Unique classes of mutations in the Saccharomyces cerevisiae G-protein translation elongation factor 1A suppress the requirement for guanine nucleotide exchange

Genetics

Volumn 174, Issue 2, 2006, Pages 651-663

  Ozturk, Sedide B ^a   Vishnu, Melanie R ^a   Olarewaju, Olubunmi ^a   Starita, Lea M ^a   Masison, Daniel C ^b   Kinzy, Terri Goss ^a  

^a RUTGERS ROBERT WOOD JOHNSON MEDICAL SCHOOL   (United States)

^b NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ELONGATION FACTOR 1ALPHA; GUANINE NUCLEOTIDE BINDING PROTEIN; GUANINE NUCLEOTIDE EXCHANGE FACTOR;

ARTICLE; CELL GROWTH; CONTROLLED STUDY; CRYSTAL STRUCTURE; FUNGAL STRAIN; GENE REPRESSION; MUTATIONAL ANALYSIS; NONHUMAN; NUCLEOTIDE BINDING SITE; PHENOTYPE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN STRUCTURE; SACCHAROMYCES CEREVISIAE; WESTERN BLOTTING;

BINDING SITES; GUANINE NUCLEOTIDE EXCHANGE FACTORS; MUTAGENESIS; PEPTIDE ELONGATION FACTOR 1; PROTEIN STRUCTURE, TERTIARY; SACCHAROMYCES CEREVISIAE PROTEINS;

EUKARYOTA; SACCHAROMYCES CEREVISIAE;

EID: 33750446298     PISSN: 00166731     EISSN: 00166731     Source Type: Journal    
DOI: 10.1534/genetics.106.059899     Document Type: Article

References (44)

