An Integrated High-Throughput System for mRNA Purification and Quantitation for Use in Identifying Gene Knockdown by RNA Interference

Journal of Laboratory Automation

Volumn 11, Issue 5, 2006, Pages 314-318

  Wang, Danhui ^a   Douglas, Derek ^a   Kreader, Carol ^a   Van Dinther, Jennifer ^a   Valdes Camin, Rafael ^a  

^a Sigma Aldrich Co   (United States)

Author keywords

expression; high throughput; knockdown of gene; mRNA isolation; quantitative; RNA interference; RT PCR

Indexed keywords

GENE KNOCKDOWN; INTEGRATED HIGH-THROUGHPUT SYSTEM; QUANTITATIVE REVERSE TRANSCRIPTIONAL PCR; RNA INTERFERENCE;

AUTOMATION; CELLS; CHEMICAL ANALYSIS; CLINICAL LABORATORIES; GENETIC ENGINEERING; RNA;

BIOMEDICAL ENGINEERING;

I KAPPA B KINASE; INTERLEUKIN 1 RECEPTOR ASSOCIATED KINASE 1; INTERLEUKIN 1BETA; LAMIN A; LAMIN C; MESSENGER RNA; MITOGEN ACTIVATED PROTEIN KINASE 3; POLYADENYLATED RNA; SMALL INTERFERING RNA; STRESS ACTIVATED PROTEIN KINASE 1;

ACCURACY; ARTICLE; CELL CULTURE; COMPUTER PROGRAM; COMPUTER SYSTEM; CONTROLLED STUDY; GENE EXPRESSION; GENE EXPRESSION PROFILING; GENE IDENTIFICATION; GENETIC TRANSCRIPTION; GENETIC TRANSFECTION; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; LABORATORY AUTOMATION; MAMMAL CELL; METHODOLOGY; QUANTITATIVE ANALYSIS; REPRODUCIBILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; RNA INTERFERENCE; RNA ISOLATION; RNA PURIFICATION; SENSITIVITY ANALYSIS; VALIDATION STUDY;

EID: 33749512757     PISSN: 22110682     EISSN: 15402452     Source Type: Journal    
DOI: 10.1016/j.jala.2006.07.003     Document Type: Article

References (2)

1. Chatterjee-Kishore M. From genome to phenome—RNAi library screening and hit characterization using signaling pathway analysis. Curr. Opin. Drug Discov. Dev. 2006, 9(2), 231–239.
2. Short Protocols in Molecular Biology; Ausubel F. Brent R. Kingston R. E. Moore D. D. Seidman J. G. Smith J. A. Struhl K., Eds.; 3rd ed. John Wiley & Sons, Inc.: New York, NY; 1997; pp 1–12 Chapter 4.