Microsporidial keratitis in India: 16s rRNA gene-based PCR assay for diagnosis and species identification of microsporidia in clinical samples

Investigative Ophthalmology and Visual Science

Volumn 47, Issue 10, 2006, Pages 4468-4473

  Joseph, Joveeta ^a   Sharma, Savitri ^a   Murthy, Somasheila I ^a   Krishna, Pravin V ^a   Garg, Prashant ^a   Nutheti, Rishita ^a   Kenneth, John ^a   Balasubramanian, Dorairajan ^a  

^a L V PRASAD EYE INSTITUTE   (India)

Author keywords

[No Author keywords available]

Indexed keywords

RNA 16S; FUNGAL DNA; FUNGAL RNA; PRIMER DNA;

ADOLESCENT; ADULT; AGED; AMPLICON; ARTICLE; CHILD; CONTROLLED STUDY; DIAGNOSTIC TEST; ENCEPHALITOZOON; ENCEPHALITOZOON CUNICULI; ENCEPHALITOZOON HELLEM; ENCEPHALITOZOON INTESTINALIS; FEMALE; GENE SEQUENCE; HUMAN; HUMAN CELL; INDIA; KERATITIS; MALE; MICROSPORIDA; NONHUMAN; PARASITE IDENTIFICATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SEQUENCE ANALYSIS; SPECIES IDENTIFICATION; VITTAFORMA; VITTAFORMA CORNEAE; CHEMISTRY; CLASSIFICATION; CORNEA; CORNEA ULCER; FUNGAL EYE INFECTION; GENETICS; ISOLATION AND PURIFICATION; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; MICROSPORIDIOSIS; MIDDLE AGED; PRESCHOOL CHILD;

ADOLESCENT; ADULT; AGED; CHILD, PRESCHOOL; CORNEA; CORNEAL ULCER; DNA PRIMERS; DNA, FUNGAL; ENCEPHALITOZOON; EYE INFECTIONS, FUNGAL; FEMALE; HUMANS; INDIA; MALE; MICROSPORIDIA; MICROSPORIDIOSIS; MIDDLE AGED; MYCOLOGICAL TYPING TECHNIQUES; POLYMERASE CHAIN REACTION; RNA, FUNGAL; RNA, RIBOSOMAL, 16S;

EID: 33750602020     PISSN: 01460404     EISSN: None     Source Type: Journal    
DOI: 10.1167/iovs.06-0376     Document Type: Article

References (20)

1. Franzen C, Muller A. Molecular techniques for detection, species differentiation, and phylogenetic analysis of microsporidia. Clin Microbiol Rev. 1999;12:243-285.
2. Vemuganti GK, Garg P, Sharma S, Joseph J, Gopinathan U, Singh S. Is microsporidial keratitis an emerging cause of stromal keratitis?-a case series study. BMC Ophthalmol. 2005;17;5:19.
3. Joseph J, Sridhar MS, Murthy S, Sharma S. Clinical and microbiological profile of microsporidial keratoconjunctivitis in Southern India. Ophthalmology. 2006;113:531-537.
4. Garcia LS. Laboratory identification of the microsporidia. J Clin Microbiol 2002;40:1892-1901.
5. Joseph J, Murthy S, Garg P, Sharma S. Microscopic evaluation of corneal scrapings using different stains for the diagnosis of microsporidial keratitis. J Clin Microbiol. 2006;22:583-585.
6. Aldras AM, Orenstein JM, Kotler DP, Shadduck JA, Didier ES. Detection of microsporidia by indirect immunofluorescence antibody test using polyclonal and monoclonal antibodies. J Clin Microbiol 1994;32:608-612.
7. Moura H, Sodre FC, Bornay-Llinares FJ, et al. Detection by an immunofluorescence test of Encephalitozoon intestinalis spores in routinely formalin-fixed stool samples stored at room temperature. J Clin Microbiol. 1999;37:2317-2322.
8. Fedorko DP, Nelson NA, Cartwright CP. Identification of microsporidia in stool specimens by using PCR and restriction endonucleases. J Clin Microbiol. 1995;33:1739-1741.
9. Kock NP, Petersen H, Fenner T, et al. Species-specific identification of microsporidia in stool and intestinal biopsy specimens by the polymerase chain reaction. Eur J Clin Microbiol Infect Dis. 1997;16:369-376.
10. Muller A, Stellermann K, Hartmann P, et al. A powerful DNA extraction method and PCR for detection of microsporidia in clinical stool specimens. Clin Diagn Lab Immunol 1999;6:243-246.
11. Kunimoto DY, Sharma S, Garg P, Gopinathan U, Miller D, Rao GN. Corneal ulceration in the elderly in Hyderabad, South India. Br J Opthalmol. 2000;84:54-59.
12. Thiel MA, Bossart W, Bernauer W. Improved impression cytology techniques for the immunopathological diagnosis of superficial viral infections. Br J Ophthalmology. 1997;81:984-988.
13. Farhatullah S, Kaza S, Athmanattian S, et aL Diagnosis of herpes simplex virus-1 keratitis using Giemsa stain, immunofluorescence assay, and polymerase chain reaction assay on corneal scrapings. Br J Ophthalmol. 2004;88:142-144.
14. Asubel FM, Brent R, Kingston RE, et al. Current Protocols in Molecular Biology. Vol 2. New York; John Wiley and Sons; 1990: A1.5
15. Behzadbehbahani A, Klapper PE, Valley PJ, Cleator GM. Detection of BK virus in urine by polymerase chain reaction: a comparison of DNA extraction methods. J Virol Methods. 1997;67:161-166.
16. Chomczynski P, Mackey K, Draws R, Wilfinger W. DNAZol: a reagent for the rapid isolation of genomic DNA. BioTechniques 1997;22:550-553.
17. Pitcher DG, Saunders NA, Owen RJ. Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol. 1989;8:151-156.
18. de Groote MA, Visvesvara G, Wilson ML, et al. Polymerase chain reaction and culture confirmation of disseminated Encephalitozoon cuniculi in a patient with AIDS: successful therapy with albendazole. J Infect Dis. 1995;171:1375-1378.
19. Visvesvara GS, Leitch GJ, da Silva AJ, et al. Polyclonal and monoclonal antibody and PCR-amplified small subunit rRNA identification of a microsporidian, Encephalitozoon hellem, isolated from an AIDS patient with disseminated infection. J Clin Microbiol 1994;32:2760-2768.
20. Da Silva AJ, Slemenda SB, Visvesvara GS, et al. Detection of Septata intestinalis (microsporidia) Cali et al. 1993 using polymerase chain reaction primers targeting the small subunit ribosomal RNA coding region. Mol Diagn. 1997;2:47-52.