Evaluation and applicability of a purification method coupled with nested PCR for the detection of Toxoplasma oocysts in water

Letters in Applied Microbiology

Volumn 43, Issue 5, 2006, Pages 475-481

  Kourenti, C ^a   Karanis, P ^a,b  

^a UNIVERSITY OF COLOGNE   (Germany)

^b OBIHIRO UNIVERSITY OF AGRICULTURE AND VETERINARY MEDICINE   (Japan)

Author keywords

Detection; Flocculation; Oocysts; Polymerase chain reaction; Purification; Toxoplasma gondii; Water

Indexed keywords

PURIFICATION; RNA; SEAWATER; WATER POLLUTION; WATER RESOURCES;

'CURRENT; 18S RRNA; DETECTION; NESTED PCR; OOCYSTS; PURIFICATION METHOD; SUCROSE GRADIENTS; TAP WATER; TOXOPLASMA GONDII; WATER SAMPLES;

POLYMERASE CHAIN REACTION;

ALUMINUM SULFATE; PROTOZOAL DNA; RIVER WATER; RNA 18S; SEA WATER; TAP WATER;

CONTAMINATION; DNA; FLOCCULATION; SEPARATION; WATER ANALYSIS; WATER TREATMENT;

CYST; DETECTION METHOD; DNA; FLOCCULATION; POLYMERASE CHAIN REACTION; PROTOZOAN; RIVER WATER; SUCROSE;

ARTICLE; CONTROLLED STUDY; DNA DETERMINATION; EPIDEMIC; EVALUATION; FLOCCULATION; MICROORGANISM DETECTION; NONHUMAN; OOCYST; POLYMERASE CHAIN REACTION; PURIFICATION; SENSITIVITY AND SPECIFICITY; SUCROSE GRADIENT; TECHNIQUE; TOXOPLASMA GONDII; WATER CONTAMINATION; WATER QUALITY; WATER SAMPLING;

TOXOPLASMA; TOXOPLASMA GONDII;

EID: 33750008080     PISSN: 02668254     EISSN: 1472765X     Source Type: Journal    
DOI: 10.1111/j.1472-765X.2006.02008.x     Document Type: Article

References (16)

1. Arrowood, M.J. and Sterling, C.R. (1987) Isolation of Cryptosporidium oocysts and sporozoites using discontinuous sucrose and isopycnic Percoll gradients. J Parasitol 73, 314-319.
2. Bifulco, J.M. and Schaefer, F.W. Jr (1993) Antibody-magnetite method for selective concentration of Giardia lamblia cysts from water samples. Appl Environ Microbiol 59, 772-776.
3. Braid, M.D., Daniels, L.M. and Kitts, C.L. (2003) Removal of PCR inhibitors from soil DNA by chemical flocculation. J Microbiol Methods 52, 389-393.
4. Dubey, J.P. (2004) Toxoplasmosis - a waterborne zoonosis. Vet Parasitol 126, 57-72.
5. Dubey, J.P., Miller, N.L. and Frenkel, J.K. (1970) The Toxoplasma gondii oocyst from cat feces. J Exp Med 132, 636-662.
6. Dumetre, A. and Darde, M.L. (2003) How to detect Toxoplasma gondii oocysts in environmental samples? FEMS Microbiol Rev 27, 651-661.
7. Dumetre, A. and Darde, M.L. (2004) Purification of Toxoplasma gondii oocysts by cesium chloride gradient. J Microbiol Methods 56, 427-430.
8. Dumetre, A. and Darde, M.L. (2005) Immunomagnetic separation of Toxoplasma gondii oocysts using a monoclonal antibody directed against the oocyst wall. J Microbiol Methods 61, 209-217.
9. Johnson, D.W., Pieniazek, N.J., Griffin, D.W., Misener, L. and Rose, J.B. (1995) Development of a PCR protocol for sensitive detection of Cryptosporidium oocysts in water samples. Appl Environ Microbiol 61, 3849-3855.
10. Kourenti, C. and Karanis, P. (2004) Development of a sensitive polymerase chain reaction method for the detection of Toxoplasma gondii in water. Water Sci Technol 50, 287-291.
11. Kourenti, C., Heckeroth, A., Tenter, A. and Karanis, P. (2003) Development and application of different methods for the detection of Toxoplasma gondii in water. Appl Environ Microbiol 69, 102-106.
12. MacPherson, J.M. and Gajadhar, A.A. (1993) Sensitive and specific polymerase chain reaction detection of Toxoplasma gondii for veterinary and medical diagnosis. Can J Vet Res 57, 45-48.
13. Schwab, K.J. and McDevitt, J.J. (2003) Development of a PCR-enzyme immunoassay oligoprobe detection method for Toxoplasma gondii oocysts, incorporating PCR controls. Appl Environ Microbiol 69, 5819-5825.
14. Shepherd, K.M. and Wyn-Jones, A.P. (1996) An evaluation of methods for the simultaneous detection of Cryptosporidium oocysts and Giardia cysts from water. Appl Environ Microbiol 62, 1317-1322.
15. Sluter, S.D., Tzipori, S. and Widmer, G. (1997) Parameters affecting polymerase chain reaction detection of water-borne Cryptosporidium parvum oocysts. Appl Microbiol Biotechnol 48, 325-330.
16. Villena, I., Aubert, D., Gomis, P., Ferte, H., Inglard, J.C., Denis-Bisiaux, H., Dondon, J.M., Pisano, E. et al. (2004) Evaluation of a strategy for Toxoplasma gondii oocyst detection in water. Appl Environ Microbiol 70, 4035-4039.