States)

Author keywords

Bone marrow stromal cells; Gene transfer; Hematopoietic microenvironment; Smad3; TGF signal transduction

Indexed keywords

DYE; GREEN FLUORESCENT PROTEIN; SMAD3 PROTEIN;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BONE MARROW CULTURE; CELL LABELING; CELL LINE; CELL MIGRATION; CELL PROLIFERATION; CHIMERA; CLONAL VARIATION; CONTROLLED STUDY; CORRELATION ANALYSIS; FEMALE; GENE DELETION; GENETIC RECOMBINATION; GENETIC TRANSFECTION; HOMOZYGOSITY; IN VITRO STUDY; LUNG FIBROSIS; MOUSE; MOUSE STRAIN; NONHUMAN; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; RADIATION DOSE; RADIATION INJURY; RETROVIRUS VECTOR; STROMA CELL; SURVIVAL RATE; TRANSGENIC MOUSE; WHOLE BODY RADIATION;

EID: 33750602761     PISSN: 0258851X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (24)

1. Leask A and Abraham DJ: TGF-β signaling and the fibrotic response. FASEB J 18: 816-827, 2004.
2. Anscher MS, Peters WP and Reisenbichler H: Transforming growth factor β as a predictor of liver and lung fibrosis after autologous bone marrow transplantation for advanced breast cancer. N Engl J Med 328: 1592-1598, 1993.
3. Kelley TJ, Elmer HL and Corey DA: Reduced Smad3 protein expression and altered transforming growth factor-beta 1-mediated signaling in cystic fibrosis epithelial cells. Am J Respir Cell Mol Biol 25: 732-738, 2001.
4. Reisdorf P, Lawrence DA, Sivan V, Ktising E and Martin MT: Alteration of transforming growth factor-beta 1 response involves down-regulation of Smad3 signaling in myofibroblasts from skin fibrosis. Am J Pathol 59: 263-272, 2001.
5. Massague J and Wotton D: Transcriptional control by the TGF-β/Smad signaling system. EMBO J 19: 1745-1754, 2000.
6. Epperly MW, Sikora CA, Defilippi S, Gretton JE and Greenberger JS: Bone marrow origin of myofibroblasts in irradiation pulmonary fibrosis. Am J Respir Cell Mol Biol 29: 213-224, 2003.
7. Ortiz LA, Gambelli F, McBride C et al: Mesenchymal stem cell engraftment is enhanced in response to bleomycin exposure and ameliorates its fibrotic effects. Proc Natl Acad Sci USA 100: 8407-8411, 2003.
8. Hashimoto N, Jin H, Liu T, Chensue SW and Phan SH: Bone marrow-derived progenitor cells in pulmonary fibrosis. J Clin Invest 113: 243-251, 2004.
9. Flanders KC, Sullivan CD and Fujii M: Mice lacking Smad3 are protected against cutaneous injury induced by ionizing radiation. Am J Pathol 160: 1057-1068, 2002.
10. Flanders KC, Major CD and Arabshahi A: Interference with transforming growth factor-beta/Smad3 signaling results in accelerated healing of wounds in previously irradiated skin. Am J Pathol 163: 2247-2257, 2003.
11. Yang X, Chen L, Xu Y, Li C, Huang C and Deng C: TGF-β/Smad3 signals repress chondrocyte hypertrophic differentiation and are required for maintaining articular cartilage. J Cell Biol 153: 35-46, 2001.
12. Yang X, Letterio JJ, Lechieider RJ, Chen L, Hayman R, Gu H, Roberts AB and Deng C: Targeted disruption of Smad3 results in impaired mucosal immunity and diminished T-cell responsiveness to TGF-β. EMBO J 18: 1280-1291, 1999.
13. Epperly MW, Cao S, Goff JP, Shields DS, Zhou S, Glowacki J and Greenberger JS: Increased longevity of hematopoiesis in continuous bone marrow cultures and adipocytogenesis in marrow stromal cells derived from Smad3-/- mice. Exp Hematol 33: 353-362, 2005.
14. Zhou S, Lechpammer S, Greenberger JS and Glowacki J: Hypoxia inhibition of adipocytogenesis in human bone marrow stromal cells requires TGFβ/ Smad3 signaling. J Biol Chem 280: 22688-22696, 2005.
15. Epperly MW, Goff JP, Zhang Y, Shields D, Wang H, Shen H, Franicola D, Bahnson A, Greenberger EE and Greenberger JS: Increased radioresistance, G2M checkpoint inhibition and impaired migratory capacity of bone marrow stromal cell lines derived from Smad3-/- mice. Radiat Res 165: 671-677, 2006.
16. Epperly MW, Travis EL, Sikora C and Greenberger JS: Magnesium superoxide dismutase (MnSOD) plasmid/liposome pulmonary radioprotective gene therapy: Modulation of irradiation-induced mRNA for IL-1, TNF-α, and TGF-β correlates with delay of organizing alveolitis/fibrosis. Biol Blood Marrow Transplant 5: 204-214, 1999.
17. Bahnson A, Athanassiou C, Koebler D, Qian L, Shun T, Shields D, Yu H, Wang H, Goff J, Cheng T, Houck R and Cowsert L: Automated measurement of cell motility and proliferation. BMC Cell Biol 6: 1471-2121, 2005.
18. Epperly MW, Bray JA, Carlos TM, Prochownik E and Greenberger JS: Biology of marrow stromal cell lines derived from long-term bone marrow cultures of Trp53-deficient mice. Radiat Res 152: 29-40, 1999.
19. Epperly MW, Sikora CA, Defilippi S, Gretton JE, Zhan Q, Kufe DW and Greenberger JS: MnSOD inhibits irradiation-induced apoptosis by stabilization of the mitochondrial membrane against the effects of SAP kinases p38 and Jnk1 translocation. Radiat Res 157. 568-577, 2002.
20. FitzGerald TJ, Henault S, Sakakeeny MA, Santucci MA, Pierce JH, Anklesaria P, Kase & Lovington K and Greenberger JS: Resistance of hematopoietic stem ceUs to x-irradiation at clinical low-dose-rate is induced by several classes of oncogenes. Radiat Res 122: 44-53, 1990.
21. Feng XH, Liang YY, Liang M, Zhai W and Lin X: Direct interaction of c-Myc with Smad3 to inhibit TGF-beta-mediated induction of the CDK inhibitor p15(Ink4B). Mol Cell 9: 133-143, 2002.
22. Pardali & Kowanetz M, Heldin CH and Moustakas A: Smad pathway-specific transcriptional regulation of the cell cycle inhibitor p2l(WAFl/Cipl). J Cell Physiol 204: 260-272, 2005.
23. Denicourt C and Dowdy SF: Another twist in the transforming growth factor beta-induced cell cycle arrest chronicle. Proc Natl Acad Sci USA 100: 15290-15291, 2003.
24. Dileto, CL and Travis EL- Fibroblast radiosensitivity in vitro and lung fibrosis in vivo: comparison between a fibrosis-prone and fibrosis-resistant mouse strain. Radiat Res 146: 61-67, 1996.