Possible errors in the interpretation of ethidium bromide and picogreen DNA staining results from ethidium monoazide-treated DNA [2]

Applied and Environmental Microbiology

Volumn 72, Issue 10, 2006, Pages 6860-6861

  Hein, Ingeborg ^a   Flekna, Gabriele ^a   Wagner, Martin ^a  

^a UNIVERSITY OF VETERINARY MEDICINE   (Austria)

Author keywords

[No Author keywords available]

Indexed keywords

AGAROSE; AZIDE; BACTERIAL DNA; CHEMICAL AGENT; ETHIDIUM; ETHIDIUM AZIDE; ETHIDIUM BROMIDE; INTERCALATING AGENT; UNCLASSIFIED DRUG;

AGAR GEL ELECTROPHORESIS; ARTIFACT; BACTERIAL CELL; CELL VIABILITY; COMPARATIVE STUDY; DNA BINDING; DNA DETERMINATION; DNA EXTRACTION; DNA HELIX; DNA ISOLATION; ESCHERICHIA COLI; FLUORESCENCE; LETTER; LIGHT EXPOSURE; NONHUMAN; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; SALMONELLA ENTERICA; SPECTROPHOTOMETRY; STAINING; ULTRAVIOLET RADIATION;

AZIDES; BIAS (EPIDEMIOLOGY); DNA; ETHIDIUM; ORGANIC CHEMICALS; STAINING AND LABELING;

EID: 33750061026     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.01243-06     Document Type: Letter

References (6)

1. Bolton, P. H., and D. R. Kearns. 1978. Spectroscopic properties of ethidium monoazide: a fluorescent photoaffinity label for nucleic acids. Nucleic Acids Res. 5:4891-4903.
2. Nocker, A., and A. K. Camper. 2006. Selective removal of DNA from dead cells of mixed bacterial communities by use of ethidium monoazide. Appl. Environ. Microbiol. 72:1997-2004.
3. Nogva, H. K., S. M. Drømtørp, H. Nissen, and K. Rudi. 2003. Ethidium monoazide for DNA-based differentiation of viable and dead bacteria by 5′-nuclease PCR. BioTechniques 34:804-813.
4. Rudl, K., H. K. Nogva, B. Moen, H. Nissen, S. Bredholt, T. Møretrø, K. Naterstad, and A. Holck. 2002. Development and application of new nucleic acid-based technologies for microbial community analyses in foods. Int. J. Food Microbiol. 78:171-180.
5. Yielding, L. W., and W. J. Firth III. 1980. Structure-function characterization for ethidium photoaffinity labels as mutagens in Salmonella. Mutat. Res. 71:161-168.
6. Zipper, H., H. Brunner, J. Bernhagen, and F. Vitzthum. 2004. Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications. Nucleic Acids Res. 32:e103.