1. ANAND, M., L. VALENTE, A. CARR-SCHMID, R. MUNSHI, O. OLAREWAJU et al., 2001 Functions of the translation elongation factor 1 in the yeast Saccharomyces cerevisiae. Symp. Quant. Biol. 66: 439-448.
2. ANDERSEN, G. R., L. PEDERSEN, L. VALENTE, I. CHATTERJEE, T. G. KINZY et al., 2000 Structural basis for nucleotide exchange and competition with tRNA in the yeast elongation factor complex eEF1A:eEF1Bα. Mol. Cell 6: 1261-1266.
3. ANDERSEN, G. R., L. VALENTE, L. PEDERSEN, T. G. KINZY and J. NYBORG, 2001 Crystal structures of nucleotide exchange intermediates in the eEF1A-eEF1Balpha complex. Nat. Struct. Biol. 8: 531-534.
4. BOEKE, J. D., J. TRUEHEART, G. NATSOULIS and G. R. FINK, 1987 5-Fluoroorotic acid as a selective agent in yeast molecular genetics. Methods Enzymol. 154: 164-175.
5. BOESEN, T., S. S. MOHAMMAD, G. D. PAVITT and G. R. ANDERSEN, 2004 Structure of the catalytic fragment of translation initiation factor 2B and identification of a critically important catalytic residue. J. Biol. Chem. 279: 10584-10592.
6. BOURNE, H. R., D. A. SANDERS and F. MCCORMICK, 1991 The GTPase superfamily: conserved structure and molecular mechanism. Nature 349: 117-127.
7. CARR-SCHMID, A., N. DURKO, J. CAVALLIUS, W. C. MERRICK and T. G. KINZY, 1999a Mutations in a GTP-binding motif of eEF1A reduce both translational fidelity and the requirement for nucleotide exchange. J. Biol. Chem. 274: 30297-30302.
8. CARR-SCHMID, A., L. VALENTE, V. I. LOIK, T. WILLIAMS, L. M. STARITA et al., 1999b Mutations in elongation factor 1b, a guanine nucleotide exchange factor, enhance translational fidelity. Mol. Cell. Biol. 19: 5257-5266.
9. CHANG, Y. W., and J. A. TRAUGH, 1997 Phosphorylation of elongation factor 1 and ribosomal protein S6 by multipotential S6 kinase and insulin stimulation of translational elongation. J. Biol. Chem. 272: 28252-28257.
10. CHANG, Y. W., and J. A. TRAUGH, 1998 Insulin stimulation of phosphorylation of elongation factor 1 (eEF-1) enhances elongation activity. Eur. J. Biochem. 251: 201-207.
11. CHEN, J. J., 2000 Heme-regualted eIF2α kinase, pp. 529-546 in Translational Control of Gene Expression, edited by N. SONENBERG, J. W. B. HERSHEY and M. B. MATHEWS. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
12. COPELAND, P. R., 2003 Regulation of gene expression by stop codon recoding: selenocysteine. Gene 312: 17-25.
13. DE NADAL, E., R. P. FADDEN, A. RUIZ, T. HAYSTEAD and J. ARINO, 2001 A role for the ppz ser/thr protein phosphatases in the regulation of translation elongation factor 1balpha. J. Biol. Chem. 276: 14829-14834.
14. DINMAN, J. D., and T. G. KINZY, 1997 Translational misreading: mutations in translation elongation factor 1a differentially affect programmed ribosomal frameshifting and drug sensitivity. RNA 3: 870-881.
15. GOMEZ, E., S. S. MOHAMMAD and G. D. PAVITT, 2002 Characterization of the minimal catalytic domain within eIF2B: the guanine-nucleotide exchange factor for translation initiation. EMBO J. 21: 5292-5301.
16. GROMADSKI, K., H. WIEDEN and M. RODNINA, 2002 Kinetic mechanism of elongation factor Ts-catalyzed nucleotide exchange in elongation factor Tu. Biochemistry 41: 162-169.
17. HARGER, J. W., and J. D. DINMAN, 2003 An in vivo dual-luciferase assay system for studying translational recoding in the yeast Saccharomyces cerevisiae. RNA 9: 1019-1024.
18. HERSHEY, J. W. B., and W. C. MERRICK, 2000 The pathway and mechanism of initiation of protein synthesis, pp. 33-88 in Translational Control of Gene Expression, edited by N. Sonenberg, J. W. B. Hershey and M. B. Mathews. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
19. HERSKOWITZ, I., and R. E. JENSEN, 1991 Putting the HO gene to work: practical uses for mating-type switching. Methods Enzymol. 194: 132-146.
20. HINNEBUSCH, A. G., 2000 Mechanism and regulation of initiator methionyl-tRNA binding to ribosomes, pp. 185-244 in Translational Control of Gene Expression, edited by N. Sonenberg, J. W. B. HERSHEY and M. B. MATHEWS. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
21. HIRAGA, K., K. SUZUKI, E. TSUCHIYA and T. MIYAKAWA, 1993 Cloning and characterization of the elongation factor EF-1β homologue of Saccharomyces cerevisiae. EF-1β is essential for growth. FEBS Lett. 316: 165-169.
