Generation of useful insertionally blocked sterol degradation pathway mutants of fast-growing mycobacteria and cloning, characterization, and expression of the terminal oxygenase of the 3-ketosteroid 9α-hydroxylase in Mycobacterium smegmatis mc2155

Applied and Environmental Microbiology

Volumn 72, Issue 10, 2006, Pages 6554-6559

  Andor, Attila ^a   Jekkel, Antónia ^a   Hopwood, David A ^b   Jeanplong, Ferenc ^a   Ilkoy, Éva ^a   Kónya, Attila ^a   Kurucz, István ^a   Ambrus, Gábor ^a  

^a Institute for Drug Research Budapest   (Hungary)

^b JOHN INNES CENTRE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CHROMOSOMES; DNA; ENZYME KINETICS; ESCHERICHIA COLI; GENES; HYDROXYLATION; MUTAGENS;

MYCOBACTERIUM SMEGMATIS; STEROID RING; STEROL DEGRADATION;

MICROBIOLOGY;

23,24 DINORCHOLANE; 3 KETOSTEROID 9ALPHA HYDROXYLASE; ANDROSTENEDIONE; CHOLANE DERIVATIVE; GENOMIC DNA; OXYGENASE; SITOSTEROL; STEROL; UNCLASSIFIED DRUG;

BACTERIUM; DEGRADATION; MUTATION; STEROL;

ARTICLE; BACTERIAL GROWTH; BACTERIAL STRAIN; BACTERIUM IDENTIFICATION; DEGRADATION; DNA FLANKING REGION; ESCHERICHIA COLI; GENE CONSTRUCT; GENE INSERTION; GENE LIBRARY; HYDROXYLATION; MOLECULAR CLONING; MUTANT; MYCOBACTERIUM; MYCOBACTERIUM PHLEI; MYCOBACTERIUM SMEGMATIS; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PLASMID; PROTEIN DOMAIN; PROTEIN EXPRESSION; RECOMBINANT PLASMID; STEROIDOGENESIS; TEMPERATURE SENSITIVITY; TRANSPOSON;

CLONING, MOLECULAR; CLONING, ORGANISM; GENE EXPRESSION; MOLECULAR SEQUENCE DATA; MYCOBACTERIUM SMEGMATIS; OXYGENASES; STEROLS;

CORYNEBACTERINEAE; ESCHERICHIA COLI; MYCOBACTERIUM PHLEI; MYCOBACTERIUM SMEGMATIS;

EID: 33750046643     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.00941-06     Document Type: Article

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