Localization of the transglutaminase cross-linking sites in the Bacillus subtilis spore coat protein GerQ

Journal of Bacteriology

Volumn 188, Issue 21, 2006, Pages 7609-7616

  Monroe, Alicia ^a   Setlow, Peter ^a  

^a UNIVERSITY OF CONNECTICUT HEALTH CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; GLUTAMINE; LYSINE; PEPTIDOGLYCAN; PROTEIN CWLJ; PROTEIN GERQ; PROTEIN GLUTAMINE GAMMA GLUTAMYLTRANSFERASE; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; BACILLUS SUBTILIS; BACTERIAL SPORE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN CROSS LINKING; PROTEIN LOCALIZATION; PROTEIN STRUCTURE; SPOROGENESIS; STRUCTURE ANALYSIS;

AMINO ACID SEQUENCE; BACILLUS SUBTILIS; BACTERIAL PROTEINS; BIOPOLYMERS; BLOTTING, WESTERN; GLUTAMINE; HYDROLASES; LYSINE; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; SEQUENCE ALIGNMENT; SPORES, BACTERIAL; TRANSGLUTAMINASES;

BACILLUS SUBTILIS;

EID: 33750463770     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.01116-06     Document Type: Article

References (26)

1. Anagnostopoulos, C., and J. Spizizen. 1961. Requirements for transformation in Bacillus subtilis. J. Bacteriol. 81:741-746.
2. Aronson, A. I., and P. Fitz-James. 1976. Structure and morphogenesis of the bacterial spore coat. Bacteriol. Rev. 40:360-402.
3. Carroll, T. M., and P. Setlow. 2005. Site-directed mutagenesis and structural studies suggest that the germination protease, GPR, in spores of Bacillus species is an atypical aspartic acid protease. J. Bacteriol. 187:7119-7125.
4. Cutting, S. M., and P. B. Vander Horn. 1990. Genetic analysis, p. 27-74. In C. R. Harwood and S. M. Cutting (ed.), Molecular biological methods for Bacillus. John Wiley & Sons Ltd., Chichester, England.
5. Driks, A. 1999. Bacillus subtilis spore coat. Microbiol. Mol. Biol. Rev. 63:1-20.
6. Driks, A. 2002. Maximum shields: the assembly and function of the bacterial spore coat. Trends Microbiol. 10:251-254.
7. Driks, A. 2002. Proteins of the spore core and coat, p. 527-535. In A. L. Sonenshein, J. A. Hoch, and R. Losick (ed.), Bacillus subtilis and its closest relatives: from genes to cells. ASM Press, Washington, D.C.
8. Henriques, A. O., B. W. Beall, and C. P. Moran, Jr. 1997. CotM of Bacillus subtilis, a member of the alpha-crystallin family of stress proteins, is induced during development and participates in spore outer coat formation. J. Bacteriol. 179:1887-1897.
9. Henriques, A. O., and C. P. Moran, Jr. 2000. Structure and assembly of the bacterial endospore coat. Methods 20:95-110.
10. Kobayashi, K., K. Hashiguchi, K. Yokozeki, and S. Yamanaka. 1998. Molecular cloning of the transglutaminase gene from Bacillus subtilis and its expression in Escherichia coli. Biosci. Biotechnol. Biochem. 62:1109-1114.
11. Kobayashi, K., Y. Kumazawa, K. Miwa, and S. Yamanaka. 1996. ε-(γ-Glutamyl)lysine cross-links of spore coat proteins and transglutaminase activity in Bacillus subtilis. FEMS Microbiol. Lett. 144:157-160.
12. Kobayashi, K., S. I. Suzuki, Y. Izawa, K. Miwa, and S. Yamanaka. 1998. Transglutaminase in sporulating cells of Bacillus subtilis. J. Gen. Appl. Microbiol. 44:85-91.
13. Kuwana, R., N. Okuda, H. Takamatsu, and K. Watabe. 2006. Modification of GerQ reveals a functional relationship between Tgl and YabG in the coat of Bacillus subtilis spores. J. Biochem. (Tokyo) 139:887-901.
14. Lorand, L., and R. M. Graham. 2003. Transglutaminases: crosslinking enzymes with pleiotropic functions. Nat. Rev. Mol. Cell Biol. 4:140-156.
15. Maniatis, T., E. F. Fritsch, and J. Sambrook. 1982. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
16. Nicholson, W. L., and P. Setlow. 1990. Sporulation, germination and outgrowth, p. 391-450. In C. R. Harwood and S. M. Cutting (ed.), Molecular biological methods for Bacillus. John Wiley & Sons Ltd., Chichester, England.
17. 2+-dipicolinate. J. Bacteriol. 183:4886-4893.
18. 2+-dipicolinate. J. Bacteriol. 185:2315-2329.
19. Ragkousi, K., and P. Setlow. 2004. Transglutaminase-mediated cross-linking of GerQ in the coats of Bacillus subtilis spores. J. Bacteriol. 186:5567-5575.
20. Setlow, P. 1994 Mechanisms which contribute to the long-term survival of spores of Bacillus species. Soc. Appl. Bacteriol. Symp. Ser. 23:49S-60S.
21. Setlow, P. 2003. Spore germination. Curr. Opin. Microbiol. 6:550-556.
22. Setlow, P. 2006. Spores of Bacillus subtilis: their resistance to and killing by radiation, heat and chemicals. J. Appl. Microbiol. 101:514-525.
23. Suzuki, S., Y. Izawa, K. Kobayashi, Y. Eto, S. Yamanaka, K. Kubota, and K. Yokozeki. 2000. Purification and characterization of novel transglutaminase from Bacillus subtilis spores. Biosci. Biotechnol. Biochem. 64:2344-2351.
24. Zhang, J., P. C. Fitz-James, and A. I. Aronson. 1993. Cloning and characterization of a cluster of genes encoding polypeptides present in the insoluble fraction of the spore coat of Bacillus subtilis. J. Bacteriol. 175:3757-3766.
25. Zilhão, R., R. Isticato, L. O. Martins, L. Steil, U. Völker, E. Ricca, C. P. Moran, Jr., and A. O. Henriques. 2005. Assembly and function of a spore coat-associated transglutaminase of Bacillus subtilis. J. Bacteriol. 187:7753-7764.
26. Zilhão, R., M. Serrano, R. Isticato, E. Ricca, C. P. Moran, Jr., and A. O. Henriques. 2004. Interactions among CotB, CotG, and CotH during assembly of the Bacillus subtilis spore coat. J. Bacteriol. 186:1110-1119.