22. ITO, H., Y. FUKUDA, K. MURATA and A. KIMURA, 1983 Transformation of intact yeast cells treated with alkali cations. J. Bacteriol. 153: 163-168.
23. JANSSEN, G. M. C., G. D. F. MAESSEN, R. AMONS and W. MOLLER, 1988 Phosphorylation of elongation factor 1b by an exogenous kinase affects its catalytic nucleotide exchange activity. J. Biol. Chem. 263: 11063-11066.
24. KAUFMAN, R. J., 2000 Double-stranded RNA-activated protein kinase PKR, pp. 503-528 in Translational Control of Gene Expression, edited by N. SONENBERG, J. W. B. HERSHEY and M. B. MATHEWS. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
25. KAWASHIMA, T., C. BERTHET-COLOMINAS, M. WULFF, S. CUSACK and R. LEBERMAN, 1996 The structure of the Escherichia coli EF-Tu·EF-Ts complex at 2.5Å resolution. Nature 379: 511-518.
26. KINZY, T. G., and J. L. WOOLFORD, JR., 1995 Increased expression of Saccharomyces cerevisiae translation elongation factor EF-1α bypasses the lethality of a TEF5 null allele encoding EF-1β. Genetics 141: 481-489.
27. LAWRENCE, C. W., 1991 Classical mutagenesis techniques. Methods Enzymol. 194: 273-281.
28. MORTIMER, R. K., and D. C. HAWTHORNE, 1966 Genetic mapping in Saccharomyces. Genetics 53: 165-173.
29. MUNSHI, R., K. A. KANDL, A. CARR-SCHMID, J. L. WHITACRE, A. E. ADAMS et al., 2001 Overexpression of translation elongation factor 1a affects the organization and function of the actin cytoskeleton in yeast. Genetics 157: 1425-1436.
30. OLAREWAJU, O., P. A. ORTIZ, W. CHOWDHURY, I. CHATTERJEE and T. G. KINZY, 2004 The translation elongation factor, eEF1B, plays a role in the oxidative stress response pathway. RNA Biol. 1: 12-17.
31. PETERS, H. I., Y.-W. E. CHANG and J. A. TRAUGH, 1995 Phosphorylation of elongation factor 1 (EF-1) by protein kinase C stimulates GDP/GTP-exchange activity. Eur. J. Biochem. 234: 550-556.
32. +2 and key lysine modulate exchange activity of eukaryotic translation elongation factor 1Bα. J. Biol. Chem. 281: 19457-19468.
33. RABINOW, L., S. L. CHIANG and J. A. BIRCHLER, 1993 Mutations at the Darkener of apricot locus modulate transcript levels of copia and copia-induced mutations in Drosophila melanogaster. Genetics 134: 1175-1185.
34. RODNINA, M. V., R. FRICKE, L. KUHN and W. WINTERMEYER, 1995 Codon-dependent conformational change of elongation factor Tu preceding GTP hydrolysis on the ribosome. EMBO J. 14: 2613-2619.
35. RON, D., and H. P. HARDING, 2000 PERK and translational control by stress in the endoplasmic reticulum, pp. 547-560 in Translational Control of Gene Expression, edited by N. Sonenberg, J. W. B. HERSHEY and M. B. MATHEWS. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
36. SALAS-MARCO, J., and D. M. BEDWELL, 2005 Discrimination between defects in elongation fidelity and termination efficiency provides mechanistic insights into translational readthrough. J. Mol. Biol. 348: 801-815.
37. SAMBROOK, J., E. F. FRITSCH and T. MANIATIS, 1989 Molecular Cloning. A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
38. SANDBAKEN, M. G., and M. R. CULBERTSON, 1988 Mutations in elongation factor EF-1α affect the frequency of frameshifting and amino acid misincorporation in Saccharomyces cerevisiae. Genetics 120: 923-934.
39. SHERMAN, F., G. R. FINK and J. B. HICKS, 1986 Methods in Yeast Genetics: A Laboratory Course Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
40. 2p: implications for the interaction with effector proteins. Biochemistry 44: 2225-2236.
41. TOYN, J. H., P. L. GUNYUZLU, W. H. WHITE, L. A. THOMPSON and G. F. HOLLIS, 2000 A counterselection for the tryptophan pathway in yeast: 5-fluoroanthranilic acid resistance. Yeast 16: 553-560.
42. VAN DAMME, H. T. F., R. KARSSIES, C. J. TIMMERS, G. M. C. JANSSEN and W. MOLLER, 1990 Elongation factor 1β of artemia: localization of functional sites and homology to elongation factor 1δ. Biochim. Biophys. Acta 1050: 241-247.
43. VETTER, I. R., and A. WITTENGHOFER, 2001 The guanine nucleotide-binding switch in three dimensions. Science 294: 1299-1304.
44. ZAVIALOV, A. V., V. V. HAURYLIUK and M. EHRENBERG, 2005 Guanine-nucleotide exchange on ribosome-bound elongation factor G initiates the translocation of tRNAs. J. Biol. 4: 9.