Improvement of methods for the isolation of cell-free fetal DNA from maternal plasma: Comparison of a manual and an automated method

Annals of the New York Academy of Sciences

Volumn 1075, Issue , 2006, Pages 308-312

  Huang, Dorothy J ^a   Zimmermann, Bernhard G ^a,b   Holzgreve, Wolfgang ^a   Hahn, Sinuhe ^a  

^a UNIVERSITY WOMEN S HOSPITAL   (Switzerland)

^b UNIVERSITY OF THE WEST OF ENGLAND   (United Kingdom)

Author keywords

Cell free fetal DNA; DNA extraction; Maternal plasma

Indexed keywords

BIOLOGICAL MARKER; DNA; FETAL DEOXYRIBONUCLEIC ACID; UNCLASSIFIED DRUG;

AUTOMATION; CELL FREE SYSTEM; CONFERENCE PAPER; DIAGNOSTIC VALUE; DNA EXTRACTION; DNA ISOLATION; FEMALE; FETOPLACENTAL UNIT; HUMAN; HUMAN EXPERIMENT; INTERMETHOD COMPARISON; MATERNAL PLASMA; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS; TECHNIQUE;

EID: 33749540878     PISSN: 00778923     EISSN: 17496632     Source Type: Book Series    
DOI: 10.1196/annals.1368.041     Document Type: Conference Paper

References (8)

1. HAHN, S. & W. HOLZGREVE. 2002. Prenatal diagnosis using fetal cells and cell-free fetal DNA in maternal blood: what is currently feasible? Clin. Obstet. Gynecol. 45: 649-656.
2. CHIU, R. W. & Y.M. LO. 2004. The biology and diagnostic applications of fetal DNA and RNA in maternal plasma. Curr. Top. Dev. Biol. 61: 81-111.
3. WATAGANARA, T. & D.W. BIANCHI. 2002. Fetal cell-free nucleic acids in the maternal circulation: new clinical applications. Ann. N.Y. Acad. Sci. 1022: 90-99.
4. LO, Y.M. et al. 1998. Quantitative analysis of fetal DNA in maternal plasma and serum: implications for noninvasive prenatal diagnosis. Am. J. Hum. Genet. 62: 768-775.
5. Li, Y. et al. 2004. Size separation of circulatory DNA in maternal plasma permits ready detection of fetal DNA polymorphisms. Clin. Chem. 50: 1002-1011.
6. ZHONG, X.Y. et al. 2000. Detection of fetal Rhesus D and sex using fetal DNA from maternal plasma by multiplex polymerase chain reaction. Br. J. Obstet. Gynaecol. 107: 766-769.
7. ZIMMERMANN, B. et al. 2005. Optimized real-time quantitative PCR measurement of male fetal DNA in maternal plasma. Clin. Chem. 51: 1598-1604.
8. CHAN, K.C. et al. 2004. Size distributions of maternal and fetal DNA in maternal plasma. Clin. Chem. 50: 88-92.