Ionizing groups at the active site of the alkaline phosphatase from ostrea cucullate

Tsinghua Science and Technology

Volumn 11, Issue 4, 2006, Pages 470-474

  Chen, Qiao ^a   Wang, Qin ^a   Liao, Jinhua ^a   Chen, Qingxi ^a  

^a XIAMEN UNIVERSITY   (China)

Author keywords

Alkaline phosphatase; Chemical modification; Ionizing groups; Lysine residue; Ostrea cucullate

Indexed keywords

CHEMICAL MODIFICATION; ENTHALPY; IONIZATION; ORGANIC SOLVENTS;

ALKALINE PHOSPHATASE; IONIZING GROUPS; LYSINE RESIDUE; OSTREA CUCULLATE;

ENZYMES;

EID: 33749819221     PISSN: 10070214     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1007-0214(06)70219-X     Document Type: Article

References (15)

1. Cao Zhifang, Xu Zhen, Park Yongdoo, Zhou Haimeng. Activation of calf intestinal alkaline phosphatase by trifluoroethanol. Tsinghua Science and Technology, 2001, 6(5): 426-431.
2. Zhang Yingxia, Yan Hulian, Liu Yongli, Xi Hongwei, Zhou Haimeng. Comparison of inactivation and unfolding of calf intestinal alkaline phosphatase in guanidinium chloride solution. Tsinghua Science and Technology, 2002, 7(4): 425-429.
3. Hamilton T A, Gornicki S Z, Sussman H H. Alkaline phosphates from human milk. Comparison with isoenzymes from placenta and liver. Biochem. J., 1979, 177: 197-201.
4. Zhavoronkova M, Muginova S V, Veselova I A, Shekhovtsova T N. Determination of zinc (II) using native and immobilized alkaline phosphatases of different origin. J. Analytical Chem., 2003, 58: 77-84.
5. Kim E E, Wyckoff H W. Reaction mechanism of alkaline phosphatase based on crystal structures. Two-metal ion catalysis. J. Mol. Biol., 1991, 218: 449-464.
6. Xiao R, Xie L P, Lin J Y, Li C H, Chen Q X, Zhou H M, Zhang R Q. Purification and enzymatic characterization of alkaline phosphatase from Pinctada fucata. J. Mol. Catalysis B-Enzymatic, 2002, 17(2): 65-74.
7. Rein T W, Wilson I B. In: Boyer P D, ed. The Enzymes, 3rd Ed. New York: Academic Press, 1971: 373-416.
8. Chappelet T D. Intestinal alkaline phosphatase catalytic properties and half of the sites reactivity. Biochem., 1974, 13: 1789-1795.
9. Chen Qingxi, Yan Sixu. The essential groups of the amphioxus alkaline phosphatase. J. Xiamen Univ. (Natural Sci.), 1986, 25(5): 568-573.
10. Chen Qingxi, Zhao Hong, Zhang Wei, Yang Huanghao, Zhuang Zonglai, Zhang Tong, Zhou Haimeng. Unfolding and inactivation of green crab (Scylla serrata) alkaline phosphatase during thermal denaturation. Tsinghua Science and Technology, 1998, 3(3): 1059-1062.
11. Chen Qiao, Chen Qingxi, Lin Jiancheng, Liao Jinhua. Studies on properties of alkaline phosphatase from Ostrea cucullate. J. Oceanography in Taiwan Strait, 2003, 22(4): 475-479.
12. Chen Q X, Zhang W, Zheng W Z, Zhao H, Yan S H, Wang H R, Zhou H M. Kinetic of inhibition of alkaline phosphatase from green crab (Scylla serrata) by Nbromosuccinimide. J. Protein Chem., 1996, 15: 345-350.
13. Chen Q X, Zhou H M. An essential lysine residue of green crab (Scylla serrata) alkaline phosphatase. Biochem. Mol. Biol. Int., 1998, 46: 225-231.
14. Rapala-Kozik M, Kozik A. Mechanism of ligand-protein interaction in plant seed thiamine-binding proteins. Preliminary chemical identification of amino acid residues essential for thiamine binding to the buckwheat-seed protein. Biochimie, 1996, 78: 77-84.
15. Fishman W H, Ghosh N K. Influence of reagents reacting with metal, thiol amino sites on catalytic activity and Lphenylalanine of rat intestinal alkaline phosphatase. Biochem J., 1967, 105: 1163-1170.