Chemical reaction at specific sites and reaction-induced self-assembly as observed by in situ and real time SANS: Enzymatic polymerization to synthetic cellulose

Biomacromolecules

Volumn 7, Issue 9, 2006, Pages 2479-2482

  Hashimoto, Takeji ^a,b   Tanaka, Hirokazu ^a,b   Koizumi, Satoshi ^a   Kurosaki, Kazuhiro ^b   Ohmae, Masashi ^b   Kobayashi, Shiro ^b,c  

^a JAPAN ATOMIC ENERGY AGENCY   (Japan)

^b KYOTO UNIVERSITY   (Japan)

^c KYOTO INSTITUTE OF TECHNOLOGY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

DIFFUSION-LIMITED ASSOCIATION; ENZYMATIC POLYMERIZATION; SMALL-ANGLE NEUTRON SCATTERING (SANS); SURFACE FRACTAL DIMENSION; SYNTHESIZED MOLECULES;

ASSOCIATION REACTIONS; BIOSYNTHESIS; CELLULOSE; ENZYME KINETICS; NEUTRON SCATTERING; POLYMERIZATION; SELF ASSEMBLY;

MACROMOLECULES;

CELLULASE; CELLULOSE;

ARTICLE; CHEMICAL REACTION KINETICS; CONTROLLED STUDY; CRYSTALLIZATION; DIFFUSION; FRACTAL ANALYSIS; NEUTRON SCATTERING; POLYMERIZATION; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN ASSEMBLY; REACTION TIME;

BIOCOMPATIBLE MATERIALS; BIOTECHNOLOGY; CELLULASE; CELLULOSE; CHEMISTRY, ORGANIC; CHEMISTRY, PHYSICAL; DIFFUSION; FRACTALS; MACROMOLECULAR SUBSTANCES; NEUTRONS; PHYSICOCHEMICAL PHENOMENA; POLYMERS; SCATTERING, RADIATION; SOLVENTS; SURFACE PROPERTIES;

EID: 33749625286     PISSN: 15257797     EISSN: None     Source Type: Journal    
DOI: 10.1021/bm0605255     Document Type: Article

References (19)

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16. During the purification procedures of the crude enzyme to obtain the active enzyme with assaying the polymerization process, the total weight of the starting enzyme was much reduced to less than 1 wt % (approximately 0.1 wt %) after isolation of the purified active enzyme fraction.
17. w are estimated to be quite large!
18. n, of the reaction product was determined to be 1840 by size exclusion chromatography, corresponding to DP = 11 in the glucose unit, as will be detailed elsewhere. Generally in carbohydrate chemistry, a "polysaccharide" refers to a substance having more than 10 monosaccharide units [Kennedy, J. F.; White, C. A. Polysaccharides. In Comprehensive Organic Chemistry; Barton, D., Ollis, W. D., Haslam, E., Eds.; Pergamon Press: Oxford, 1979; Vol. 5, pp 755-830]. According to this terminology, the present product belongs to the cellulose, a typical polysaccharide, family.
19. -3. Nevertheless, we anticipate there is enough free space in the assembly for diffusion of monomers and reaction-terminated polymers.